Experiment: MRS + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16140 and BBR_RS16145 are separated by 75 nucleotides BBR_RS16145 and BBR_RS16150 are separated by 145 nucleotides
BBR_RS16140: BBR_RS16140 - long-chain fatty acid--CoA ligase, at 1,368,687 to 1,370,747
_RS16140
BBR_RS16145: BBR_RS16145 - hypothetical protein, at 1,370,823 to 1,371,311
_RS16145
BBR_RS16150: BBR_RS16150 - GuaB3 family IMP dehydrogenase-related protein, at 1,371,457 to 1,372,581
_RS16150
Position (kb)
1370
1371
1372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1369.896 kb on + strand, within BBR_RS16140 at 1369.896 kb on + strand, within BBR_RS16140 at 1369.897 kb on + strand, within BBR_RS16140 at 1369.898 kb on + strand, within BBR_RS16140 at 1370.029 kb on - strand, within BBR_RS16140 at 1370.029 kb on - strand, within BBR_RS16140 at 1370.088 kb on + strand, within BBR_RS16140 at 1370.158 kb on + strand, within BBR_RS16140 at 1370.177 kb on + strand, within BBR_RS16140 at 1370.177 kb on + strand, within BBR_RS16140 at 1370.185 kb on + strand, within BBR_RS16140 at 1370.193 kb on - strand, within BBR_RS16140 at 1370.233 kb on - strand, within BBR_RS16140 at 1370.246 kb on + strand, within BBR_RS16140 at 1370.286 kb on + strand, within BBR_RS16140 at 1370.345 kb on - strand, within BBR_RS16140 at 1370.350 kb on - strand, within BBR_RS16140 at 1370.387 kb on - strand, within BBR_RS16140 at 1370.390 kb on + strand, within BBR_RS16140 at 1370.426 kb on - strand, within BBR_RS16140 at 1370.426 kb on - strand, within BBR_RS16140 at 1370.436 kb on + strand, within BBR_RS16140 at 1370.526 kb on - strand, within BBR_RS16140 at 1370.538 kb on - strand, within BBR_RS16140 at 1370.538 kb on - strand, within BBR_RS16140 at 1370.553 kb on + strand at 1370.558 kb on + strand at 1370.560 kb on + strand at 1370.560 kb on + strand at 1370.568 kb on - strand at 1370.581 kb on + strand at 1370.613 kb on + strand at 1370.666 kb on + strand at 1370.738 kb on + strand at 1370.771 kb on + strand at 1370.771 kb on + strand at 1370.780 kb on - strand at 1370.805 kb on - strand at 1370.821 kb on + strand at 1370.842 kb on + strand at 1370.842 kb on + strand at 1370.850 kb on - strand at 1370.850 kb on - strand at 1370.908 kb on - strand, within BBR_RS16145 at 1370.916 kb on - strand, within BBR_RS16145 at 1370.930 kb on - strand, within BBR_RS16145 at 1370.988 kb on + strand, within BBR_RS16145 at 1371.017 kb on + strand, within BBR_RS16145 at 1371.028 kb on - strand, within BBR_RS16145 at 1371.036 kb on + strand, within BBR_RS16145 at 1371.044 kb on - strand, within BBR_RS16145 at 1371.046 kb on + strand, within BBR_RS16145 at 1371.054 kb on - strand, within BBR_RS16145 at 1371.064 kb on - strand, within BBR_RS16145 at 1371.067 kb on + strand, within BBR_RS16145 at 1371.237 kb on + strand, within BBR_RS16145 at 1371.263 kb on - strand at 1371.263 kb on - strand at 1371.279 kb on - strand at 1371.360 kb on + strand at 1371.368 kb on - strand at 1371.409 kb on + strand at 1371.458 kb on - strand at 1371.609 kb on - strand, within BBR_RS16150 at 1371.655 kb on - strand, within BBR_RS16150 at 1371.660 kb on - strand, within BBR_RS16150 at 1371.684 kb on - strand, within BBR_RS16150 at 1371.700 kb on - strand, within BBR_RS16150 at 1371.700 kb on - strand, within BBR_RS16150 at 1371.804 kb on + strand, within BBR_RS16150 at 1371.805 kb on - strand, within BBR_RS16150 at 1371.812 kb on - strand, within BBR_RS16150 at 1371.815 kb on - strand, within BBR_RS16150 at 1371.866 kb on - strand, within