Experiment: MRS + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13680 and BBR_RS13685 are separated by 357 nucleotides BBR_RS13685 and BBR_RS13690 are separated by 61 nucleotides
BBR_RS13680: BBR_RS13680 - SPFH domain-containing protein, at 843,446 to 844,357
_RS13680
BBR_RS13685: BBR_RS13685 - DUF4143 domain-containing protein, at 844,715 to 845,152
_RS13685
BBR_RS13690: BBR_RS13690 - patatin family protein, at 845,214 to 846,059
_RS13690
Position (kb)
844
845
846 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 843.722 kb on + strand, within BBR_RS13680 at 843.728 kb on - strand, within BBR_RS13680 at 843.728 kb on - strand, within BBR_RS13680 at 843.762 kb on + strand, within BBR_RS13680 at 843.770 kb on - strand, within BBR_RS13680 at 843.779 kb on - strand, within BBR_RS13680 at 843.796 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.838 kb on + strand, within BBR_RS13680 at 843.847 kb on - strand, within BBR_RS13680 at 843.851 kb on + strand, within BBR_RS13680 at 843.884 kb on + strand, within BBR_RS13680 at 843.892 kb on - strand, within BBR_RS13680 at 843.894 kb on - strand, within BBR_RS13680 at 843.929 kb on + strand, within BBR_RS13680 at 843.931 kb on + strand, within BBR_RS13680 at 843.939 kb on - strand, within BBR_RS13680 at 843.939 kb on - strand, within BBR_RS13680 at 843.970 kb on + strand, within BBR_RS13680 at 844.101 kb on + strand, within BBR_RS13680 at 844.107 kb on - strand, within BBR_RS13680 at 844.107 kb on - strand, within BBR_RS13680 at 844.146 kb on - strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.153 kb on + strand, within BBR_RS13680 at 844.156 kb on - strand, within BBR_RS13680 at 844.161 kb on - strand, within BBR_RS13680 at 844.168 kb on + strand, within BBR_RS13680 at 844.183 kb on + strand, within BBR_RS13680 at 844.191 kb on - strand, within BBR_RS13680 at 844.205 kb on + strand, within BBR_RS13680 at 844.208 kb on - strand, within BBR_RS13680 at 844.208 kb on - strand, within BBR_RS13680 at 844.216 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.258 kb on + strand, within BBR_RS13680 at 844.289 kb on - strand at 844.328 kb on + strand at 844.387 kb on + strand at 844.527 kb on + strand at 844.535 kb on - strand at 844.624 kb on + strand at 844.624 kb on + strand at 844.709 kb on + strand at 844.719 kb on - strand at 844.789 kb on + strand, within BBR_RS13685 at 844.803 kb on + strand, within BBR_RS13685 at 844.808 kb on + strand, within BBR_RS13685 at 844.817 kb on + strand, within BBR_RS13685 at 844.853 kb on + strand, within BBR_RS13685 at 844.861 kb on - strand, within BBR_RS13685 at 844.904 kb on + strand, within BBR_RS13685 at 844.910 kb on - strand, within BBR_RS13685 at 844.912 kb on - strand, within BBR_RS13685 at 844.914 kb on - strand, within BBR_RS13685 at 844.994 kb on + strand, within BBR_RS13685 at 844.994 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.018 kb on + strand, within BBR_RS13685 at 845.020 kb on - strand, within BBR_RS13685 at 845.026 kb on - strand, within BBR_RS13685 at 845.026 kb on - strand, within BBR_RS13685 at 845.069 kb on - strand, within BBR_RS13685 at 845.185 kb on + strand at 845.195 kb on + strand at 845.201 kb on - strand at 845.250 kb on + strand at 845.256 kb on + strand at 845.257 kb on - strand at 845.265 kb on - strand at 845.304 kb on + strand, within BBR_RS13690 at 845.311 kb on + strand, within BBR_RS13690 at 845.311 kb on + strand, within BBR_RS13690 at 845.319 kb on - strand, within BBR_RS13690 at 845.369 kb on - strand, within BBR_RS13690 at 845.392 kb on + strand, within BBR_RS13690 at 845.400 kb on - strand, within BBR_RS13690 at 845.400 kb on - strand, within BBR_RS13690 at 845.436 kb on - strand, within BBR_RS13690 at 845.444 kb on - strand, within BBR_RS13690 at 845.524 kb on - strand, within BBR_RS13690 at 845.540 kb on - strand, within BBR_RS13690 at 845.540 kb on - strand, within BBR_RS13690 at 845.540 kb on - strand, within