Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3720 and Dshi_3721 overlap by 13 nucleotides Dshi_3721 and Dshi_3722 overlap by 8 nucleotides Dshi_3722 and Dshi_3723 are separated by 26 nucleotides Dshi_3723 and Dshi_3724 are separated by 41 nucleotides
Dshi_3720: Dshi_3720 - molybdopterin dehydrogenase FAD-binding (RefSeq), at 127,602 to 128,429
_3720
Dshi_3721: Dshi_3721 - (2Fe-2S)-binding domain protein (RefSeq), at 128,417 to 128,926
_3721
Dshi_3722: Dshi_3722 - AMP-dependent synthetase and ligase (RefSeq), at 128,919 to 130,454
_3722
Dshi_3723: Dshi_3723 - Enoyl-CoA hydratase/isomerase (RefSeq), at 130,481 to 131,284
_3723
Dshi_3724: Dshi_3724 - Extracellular ligand-binding receptor (RefSeq), at 131,326 to 132,480
_3724
Position (kb)
128
129
130
131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 127.919 kb on - strand, within Dshi_3720 at 128.013 kb on - strand, within Dshi_3720 at 128.018 kb on - strand, within Dshi_3720 at 128.038 kb on + strand, within Dshi_3720 at 128.038 kb on + strand, within Dshi_3720 at 128.038 kb on + strand, within Dshi_3720 at 128.065 kb on + strand, within Dshi_3720 at 128.065 kb on + strand, within Dshi_3720 at 128.073 kb on - strand, within Dshi_3720 at 128.158 kb on - strand, within Dshi_3720 at 128.189 kb on + strand, within Dshi_3720 at 128.200 kb on + strand, within Dshi_3720 at 128.276 kb on + strand, within Dshi_3720 at 128.284 kb on - strand, within Dshi_3720 at 128.345 kb on + strand, within Dshi_3720 at 128.345 kb on + strand, within Dshi_3720 at 128.400 kb on - strand at 128.462 kb on - strand at 128.462 kb on - strand at 128.462 kb on - strand at 128.490 kb on - strand, within Dshi_3721 at 128.527 kb on + strand, within Dshi_3721 at 128.663 kb on + strand, within Dshi_3721 at 128.669 kb on + strand, within Dshi_3721 at 128.669 kb on + strand, within Dshi_3721 at 128.671 kb on - strand, within Dshi_3721 at 128.782 kb on + strand, within Dshi_3721 at 128.891 kb on + strand at 128.906 kb on - strand at 128.906 kb on - strand at 128.909 kb on + strand at 128.997 kb on + strand at 129.047 kb on - strand at 129.060 kb on + strand at 129.090 kb on + strand, within Dshi_3722 at 129.123 kb on + strand, within Dshi_3722 at 129.130 kb on - strand, within Dshi_3722 at 129.206 kb on + strand, within Dshi_3722 at 129.214 kb on - strand, within Dshi_3722 at 129.238 kb on + strand, within Dshi_3722 at 129.246 kb on - strand, within Dshi_3722 at 129.281 kb on - strand, within Dshi_3722 at 129.281 kb on - strand, within Dshi_3722 at 129.327 kb on - strand, within Dshi_3722 at 129.358 kb on + strand, within Dshi_3722 at 129.366 kb on - strand, within Dshi_3722 at 129.373 kb on - strand, within Dshi_3722 at 129.385 kb on - strand, within Dshi_3722 at 129.419 kb on + strand, within Dshi_3722 at 129.419 kb on + strand, within Dshi_3722 at 129.427 kb on - strand, within Dshi_3722 at 129.427 kb on - strand, within Dshi_3722 at 129.427 kb on - strand, within Dshi_3722 at 129.427 kb on - strand, within Dshi_3722 at 129.455 kb on + strand, within Dshi_3722 at 129.455 kb on + strand, within Dshi_3722 at 129.455 kb on + strand, within Dshi_3722 at 129.463 kb on - strand, within Dshi_3722 at 129.486 kb on - strand, within