Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0399 and Psyr_0400 are separated by 13 nucleotides Psyr_0400 and Psyr_0401 are separated by 181 nucleotides Psyr_0401 and Psyr_0402 are separated by 584 nucleotides
Psyr_0399: Psyr_0399 - LSU ribosomal protein L31P, at 427,900 to 428,121
_0399
Psyr_0400: Psyr_0400 - nuclease (SNase-like), at 428,135 to 428,938
_0400
Psyr_0401: Psyr_0401 - Malate dehydrogenase (oxaloacetate decarboxylating) (NADP+), at 429,120 to 430,388
_0401
Psyr_0402: Psyr_0402 - Peptidoglycan glycosyltransferase, at 430,973 to 433,417
_0402
Position (kb) 429
430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 428.120 kb on - strand at 428.120 kb on - strand at 428.120 kb on - strand at 428.283 kb on + strand, within Psyr_0400 at 428.326 kb on + strand, within Psyr_0400 at 428.327 kb on - strand, within Psyr_0400 at 428.459 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400 at 428.936 kb on + strand at 428.987 kb on + strand at 428.999 kb on - strand at 428.999 kb on - strand at 429.003 kb on + strand at 429.003 kb on + strand at 429.003 kb on + strand at 429.004 kb on - strand at 429.004 kb on - strand at 429.010 kb on + strand at 429.010 kb on + strand at 429.010 kb on + strand at 429.011 kb on - strand at 429.022 kb on + strand at 429.023 kb on - strand at 429.051 kb on + strand at 429.051 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.070 kb on + strand at 429.098 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.151 kb on - strand at 429.151 kb on - strand at 429.231 kb on + strand at 429.232 kb on - strand at 429.232 kb on - strand at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.316 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.491 kb on + strand, within Psyr_0401 at 429.780 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 430.051 kb on - strand, within Psyr_0401 at 430.210 kb on - strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.242 kb on + strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.243 kb on - strand, within Psyr_0401 at 430.547 kb on - strand at 430.547 kb on - strand at 430.578 kb on - strand at 430.578 kb on - strand at 430.579 kb on + strand at 430.579 kb on + strand at 430.579 kb on + strand at 430.579 kb on + strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.580 kb on - strand at 430.795 kb on + strand at 430.797 kb on + strand at 430.797 kb on + strand at 430.797 kb on + strand at 430.797 kb on + strand at 430.798 kb on - strand at 430.798 kb on - strand at 430.854 kb on + strand at 430.854 kb on + strand at 430.854 kb on + strand at 430.854 kb on + strand at 430.855 kb on - strand at 430.855 kb on - strand at 430.855 kb on - strand at 430.971 kb on + strand at 431.208 kb on + strand at 431.208 kb on + strand at 431.208 kb on + strand at 431.208 kb on + strand at 431.208 kb on + strand at 431.208 kb on + strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.209 kb on - strand at 431.301 kb on + strand, within Psyr_0402 at 431.301 kb on + strand, within Psyr_0402 at 431.301 kb on + strand, within Psyr_0402 at 431.301 kb on + strand, within Psyr_0402 at 431.313 kb on + strand, within Psyr_0402 at 431.314 kb on - strand, within Psyr_0402