BBR_RS13690 at 845.547 kb on + strand, within BBR_RS13690 at 845.549 kb on - strand, within BBR_RS13690 at 845.592 kb on + strand, within BBR_RS13690 at 845.592 kb on + strand, within BBR_RS13690 at 845.607 kb on - strand, within BBR_RS13690 at 845.705 kb on - strand, within BBR_RS13690 at 845.787 kb on - strand, within BBR_RS13690 at 845.808 kb on - strand, within BBR_RS13690 at 845.808 kb on - strand, within BBR_RS13690 at 845.818 kb on - strand, within BBR_RS13690 at 845.865 kb on - strand, within BBR_RS13690 at 845.950 kb on + strand, within BBR_RS13690 at 846.006 kb on + strand at 846.006 kb on + strand at 846.030 kb on + strand at 846.117 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time48hr remove 843,722 + BBR_RS13680 0.30 -0.1 843,728 - BBR_RS13680 0.31 +0.9 843,728 - BBR_RS13680 0.31 +2.2 843,762 + BBR_RS13680 0.35 +2.5 843,770 - BBR_RS13680 0.36 +1.2 843,779 - BBR_RS13680 0.37 -0.6 843,796 + BBR_RS13680 0.38 -0.5 843,838 + BBR_RS13680 0.43 +0.4 843,838 + BBR_RS13680 0.43 -0.4 843,838 + BBR_RS13680 0.43 -0.6 843,847 - BBR_RS13680 0.44 +0.9 843,851 + BBR_RS13680 0.44 -0.4 843,884 + BBR_RS13680 0.48 +0.9 843,892 - BBR_RS13680 0.49 -0.1 843,894 - BBR_RS13680 0.49 -0.1 843,929 + BBR_RS13680 0.53 +3.3 843,931 + BBR_RS13680 0.53 -1.1 843,939 - BBR_RS13680 0.54 -1.6 843,939 - BBR_RS13680 0.54 +0.9 843,970 + BBR_RS13680 0.57 -3.0 844,101 + BBR_RS13680 0.72 +1.9 844,107 - BBR_RS13680 0.72 +0.4 844,107 - BBR_RS13680 0.72 -1.4 844,146 - BBR_RS13680 0.77 +1.2 844,153 + BBR_RS13680 0.78 +0.9 844,153 + BBR_RS13680 0.78 -2.1 844,153 + BBR_RS13680 0.78 +0.9 844,153 + BBR_RS13680 0.78 -0.9 844,153 + BBR_RS13680 0.78 +0.9 844,156 - BBR_RS13680 0.78 +1.9 844,161 - BBR_RS13680 0.78 -0.4 844,168 + BBR_RS13680 0.79 -2.1 844,183 + BBR_RS13680 0.81 +0.9 844,191 - BBR_RS13680 0.82 -0.1 844,205 + BBR_RS13680 0.83 -1.4 844,208 - BBR_RS13680 0.84 -1.6 844,208 - BBR_RS13680 0.84 -2.5 844,216 + BBR_RS13680 0.84 +0.9 844,258 + BBR_RS13680 0.89 -2.6 844,258 + BBR_RS13680 0.89 -0.9 844,258 + BBR_RS13680 0.89 +1.5 844,289 - +1.8 844,328 + +0.6 844,387 + -0.1 844,527 + -0.1 844,535 - -0.6 844,624 + -1.4 844,624 + +1.9 844,709 + +0.6 844,719 - -1.1 844,789 + BBR_RS13685 0.17 +0.9 844,803 + BBR_RS13685 0.20 +2.3 844,808 + BBR_RS13685 0.21 +0.9 844,817 + BBR_RS13685 0.23 +0.9 844,853 + BBR_RS13685 0.32 -1.6 844,861 - BBR_RS13685 0.33 -0.3 844,904 + BBR_RS13685 0.43 +3.9 844,910 - BBR_RS13685 0.45 +0.9 844,912 - BBR_RS13685 0.45 -1.4 844,914 - BBR_RS13685 0.45 +0.3 844,994 + BBR_RS13685 0.64 +0.4 844,994 + BBR_RS13685 0.64 -1.9 845,018 + BBR_RS13685 0.69 +3.3 845,018 + BBR_RS13685 0.69 +0.9 845,018 + BBR_RS13685 0.69 -0.1 845,018 + BBR_RS13685 0.69 +0.9 845,018 + BBR_RS13685 0.69 +1.5 845,018 + BBR_RS13685 0.69 +2.5 845,018 + BBR_RS13685 0.69 +0.9 845,020 - BBR_RS13685 0.70 +0.5 845,026 - BBR_RS13685 0.71 +1.9 845,026 - BBR_RS13685 0.71 -0.1 845,069 - BBR_RS13685 0.81 +1.4 845,185 + -0.1 845,195 + -0.9 845,201 - +0.9 845,250 + +3.3 845,256 + +0.9 845,257 - -2.2 845,265 - -1.7 845,304 + BBR_RS13690 0.11 -1.1 845,311 + BBR_RS13690 0.11 -2.2 845,311 + BBR_RS13690 0.11 +2.2 845,319 - BBR_RS13690 0.12 -0.1 845,369 - BBR_RS13690 0.18 +2.9 845,392 + BBR_RS13690 0.21 +0.9 845,400 - BBR_RS13690 0.22 -1.7 845,400 - BBR_RS13690 0.22 +0.1 845,436 - BBR_RS13690 0.26 -2.1 845,444 - BBR_RS13690 0.27 -1.9 845,524 - BBR_RS13690 0.37 -2.2 845,540 - BBR_RS13690 0.39 +1.7 845,540 - BBR_RS13690 0.39 -0.6 845,540 - BBR_RS13690 0.39 -3.9 845,547 + BBR_RS13690 0.39 -2.5 845,549 - BBR_RS13690 0.40 -1.1 845,592 + BBR_RS13690 0.45 -3.1 845,592 + BBR_RS13690 0.45 -1.9 845,607 - BBR_RS13690 0.46 -0.7 845,705 - BBR_RS13690 0.58 -1.4 845,787 - BBR_RS13690 0.68 -2.4 845,808 - BBR_RS13690 0.70 +4.5 845,808 - BBR_RS13690 0.70 +0.6 845,818 - BBR_RS13690 0.71 -1.4 845,865 - BBR_RS13690 0.77 +1.9 845,950 + BBR_RS13690 0.87 +0.9 846,006 + -1.5 846,006 + +0.9 846,030 + -1.1 846,117 + +1.1
Or see this region's nucleotide sequence