Dshi_3722 at 129.535 kb on + strand, within Dshi_3722 at 129.565 kb on - strand, within Dshi_3722 at 129.583 kb on + strand, within Dshi_3722 at 129.616 kb on + strand, within Dshi_3722 at 129.631 kb on + strand, within Dshi_3722 at 129.657 kb on - strand, within Dshi_3722 at 129.673 kb on + strand, within Dshi_3722 at 129.673 kb on + strand, within Dshi_3722 at 129.677 kb on + strand, within Dshi_3722 at 129.681 kb on - strand, within Dshi_3722 at 129.681 kb on - strand, within Dshi_3722 at 129.690 kb on + strand, within Dshi_3722 at 129.698 kb on - strand, within Dshi_3722 at 129.729 kb on + strand, within Dshi_3722 at 129.737 kb on - strand, within Dshi_3722 at 129.767 kb on + strand, within Dshi_3722 at 129.776 kb on + strand, within Dshi_3722 at 129.780 kb on - strand, within Dshi_3722 at 129.830 kb on + strand, within Dshi_3722 at 129.831 kb on + strand, within Dshi_3722 at 129.839 kb on - strand, within Dshi_3722 at 129.915 kb on + strand, within Dshi_3722 at 129.915 kb on + strand, within Dshi_3722 at 129.919 kb on + strand, within Dshi_3722 at 129.966 kb on + strand, within Dshi_3722 at 130.008 kb on - strand, within Dshi_3722 at 130.016 kb on - strand, within Dshi_3722 at 130.021 kb on + strand, within Dshi_3722 at 130.089 kb on + strand, within Dshi_3722 at 130.111 kb on - strand, within Dshi_3722 at 130.130 kb on + strand, within Dshi_3722 at 130.197 kb on + strand, within Dshi_3722 at 130.213 kb on + strand, within Dshi_3722 at 130.220 kb on - strand, within Dshi_3722 at 130.221 kb on - strand, within Dshi_3722 at 130.223 kb on + strand, within Dshi_3722 at 130.235 kb on - strand, within Dshi_3722 at 130.235 kb on - strand, within Dshi_3722 at 130.275 kb on + strand, within Dshi_3722 at 130.276 kb on - strand, within Dshi_3722 at 130.283 kb on + strand, within Dshi_3722 at 130.285 kb on + strand, within Dshi_3722 at 130.285 kb on + strand, within Dshi_3722 at 130.293 kb on - strand, within Dshi_3722 at 130.293 kb on - strand, within Dshi_3722 at 130.365 kb on - strand at 130.423 kb on + strand at 130.425 kb on + strand at 130.425 kb on + strand at 130.437 kb on - strand at 130.437 kb on - strand at 130.439 kb on - strand at 130.449 kb on + strand at 130.449 kb on + strand at 130.457 kb on - strand at 130.457 kb on - strand at 130.457 kb on - strand at 130.460 kb on + strand at 130.460 kb on + strand at 130.460 kb on + strand at 130.460 kb on + strand at 130.468 kb on - strand at 130.471 kb on - strand at 130.489 kb on + strand at 130.521 kb on - strand at 130.606 kb on + strand, within Dshi_3723 at 131.047 kb on + strand, within Dshi_3723 at 131.316 kb on - strand at 131.316 kb on - strand at 131.339 kb on - strand at 131.358 kb on - strand at 131.367 kb on - strand at 131.371 kb on + strand at 131.371 kb on + strand at 131.376 kb on + strand at 131.379 kb on - strand at 131.420 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 127,919 - Dshi_3720 0.38 +0.7 128,013 - Dshi_3720 0.50 +0.3 128,018 - Dshi_3720 0.50 +0.1 128,038 + Dshi_3720 0.53 +0.3 128,038 + Dshi_3720 0.53 -0.3 128,038 + Dshi_3720 0.53 +0.2 128,065 + Dshi_3720 0.56 -0.1 128,065 + Dshi_3720 0.56 -2.3 128,073 - Dshi_3720 0.57 +0.6 128,158 - Dshi_3720 0.67 +0.1 