Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3824 and Dshi_3825 are separated by 22 nucleotides Dshi_3825 and Dshi_3826 are separated by 212 nucleotides Dshi_3826 and Dshi_3827 are separated by 41 nucleotides
Dshi_3824: Dshi_3824 - phenylacetate-CoA ligase (RefSeq), at 30,459 to 31,769
_3824
Dshi_3825: Dshi_3825 - phenylacetic acid degradation protein PaaD (RefSeq), at 31,792 to 32,217
_3825
Dshi_3826: Dshi_3826 - 3-hydroxyacyl-CoA dehydrogenase NAD-binding (RefSeq), at 32,430 to 34,451
_3826
Dshi_3827: Dshi_3827 - phenylacetic acid degradation protein paaN (RefSeq), at 34,493 to 36,529
_3827
Position (kb)
32
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 31.446 kb on + strand, within Dshi_3824 at 31.471 kb on - strand, within Dshi_3824 at 31.482 kb on + strand, within Dshi_3824 at 31.501 kb on - strand, within Dshi_3824 at 31.698 kb on - strand at 31.747 kb on - strand at 31.773 kb on + strand at 31.784 kb on + strand at 31.810 kb on + strand at 31.858 kb on + strand, within Dshi_3825 at 31.944 kb on + strand, within Dshi_3825 at 31.995 kb on + strand, within Dshi_3825 at 32.083 kb on - strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.094 kb on + strand, within Dshi_3825 at 32.102 kb on - strand, within Dshi_3825 at 32.148 kb on + strand, within Dshi_3825 at 32.308 kb on + strand at 32.704 kb on + strand, within Dshi_3826 at 32.721 kb on + strand, within Dshi_3826 at 33.061 kb on + strand, within Dshi_3826 at 33.124 kb on - strand, within Dshi_3826 at 33.181 kb on - strand, within Dshi_3826 at 33.257 kb on + strand, within Dshi_3826 at 33.291 kb on + strand, within Dshi_3826 at 33.342 kb on - strand, within Dshi_3826 at 33.345 kb on + strand, within Dshi_3826 at 33.472 kb on + strand, within Dshi_3826 at 33.477 kb on + strand, within Dshi_3826 at 33.478 kb on - strand, within Dshi_3826 at 33.515 kb on + strand, within Dshi_3826 at 33.666 kb on + strand, within Dshi_3826 at 33.994 kb on - strand, within Dshi_3826 at 34.047 kb on + strand, within Dshi_3826 at 34.196 kb on - strand, within Dshi_3826 at 34.212 kb on + strand, within Dshi_3826 at 34.212 kb on + strand, within Dshi_3826 at 34.354 kb on + strand at 34.491 kb on + strand at 34.535 kb on - strand at 34.656 kb on + strand at 34.712 kb on + strand, within Dshi_3827 at 34.756 kb on - strand, within Dshi_3827 at 34.817 kb on + strand, within Dshi_3827 at 34.817 kb on + strand, within Dshi_3827 at 34.825 kb on - strand, within Dshi_3827 at 34.825 kb on - strand, within Dshi_3827 at 34.929 kb on - strand, within Dshi_3827 at 34.986 kb on - strand, within Dshi_3827 at 35.169 kb on + strand, within Dshi_3827 at 35.169 kb on + strand, within Dshi_3827 at 35.306 kb on + strand, within Dshi_3827
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 31,446 + Dshi_3824 0.75 +0.4 31,471 - Dshi_3824 0.77 +0.5 31,482 + Dshi_3824 0.78 -0.2 31,501 - Dshi_3824 0.79 +2.7 31,698 - +0.9 31,747 - -0.1 31,773 + -0.3 31,784 + -0.5 31,810 + -2.5 31,858 + Dshi_3825 0.15 +2.2 31,944 + Dshi_3825 0.36 +0.2 31,995 + Dshi_3825 0.48 +1.5 32,083 - Dshi_3825 0.68 +0.3 32,094 + Dshi_3825 0.71 +0.1 32,094 + Dshi_3825 0.71 +0.4 32,094 + Dshi_3825 0.71 -1.6 32,102 - Dshi_3825 0.73 +1.2 32,148 + Dshi_3825 0.84 +1.0 32,308 + +0.4 32,704 + Dshi_3826 0.14 +1.7 32,721 + Dshi_3826 0.14 -0.1 33,061 + Dshi_3826 0.31 +0.0 33,124 - Dshi_3826 0.34 -1.6 33,181 - Dshi_3826 0.37 -1.9 33,257 + Dshi_3826 0.41 +0.9 33,291 + Dshi_3826 0.43 +0.5 33,342 - Dshi_3826 0.45 +0.1 33,345 + Dshi_3826 0.45 -0.5 33,472 + Dshi_3826 0.52 -1.2 33,477 + Dshi_3826 0.52 +0.0 33,478 - Dshi_3826 0.52 +0.9 33,515 + Dshi_3826 0.54 +0.1 33,666 + Dshi_3826 0.61 -0.6 33,994 - Dshi_3826 0.77 +0.9 34,047 + Dshi_3826 0.80 -0.8 34,196 - Dshi_3826 0.87 +1.8 34,212 + Dshi_3826 0.88 -0.3 34,212 + Dshi_3826 0.88 +0.7 34,354 + +0.1 34,491 + -0.1 34,535 - +0.3 34,656 + -0.0 34,712 + Dshi_3827 0.11 -0.1 34,756 - Dshi_3827 0.13 -1.3 34,817 + Dshi_3827 0.16 +0.3 34,817 + Dshi_3827 0.16 +0.2 34,825 - Dshi_3827 0.16 +1.0 34,825 - Dshi_3827 0.16 +0.7 34,929 - Dshi_3827 0.21 -0.8 34,986 - Dshi_3827 0.24 +0.1 35,169 + Dshi_3827 0.33 +0.1 35,169 + Dshi_3827 0.33 +0.1 35,306 + Dshi_3827 0.40 +0.5
Or see this region's nucleotide sequence