BBR_RS16150 at 1371.882 kb on - strand, within BBR_RS16150 at 1371.893 kb on - strand, within BBR_RS16150 at 1371.970 kb on + strand, within BBR_RS16150 at 1371.983 kb on + strand, within BBR_RS16150 at 1371.993 kb on - strand, within BBR_RS16150 at 1371.993 kb on - strand, within BBR_RS16150 at 1372.025 kb on - strand, within BBR_RS16150 at 1372.064 kb on + strand, within BBR_RS16150 at 1372.092 kb on - strand, within BBR_RS16150 at 1372.094 kb on - strand, within BBR_RS16150 at 1372.144 kb on - strand, within BBR_RS16150 at 1372.144 kb on - strand, within BBR_RS16150 at 1372.179 kb on + strand, within BBR_RS16150 at 1372.224 kb on + strand, within BBR_RS16150 at 1372.224 kb on + strand, within BBR_RS16150 at 1372.257 kb on + strand, within BBR_RS16150
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time48hr remove 1,369,896 + BBR_RS16140 0.59 -0.6 1,369,896 + BBR_RS16140 0.59 -0.6 1,369,897 + BBR_RS16140 0.59 -0.6 1,369,898 + BBR_RS16140 0.59 -0.3 1,370,029 - BBR_RS16140 0.65 +0.8 1,370,029 - BBR_RS16140 0.65 +0.4 1,370,088 + BBR_RS16140 0.68 -0.6 1,370,158 + BBR_RS16140 0.71 +4.3 1,370,177 + BBR_RS16140 0.72 +1.0 1,370,177 + BBR_RS16140 0.72 +1.0 1,370,185 + BBR_RS16140 0.73 +1.0 1,370,193 - BBR_RS16140 0.73 -3.1 1,370,233 - BBR_RS16140 0.75 +2.0 1,370,246 + BBR_RS16140 0.76 +1.0 1,370,286 + BBR_RS16140 0.78 -1.6 1,370,345 - BBR_RS16140 0.80 -1.0 1,370,350 - BBR_RS16140 0.81 +0.7 1,370,387 - BBR_RS16140 0.82 -0.9 1,370,390 + BBR_RS16140 0.83 +1.0 1,370,426 - BBR_RS16140 0.84 -1.3 1,370,426 - BBR_RS16140 0.84 +1.0 1,370,436 + BBR_RS16140 0.85 +2.6 1,370,526 - BBR_RS16140 0.89 +1.0 1,370,538 - BBR_RS16140 0.90 +1.0 1,370,538 - BBR_RS16140 0.90 +1.0 1,370,553 + -1.3 1,370,558 + +1.0 1,370,560 + -1.2 1,370,560 + -2.8 1,370,568 - +0.3 1,370,581 + -1.0 1,370,613 + +1.0 1,370,666 + +3.7 1,370,738 + +0.1 1,370,771 + -2.0 1,370,771 + +1.0 1,370,780 - -2.0 1,370,805 - +0.0 1,370,821 + +1.0 1,370,842 + -0.2 1,370,842 + -1.1 1,370,850 - +1.0 1,370,850 - -2.1 1,370,908 - BBR_RS16145 0.17 -0.6 1,370,916 - BBR_RS16145 0.19 -1.7 1,370,930 - BBR_RS16145 0.22 +1.0 1,370,988 + BBR_RS16145 0.34 +1.0 1,371,017 + BBR_RS16145 0.40 -1.3 1,371,028 - BBR_RS16145 0.42 +0.0 1,371,036 + BBR_RS16145 0.44 +1.0 1,371,044 - BBR_RS16145 0.45 -2.2 1,371,046 + BBR_RS16145 0.46 -2.0 1,371,054 - BBR_RS16145 0.47 +1.0 1,371,064 - BBR_RS16145 0.49 +0.0 1,371,067 + BBR_RS16145 0.50 -2.5 1,371,237 + BBR_RS16145 0.85 +0.0 1,371,263 - -1.4 1,371,263 - +3.8 1,371,279 - -1.8 1,371,360 + -0.9 1,371,368 - +0.9 1,371,409 + +1.2 1,371,458 - +1.0 1,371,609 - BBR_RS16150 0.14 -0.6 1,371,655 - BBR_RS16150 0.18 +0.4 1,371,660 - BBR_RS16150 0.18 -0.6 1,371,684 - BBR_RS16150 0.20 -0.6 1,371,700 - BBR_RS16150 0.22 -3.2 1,371,700 - BBR_RS16150 0.22 -0.6 1,371,804 + BBR_RS16150 0.31 +1.3 1,371,805 - BBR_RS16150 0.31 +1.0 1,371,812 - BBR_RS16150 0.32 -1.8 1,371,815 - BBR_RS16150 0.32 +2.6 1,371,866 - BBR_RS16150 0.36 -1.3 1,371,882 - BBR_RS16150 0.38 +1.0 1,371,893 - BBR_RS16150 0.39 -0.6 1,371,970 + BBR_RS16150 0.46 -1.0 1,371,983 + BBR_RS16150 0.47 -1.3 1,371,993 - BBR_RS16150 0.48 -0.7 1,371,993 - BBR_RS16150 0.48 +0.4 1,372,025 - BBR_RS16150 0.50 -0.3 1,372,064 + BBR_RS16150 0.54 -1.4 1,372,092 - BBR_RS16150 0.56 -1.0 1,372,094 - BBR_RS16150 0.57 -1.8 1,372,144 - BBR_RS16150 0.61 -1.0 1,372,144 - BBR_RS16150 0.61 +1.0 1,372,179 + BBR_RS16150 0.64 -1.0 1,372,224 + BBR_RS16150 0.68 -1.3 1,372,224 + BBR_RS16150 0.68 -1.1 1,372,257 + BBR_RS16150 0.71 +2.0
Or see this region's nucleotide sequence