128,189 + Dshi_3720 0.71 -1.0 128,200 + Dshi_3720 0.72 -0.7 128,276 + Dshi_3720 0.81 -0.0 128,284 - Dshi_3720 0.82 +1.2 128,345 + Dshi_3720 0.90 +1.9 128,345 + Dshi_3720 0.90 +0.2 128,400 - -0.5 128,462 - -0.3 128,462 - +0.3 128,462 - -2.8 128,490 - Dshi_3721 0.14 +1.1 128,527 + Dshi_3721 0.22 -0.0 128,663 + Dshi_3721 0.48 -0.7 128,669 + Dshi_3721 0.49 +1.3 128,669 + Dshi_3721 0.49 +1.6 128,671 - Dshi_3721 0.50 +0.3 128,782 + Dshi_3721 0.72 +0.8 128,891 + -1.1 128,906 - +0.7 128,906 - +0.5 128,909 + +0.9 128,997 + +0.6 129,047 - +1.3 129,060 + +0.2 129,090 + Dshi_3722 0.11 -0.3 129,123 + Dshi_3722 0.13 +1.1 129,130 - Dshi_3722 0.14 -0.3 129,206 + Dshi_3722 0.19 +0.2 129,214 - Dshi_3722 0.19 -0.4 129,238 + Dshi_3722 0.21 +1.1 129,246 - Dshi_3722 0.21 +0.3 129,281 - Dshi_3722 0.24 -0.1 129,281 - Dshi_3722 0.24 +0.3 129,327 - Dshi_3722 0.27 -1.4 129,358 + Dshi_3722 0.29 +0.3 129,366 - Dshi_3722 0.29 +0.2 129,373 - Dshi_3722 0.30 -0.1 129,385 - Dshi_3722 0.30 +0.6 129,419 + Dshi_3722 0.33 +0.5 129,419 + Dshi_3722 0.33 +0.1 129,427 - Dshi_3722 0.33 +0.4 129,427 - Dshi_3722 0.33 +0.4 129,427 - Dshi_3722 0.33 -1.0 129,427 - Dshi_3722 0.33 -0.3 129,455 + Dshi_3722 0.35 +0.0 129,455 + Dshi_3722 0.35 -0.5 129,455 + Dshi_3722 0.35 +0.4 129,463 - Dshi_3722 0.35 -0.6 129,486 - Dshi_3722 0.37 -0.3 129,535 + Dshi_3722 0.40 +0.7 129,565 - Dshi_3722 0.42 -0.7 129,583 + Dshi_3722 0.43 -2.1 129,616 + Dshi_3722 0.45 +0.3 129,631 + Dshi_3722 0.46 +1.2 129,657 - Dshi_3722 0.48 -0.1 129,673 + Dshi_3722 0.49 -1.2 129,673 + Dshi_3722 0.49 +0.2 129,677 + Dshi_3722 0.49 +0.4 129,681 - Dshi_3722 0.50 +1.0 129,681 - Dshi_3722 0.50 +0.0 129,690 + Dshi_3722 0.50 -0.3 129,698 - Dshi_3722 0.51 +0.6 129,729 + Dshi_3722 0.53 -0.7 129,737 - Dshi_3722 0.53 +0.5 129,767 + Dshi_3722 0.55 -0.5 129,776 + Dshi_3722 0.56 -0.4 129,780 - Dshi_3722 0.56 +0.1 129,830 + Dshi_3722 0.59 +0.5 129,831 + Dshi_3722 0.59 +0.3 129,839 - Dshi_3722 0.60 -0.4 129,915 + Dshi_3722 0.65 +1.9 129,915 + Dshi_3722 0.65 -0.7 129,919 + Dshi_3722 0.65 +1.2 129,966 + Dshi_3722 0.68 +0.9 130,008 - Dshi_3722 0.71 +1.0 130,016 - Dshi_3722 0.71 +0.3 130,021 + Dshi_3722 0.72 -0.6 130,089 + Dshi_3722 0.76 +2.0 130,111 - Dshi_3722 0.78 -0.8 130,130 + Dshi_3722 0.79 +0.5 130,197 + Dshi_3722 0.83 +0.5 130,213 + Dshi_3722 0.84 -0.1 130,220 - Dshi_3722 0.85 +0.2 130,221 - Dshi_3722 0.85 +0.2 130,223 + Dshi_3722 0.85 -1.3 130,235 - Dshi_3722 0.86 +0.6 130,235 - Dshi_3722 0.86 +0.2 130,275 + Dshi_3722 0.88 +0.1 130,276 - Dshi_3722 0.88 -0.8 130,283 + Dshi_3722 0.89 +1.5 130,285 + Dshi_3722 0.89 +0.2 130,285 + Dshi_3722 0.89 -0.2 130,293 - Dshi_3722 0.89 +0.4 130,293 - Dshi_3722 0.89 +0.3 130,365 - +1.8 130,423 + +1.1 130,425 + +0.2 130,425 + +0.1 130,437 - +0.7 130,437 - +0.5 130,439 - +0.1 130,449 + -0.7 130,449 + +0.1 130,457 - -0.1 130,457 - +1.6 130,457 - -0.0 130,460 + +0.3 130,460 + -0.1 130,460 + +0.3 130,460 + +0.9 130,468 - +0.1 130,471 - -0.1 130,489 + -0.0 130,521 - +1.1 130,606 + Dshi_3723 0.16 +1.0 131,047 + Dshi_3723 0.70 -0.1 131,316 - +0.2 131,316 - -0.0 131,339 - +1.3 131,358 - +0.6 131,367 - +1.3 131,371 + +0.4 131,371 + +0.5 131,376 + -0.5 131,379 - +0.9 131,420 + -0.5
Or see this region's nucleotide sequence