Experiment: library passed through aphids in food, 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0232 and Psyr_0233 are separated by 105 nucleotides Psyr_0233 and Psyr_0234 are separated by 7 nucleotides Psyr_0234 and Psyr_0235 are separated by 157 nucleotides
Psyr_0232: Psyr_0232 - gamma-glutamylputrescine oxidase, at 246,686 to 247,999
_0232
Psyr_0233: Psyr_0233 - Protein of unknown function UPF0153, at 248,105 to 248,458
_0233
Psyr_0234: Psyr_0234 - transcriptional regulator, AsnC family, at 248,466 to 248,954
_0234
Psyr_0235: Psyr_0235 - D-amino acid dehydrogenase small subunit, at 249,112 to 250,413
_0235
Position (kb) 248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 247.107 kb on + strand, within Psyr_0232 at 247.107 kb on + strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.111 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.257 kb on + strand, within Psyr_0232 at 247.257 kb on + strand, within Psyr_0232 at 247.310 kb on - strand, within Psyr_0232 at 247.461 kb on + strand, within Psyr_0232 at 247.944 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.947 kb on + strand at 247.948 kb on - strand at 247.948 kb on - strand at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.448 kb on + strand at 248.448 kb on + strand at 248.449 kb on - strand at 248.449 kb on - strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.457 kb on - strand at 248.457 kb on - strand at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.614 kb on + strand, within Psyr_0234 at 248.615 kb on - strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.835 kb on - strand, within Psyr_0234 at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 249.018 kb on - strand at 249.048 kb on + strand at 249.048 kb on + strand at 249.049 kb on - strand at 249.147 kb on + strand at 249.160 kb on + strand at 249.160 kb on + strand at 249.161 kb on - strand at 249.161 kb on - strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.164 kb on - strand at 249.262 kb on + strand, within Psyr_0235 at 249.356 kb on - strand, within Psyr_0235 at 249.358 kb on - strand, within Psyr_0235 at 249.367 kb on + strand, within Psyr_0235 at 249.368 kb on - strand, within Psyr_0235 at 249.412 kb on + strand, within Psyr_0235
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 24 hours remove 247,107 + Psyr_0232 0.32 +1.3 247,107 + Psyr_0232 0.32 +0.9 247,108 - Psyr_0232 0.32 -0.0 247,108 - Psyr_0232 0.32 +0.3 247,110 + Psyr_0232 0.32 -1.2 247,110 + Psyr_0232 0.32 -0.4 247,111 - Psyr_0232 0.32 +1.1 247,137 - Psyr_0232 0.34 -1.1 247,137 - Psyr_0232 0.34 +0.4 247,203 + Psyr_0232 0.39 +0.1 247,203 + Psyr_0232 0.39 +0.3 247,203 + Psyr_0232 0.39 -0.8 247,203 + Psyr_0232 0.39 +0.1 247,203 + Psyr_0232 0.39 +0.2 247,204 - Psyr_0232 0.39 +0.5 247,204 - Psyr_0232 0.39 +0.2 247,204 - Psyr_0232 0.39 +0.0 247,204 - Psyr_0232 0.39 +0.8 247,204 - Psyr_0232 0.39 -0.9 247,204 - Psyr_0232 0.39 +0.3 247,204 - Psyr_0232 0.39 +0.2 247,204 - Psyr_0232 0.39 -2.8 247,204 - Psyr_0232 0.39 +1.2 247,257 + Psyr_0232 0.43 +0.0 247,257 + Psyr_0232 0.43 +0.2 247,310 - Psyr_0232 0.47 -0.9 247,461 + Psyr_0232 0.59 +0.6 247,944 + +0.6 247,947 + +0.7 247,947 + -1.5 247,947 + -0.7 247,947 + -0.1 247,947 + +0.2 247,948 - -0.1 247,948 - -0.3 248,280 + Psyr_0233 0.49 +0.7 248,280 + Psyr_0233 0.49 -1.7 248,280 + Psyr_0233 0.49 -0.4 248,280 + Psyr_0233 0.49 +0.0 248,281 - Psyr_0233 0.50 +0.3 248,281 - Psyr_0233 0.50 +0.5 248,281 - Psyr_0233 0.50 +0.4 248,309 + Psyr_0233 0.58 -0.9 248,309 + Psyr_0233 0.58 -0.6 248,309 + Psyr_0233 0.58 -1.6 248,310 - Psyr_0233 0.58 -0.8 248,310 - Psyr_0233 0.58 -0.5 248,310 - Psyr_0233 0.58 +0.6 248,418 + Psyr_0233 0.88 -1.2 248,418 + Psyr_0233 0.88 -0.4 248,418 + Psyr_0233 0.88 -0.1 248,419 - Psyr_0233 0.89 -0.8 248,419 - Psyr_0233 0.89 -0.2 248,426 + -0.6 248,426 + -0.1 248,426 + -1.2 248,426 + +0.2 248,426 + +0.1 248,426 + +0.3 248,426 + -0.2 248,426 + -0.9 248,426 + +0.7 248,426 + -0.5 248,426 + -0.6 248,426 + +1.9 248,426 + -0.2 248,426 + +1.0 248,427 - -0.0 248,427 - -0.0 248,427 - +0.3 248,427 - -0.5 248,427 - +0.1 248,427 - +1.2 248,427 - -2.0 248,427 - +2.1 248,427 - +0.0 248,427 - +0.2 248,427 - -0.4 248,427 - +0.7 248,427 - -2.2 248,427 - -0.3 248,428 + -0.9 248,428 + +0.4 248,428 + -1.3 248,428 + +1.3 248,428 + -0.6 248,428 + -0.4 248,428 + -0.9 248,428 + -0.3 248,429 - +0.0 248,429 - -0.8 248,429 - -0.9 248,429 - -0.6 248,429 - -0.2 248,429 - +0.6 248,429 - -0.1 248,429 - -0.4 248,429 - +0.8 248,448 + -0.3 248,448 + -0.1 248,449 - +1.2 248,449 - -0.0 248,454 + -0.1 248,454 + -0.1 248,454 + +0.3 248,454 + +0.6 248,454 + -1.3 248,454 + +0.4 248,454 + -0.5 248,455 - -0.3 248,455 - +1.8 248,455 - +0.1 248,455 - +0.6 248,455 - +0.7 248,455 - +0.1 248,456 + +0.2 248,456 + -0.8 248,456 + -1.6 248,456 + +0.2 248,456 + -1.0 248,457 - -0.1 248,457 - -0.7 248,518 + Psyr_0234 0.11 -0.7 248,518 + Psyr_0234 0.11 -0.5 248,518 + Psyr_0234 0.11 -2.2 248,518 + Psyr_0234 0.11 +0.6 248,518 + Psyr_0234 0.11 +0.4 248,518 + Psyr_0234 0.11 -0.6 248,518 + Psyr_0234 0.11 -2.4 248,518 + Psyr_0234 0.11 -1.9 248,518 + Psyr_0234 0.11 -0.3 248,518 + Psyr_0234 0.11 +0.8 248,518 + Psyr_0234 0.11 -0.2 248,518 + Psyr_0234 0.11 -0.6 248,519 - Psyr_0234 0.11 -0.1 248,519 - Psyr_0234 0.11 -0.4 248,519 - Psyr_0234 0.11 -0.3 248,519 - Psyr_0234 0.11 +0.4 248,519 - Psyr_0234 0.11 -1.2 248,519 - Psyr_0234 0.11 +0.4 248,519 - Psyr_0234 0.11 -1.4 248,519 - Psyr_0234 0.11 -0.6 248,519 - Psyr_0234 0.11 -1.2 248,519 - Psyr_0234 0.11 +0.6 248,519 - Psyr_0234 0.11 -0.8 248,519 - Psyr_0234 0.11 -0.8 248,614 + Psyr_0234 0.30 +1.0 248,615 - Psyr_0234 0.30 -0.2 248,707 + Psyr_0234 0.49 +0.2 248,707 + Psyr_0234 0.49 -1.0 248,707 + Psyr_0234 0.49 +0.3 248,707 + Psyr_0234 0.49 +0.6 248,707 + Psyr_0234 0.49 +0.8 248,708 - Psyr_0234 0.49 +0.4 248,708 - Psyr_0234 0.49 +1.3 248,708 - Psyr_0234 0.49 -0.9 248,708 - Psyr_0234 0.49 -1.6 248,708 - Psyr_0234 0.49 -1.3 248,835 - Psyr_0234 0.75 -1.4 248,913 + +0.4 248,913 + -0.5 248,913 + +0.5 248,913 + -1.4 248,913 + -0.4 248,913 + -0.4 248,913 + -0.2 248,913 + +0.5 248,913 + -1.4 248,913 + +0.7 248,913 + +0.6 248,914 - +0.1 248,914 - -0.2 248,914 - +0.8 248,914 - +0.4 248,914 - -0.3 248,914 - -0.7 248,914 - +0.6 248,914 - -1.0 248,914 - +0.1 248,914 - -0.9 249,018 - -0.8 249,048 + -0.6 249,048 + +0.1 249,049 - +0.2 249,147 + -1.0 249,160 + +0.3 249,160 + -0.4 249,161 - -1.2 249,161 - -0.9 249,163 + -1.0 249,163 + +0.6 249,163 + -0.8 249,163 + -0.1 249,163 + -0.9 249,164 - -0.5 249,262 + Psyr_0235 0.12 -0.8 249,356 - Psyr_0235 0.19 +0.1 249,358 - Psyr_0235 0.19 -0.2 249,367 + Psyr_0235 0.20 -0.2 249,368 - Psyr_0235 0.20 -0.5 249,412 + Psyr_0235 0.23 -1.2
Or see this region's nucleotide sequence