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 428,120 - +0.2 428,120 - +0.0 428,120 - +0.8 428,283 + Psyr_0400 0.18 -1.1 428,326 + Psyr_0400 0.24 -0.2 428,327 - Psyr_0400 0.24 -0.2 428,459 + Psyr_0400 0.40 -0.3 428,483 + Psyr_0400 0.43 +0.3 428,483 + Psyr_0400 0.43 -0.2 428,484 - Psyr_0400 0.43 -0.9 428,484 - Psyr_0400 0.43 -1.2 428,484 - Psyr_0400 0.43 -0.4 428,537 + Psyr_0400 0.50 -2.0 428,537 + Psyr_0400 0.50 +0.1 428,936 + +0.3 428,987 + +0.2 428,999 - +0.7 428,999 - -2.1 429,003 + +1.4 429,003 + -0.1 429,003 + -0.4 429,004 - -0.3 429,004 - -0.6 429,010 + -0.5 429,010 + -0.2 429,010 + -1.3 429,011 - -0.5 429,022 + -0.1 429,023 - -0.6 429,051 + -1.0 429,051 + -0.8 429,057 + -0.2 429,057 + -2.1 429,057 + -0.4 429,057 + -0.1 429,057 + +0.5 429,058 - +0.4 429,058 - -0.4 429,058 - -0.4 429,058 - +0.2 429,070 + -0.5 429,098 + -0.6 429,117 + -1.3 429,117 + +1.1 429,117 + -2.0 429,117 + -0.1 429,118 - +0.4 429,118 - -0.1 429,118 - -0.3 429,118 - +1.1 429,118 - -0.3 429,118 - -0.8 429,118 - -0.6 429,118 - -0.0 429,118 - +0.8 429,118 - +0.2 429,151 - -1.1 429,151 - -0.0 429,231 + +0.0 429,232 - +0.4 429,232 - -0.6 429,288 + Psyr_0401 0.13 -0.6 429,288 + Psyr_0401 0.13 +0.2 429,288 + Psyr_0401 0.13 +0.0 429,288 + Psyr_0401 0.13 -1.2 429,289 - Psyr_0401 0.13 +0.3 429,289 - Psyr_0401 0.13 -1.1 429,289 - Psyr_0401 0.13 -0.6 429,294 + Psyr_0401 0.14 -0.4 429,294 + Psyr_0401 0.14 -1.0 429,294 + Psyr_0401 0.14 -0.3 429,294 + Psyr_0401 0.14 +1.0 429,294 + Psyr_0401 0.14 -1.0 429,295 - Psyr_0401 0.14 -0.1 429,295 - Psyr_0401 0.14 -0.4 429,295 - Psyr_0401 0.14 -0.1 429,295 - Psyr_0401 0.14 -1.0 429,295 - Psyr_0401 0.14 +0.4 429,295 - Psyr_0401 0.14 -0.6 429,295 - Psyr_0401 0.14 -0.2 429,295 - Psyr_0401 0.14 -0.9 429,316 + Psyr_0401 0.15 -0.4 429,323 + Psyr_0401 0.16 -1.4 429,323 + Psyr_0401 0.16 -0.2 429,323 + Psyr_0401 0.16 -1.2 429,324 - Psyr_0401 0.16 +1.7 429,324 - Psyr_0401 0.16 +0.2 429,324 - Psyr_0401 0.16 +0.6 429,395 + Psyr_0401 0.22 -0.2 429,395 + Psyr_0401 0.22 +0.6 429,395 + Psyr_0401 0.22 +0.5 429,491 + Psyr_0401 0.29 -0.2 429,780 - Psyr_0401 0.52 +0.8 429,820 - Psyr_0401 0.55 +0.5 429,820 - Psyr_0401 0.55 -0.6 429,886 + Psyr_0401 0.60 +0.1 429,886 + Psyr_0401 0.60 +0.1 429,887 - Psyr_0401 0.60 -0.5 429,887 - Psyr_0401 0.60 +1.1 429,887 - Psyr_0401 0.60 -0.5 430,051 - Psyr_0401 0.73 -0.3 430,210 - Psyr_0401 0.86 -1.5 430,242 + Psyr_0401 0.88 +0.8 430,242 + Psyr_0401 0.88 -1.3 430,242 + Psyr_0401 0.88 -0.9 430,242 + Psyr_0401 0.88 +0.7 430,242 + Psyr_0401 0.88 -1.4 430,242 + Psyr_0401 0.88 -0.4 430,242 + Psyr_0401 0.88 -2.6 430,242 + Psyr_0401 0.88 +0.1 430,242 + Psyr_0401 0.88 -0.1 430,242 + Psyr_0401 0.88 +0.3 430,243 - Psyr_0401 0.88 -0.4 430,243 - Psyr_0401 0.88 +0.0 430,243 - Psyr_0401 0.88 -0.5 430,243 - Psyr_0401 0.88 -0.4 430,243 - Psyr_0401 0.88 -0.9 430,243 - Psyr_0401 0.88 +1.2 430,243 - Psyr_0401 0.88 -0.8 430,547 - -0.2 430,547 - +0.4 430,578 - -1.2 430,578 - -0.6 430,579 + -0.2 430,579 + +1.2 430,579 + -0.3 430,579 + +0.4 430,580 - -0.8 430,580 - +1.0 430,580 - -0.4 430,580 - +0.4 430,580 - +0.1 430,580 - -0.5 430,580 - -0.7 430,580 - +1.0 430,795 + -0.2 430,797 + -1.8 430,797 + +0.4 430,797 + +0.6 430,797 + -1.1 430,798 - +0.3 430,798 - -0.9 430,854 + +0.4 430,854 + +0.2 430,854 + -1.0 430,854 + -0.7 430,855 - +2.1 430,855 - -0.5 430,855 - -0.6 430,971 + -0.2 431,208 + -2.2 431,208 + -2.8 431,208 + -0.2 431,208 + -3.5 431,208 + +0.4 431,208 + -1.1 431,209 - -1.3 431,209 - -1.7 431,209 - -1.9 431,209 - -2.4 431,209 - -0.6 431,209 - -0.6 431,209 - -2.1 431,209 - +0.2 431,301 + Psyr_0402 0.13 -1.0 431,301 + Psyr_0402 0.13 -0.8 431,301 + Psyr_0402 0.13 -1.3 431,301 + Psyr_0402 0.13 -2.0 431,313 + Psyr_0402 0.14 -1.4 431,314 - Psyr_0402 0.14 -1.0
Or see this region's nucleotide sequence