Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1401 and Pf6N2E2_1402 are separated by 15 nucleotides Pf6N2E2_1402 and Pf6N2E2_1403 are separated by 119 nucleotides Pf6N2E2_1403 and Pf6N2E2_1404 are separated by 30 nucleotides Pf6N2E2_1404 and Pf6N2E2_1405 overlap by 4 nucleotides Pf6N2E2_1405 and Pf6N2E2_1406 overlap by 4 nucleotides
Pf6N2E2_1401 - FIG00955631: hypothetical protein, at 1,642,203 to 1,643,366
_1401
Pf6N2E2_1402 - hypothetical protein, at 1,643,382 to 1,643,579
_1402
Pf6N2E2_1403 - Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL, at 1,643,699 to 1,644,226
_1403
Pf6N2E2_1404 - Nitrous oxide reductase maturation transmembrane protein NosY, at 1,644,257 to 1,645,087
_1404
Pf6N2E2_1405 - Nitrous oxide reductase maturation protein NosF (ATPase), at 1,645,084 to 1,646,001
_1405
Pf6N2E2_1406 - Nitrous oxide reductase maturation protein NosD, at 1,645,998 to 1,647,311
_1406
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1643.406 kb on - strand, within Pf6N2E2_1402 at 1643.406 kb on - strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.428 kb on + strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.429 kb on - strand, within Pf6N2E2_1402 at 1643.433 kb on + strand, within Pf6N2E2_1402 at 1643.433 kb on + strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.434 kb on - strand, within Pf6N2E2_1402 at 1643.520 kb on + strand, within Pf6N2E2_1402 at 1643.521 kb on - strand, within Pf6N2E2_1402 at 1643.874 kb on + strand, within Pf6N2E2_1403 at 1643.946 kb on + strand, within Pf6N2E2_1403 at 1643.946 kb on + strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1643.947 kb on - strand, within Pf6N2E2_1403 at 1644.213 kb on + strand at 1644.213 kb on + strand at 1644.214 kb on - strand at 1644.214 kb on - strand at 1644.214 kb on - strand at 1644.314 kb on + strand at 1644.315 kb on - strand at 1644.603 kb on + strand, within Pf6N2E2_1404 at 1644.603 kb on + strand, within Pf6N2E2_1404 at 1644.604 kb on - strand, within Pf6N2E2_1404 at 1644.604 kb on - strand, within Pf6N2E2_1404 at 1644.810 kb on + strand, within Pf6N2E2_1404 at 1644.865 kb on - strand, within Pf6N2E2_1404 at 1645.087 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.121 kb on + strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.122 kb on - strand at 1645.206 kb on + strand, within Pf6N2E2_1405 at 1645.207 kb on - strand, within Pf6N2E2_1405 at 1645.499 kb on + strand, within Pf6N2E2_1405 at 1645.499 kb on + strand, within Pf6N2E2_1405 at 1645.500 kb on - strand, within Pf6N2E2_1405 at 1645.616 kb on + strand, within Pf6N2E2_1405 at 1645.617 kb on - strand, within Pf6N2E2_1405 at 1645.617 kb on - strand, within Pf6N2E2_1405 at 1645.745 kb on + strand, within Pf6N2E2_1405 at 1645.745 kb on + strand, within Pf6N2E2_1405 at 1645.770 kb on - strand at 1645.965 kb on - strand at 1645.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 1,643,406 - Pf6N2E2_1402 0.12 -1.9 1,643,406 - Pf6N2E2_1402 0.12 -1.4 1,643,428 + Pf6N2E2_1402 0.23 -1.7 1,643,428 + Pf6N2E2_1402 0.23 -2.7 1,643,428 + Pf6N2E2_1402 0.23 -1.2 1,643,428 + Pf6N2E2_1402 0.23 -3.3 1,643,428 + Pf6N2E2_1402 0.23 -0.9 1,643,429 - Pf6N2E2_1402 0.24 -1.8 1,643,429 - Pf6N2E2_1402 0.24 -0.9 1,643,429 - Pf6N2E2_1402 0.24 -2.4 1,643,429 - Pf6N2E2_1402 0.24 -1.7 1,643,429 - Pf6N2E2_1402 0.24 -1.7 1,643,433 + Pf6N2E2_1402 0.26 -3.1 1,643,433 + Pf6N2E2_1402 0.26 -1.7 1,643,434 - Pf6N2E2_1402 0.26 -2.9 1,643,434 - Pf6N2E2_1402 0.26 -3.3 1,643,434 - Pf6N2E2_1402 0.26 -3.6 1,643,434 - Pf6N2E2_1402 0.26 -3.8 1,643,520 + Pf6N2E2_1402 0.70 -1.1 1,643,521 - Pf6N2E2_1402 0.70 -1.2 1,643,874 + Pf6N2E2_1403 0.33 -0.0 1,643,946 + Pf6N2E2_1403 0.47 +0.1 1,643,946 + Pf6N2E2_1403 0.47 -0.9 1,643,947 - Pf6N2E2_1403 0.47 -1.7 1,643,947 - Pf6N2E2_1403 0.47 -0.9 1,643,947 - Pf6N2E2_1403 0.47 -1.5 1,644,213 + -0.2 1,644,213 + +0.2 1,644,214 - -0.3 1,644,214 - -0.1 1,644,214 - +0.3 1,644,314 + +0.9 1,644,315 - +0.6 1,644,603 + Pf6N2E2_1404 0.42 -1.9 1,644,603 + Pf6N2E2_1404 0.42 -0.3 1,644,604 - Pf6N2E2_1404 0.42 -3.5 1,644,604 - Pf6N2E2_1404 0.42 -1.9 1,644,810 + Pf6N2E2_1404 0.67 -0.1 1,644,865 - Pf6N2E2_1404 0.73 -2.5 1,645,087 + +0.7 1,645,121 + +0.4 1,645,121 + +1.0 1,645,121 + -0.3 1,645,121 + +0.4 1,645,121 + +0.1 1,645,121 + +0.1 1,645,121 + -1.1 1,645,121 + -0.7 1,645,122 - -1.1 1,645,122 - -0.5 1,645,122 - -1.5 1,645,122 - +0.4 1,645,122 - -1.0 1,645,122 - +1.0 1,645,122 - -0.1 1,645,206 + Pf6N2E2_1405 0.13 -0.7 1,645,207 - Pf6N2E2_1405 0.13 -0.3 1,645,499 + Pf6N2E2_1405 0.45 -0.1 1,645,499 + Pf6N2E2_1405 0.45 +0.9 1,645,500 - Pf6N2E2_1405 0.45 +0.0 1,645,616 + Pf6N2E2_1405 0.58 +1.2 1,645,617 - Pf6N2E2_1405 0.58 +0.9 1,645,617 - Pf6N2E2_1405 0.58 +0.3 1,645,745 + Pf6N2E2_1405 0.72 -0.5 1,645,745 + Pf6N2E2_1405 0.72 -0.5 1,645,770 - +1.7 1,645,965 - -0.1 1,645,965 - +0.1
Or see this region's nucleotide sequence