Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2669 and Dshi_2670 overlap by 61 nucleotides Dshi_2670 and Dshi_2671 are separated by 16 nucleotides Dshi_2671 and Dshi_2672 are separated by 174 nucleotides Dshi_2672 and Dshi_2673 are separated by 297 nucleotides
Dshi_2669: Dshi_2669 - Sel1 domain protein repeat-containing protein (RefSeq), at 2,825,706 to 2,826,251
_2669
Dshi_2670: Dshi_2670 - ABC transporter related (RefSeq), at 2,826,191 to 2,826,901
_2670
Dshi_2671: Dshi_2671 - putative ABC transporter periplasmic solute-binding protein (RefSeq), at 2,826,918 to 2,827,889
_2671
Dshi_2672: Dshi_2672 - cytochrome c class I (RefSeq), at 2,828,064 to 2,828,483
_2672
Dshi_2673: Dshi_2673 - Pyrrolo-quinoline quinone (RefSeq), at 2,828,781 to 2,830,547
_2673
Position (kb)
2826
2827
2828 Strain fitness (log2 ratio)
-1
0
1 at 2825.926 kb on + strand, within Dshi_2669 at 2826.044 kb on + strand, within Dshi_2669 at 2826.076 kb on + strand, within Dshi_2669 at 2826.110 kb on + strand, within Dshi_2669 at 2826.195 kb on + strand at 2826.267 kb on - strand, within Dshi_2670 at 2826.350 kb on - strand, within Dshi_2670 at 2826.403 kb on + strand, within Dshi_2670 at 2826.451 kb on + strand, within Dshi_2670 at 2826.652 kb on - strand, within Dshi_2670 at 2827.030 kb on - strand, within Dshi_2671 at 2827.079 kb on - strand, within Dshi_2671 at 2827.157 kb on + strand, within Dshi_2671 at 2827.167 kb on + strand, within Dshi_2671 at 2827.170 kb on - strand, within Dshi_2671 at 2827.241 kb on + strand, within Dshi_2671 at 2827.257 kb on - strand, within Dshi_2671 at 2827.356 kb on + strand, within Dshi_2671 at 2827.402 kb on + strand, within Dshi_2671 at 2827.407 kb on + strand, within Dshi_2671 at 2827.410 kb on - strand, within Dshi_2671 at 2827.412 kb on + strand, within Dshi_2671 at 2827.420 kb on - strand, within Dshi_2671 at 2827.487 kb on - strand, within Dshi_2671 at 2827.546 kb on - strand, within Dshi_2671 at 2827.551 kb on - strand, within Dshi_2671 at 2827.559 kb on + strand, within Dshi_2671 at 2827.577 kb on - strand, within Dshi_2671 at 2827.596 kb on + strand, within Dshi_2671 at 2827.683 kb on - strand, within Dshi_2671 at 2827.783 kb on + strand, within Dshi_2671 at 2827.867 kb on + strand at 2827.874 kb on + strand at 2827.895 kb on - strand at 2827.917 kb on + strand at 2827.935 kb on + strand at 2827.944 kb on - strand at 2827.968 kb on + strand at 2828.014 kb on - strand at 2828.031 kb on + strand at 2828.039 kb on - strand at 2828.088 kb on + strand at 2828.088 kb on + strand at 2828.096 kb on - strand at 2828.108 kb on + strand, within Dshi_2672 at 2828.174 kb on + strand, within Dshi_2672 at 2828.182 kb on - strand, within Dshi_2672 at 2828.326 kb on + strand, within Dshi_2672 at 2828.402 kb on + strand, within Dshi_2672 at 2828.405 kb on + strand, within Dshi_2672 at 2828.420 kb on - strand, within Dshi_2672 at 2828.519 kb on - strand at 2828.576 kb on + strand at 2828.581 kb on + strand at 2828.594 kb on + strand at 2828.631 kb on - strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.720 kb on + strand at 2828.728 kb on - strand at 2828.728 kb on - strand at 2828.729 kb on + strand at 2828.757 kb on + strand at 2828.757 kb on + strand at 2828.821 kb on + strand at 2828.853 kb on + strand at 2828.861 kb on - strand at 2828.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 2,825,926 + Dshi_2669 0.40 +1.2 2,826,044 + Dshi_2669 0.62 +0.9 2,826,076 + Dshi_2669 0.68 +1.4 2,826,110 + Dshi_2669 0.74 +0.3 2,826,195 + +0.0 2,826,267 - Dshi_2670 0.11 -0.3 2,826,350 - Dshi_2670 0.22 +0.2 2,826,403 + Dshi_2670 0.30 +0.3 2,826,451 + Dshi_2670 0.37 +1.1 2,826,652 - Dshi_2670 0.65 -1.1 2,827,030 - Dshi_2671 0.12 +0.5 2,827,079 - Dshi_2671 0.17 -0.5 2,827,157 + Dshi_2671 0.25 -1.4 2,827,167 + Dshi_2671 0.26 -0.1 2,827,170 - Dshi_2671 0.26 +0.6 2,827,241 + Dshi_2671 0.33 +0.6 2,827,257 - Dshi_2671 0.35 +1.1 2,827,356 + Dshi_2671 0.45 +0.8 2,827,402 + Dshi_2671 0.50 +0.5 2,827,407 + Dshi_2671 0.50 +1.6 2,827,410 - Dshi_2671 0.51 +0.4 2,827,412 + Dshi_2671 0.51 +0.4 2,827,420 - Dshi_2671 0.52 +0.9 2,827,487 - Dshi_2671 0.59 +0.2 2,827,546 - Dshi_2671 0.65 +1.0 2,827,551 - Dshi_2671 0.65 +0.5 2,827,559 + Dshi_2671 0.66 +1.1 2,827,577 - Dshi_2671 0.68 +1.4 2,827,596 + Dshi_2671 0.70 +0.8 2,827,683 - Dshi_2671 0.79 -0.3 2,827,783 + Dshi_2671 0.89 +0.3 2,827,867 + -0.0 2,827,874 + -0.1 2,827,895 - -1.1 2,827,917 + -0.5 2,827,935 + -0.1 2,827,944 - +1.3 2,827,968 + +0.4 2,828,014 - +0.3 2,828,031 + +1.3 2,828,039 - +0.3 2,828,088 + -0.0 2,828,088 + +1.1 2,828,096 - -0.7 2,828,108 + Dshi_2672 0.10 -1.0 2,828,174 + Dshi_2672 0.26 -0.5 2,828,182 - Dshi_2672 0.28 +0.9 2,828,326 + Dshi_2672 0.62 +0.0 2,828,402 + Dshi_2672 0.80 +0.1 2,828,405 + Dshi_2672 0.81 +1.1 2,828,420 - Dshi_2672 0.85 +0.5 2,828,519 - +0.3 2,828,576 + +0.6 2,828,581 + -0.7 2,828,594 + +0.1 2,828,631 - +0.8 2,828,720 + -0.2 2,828,720 + +0.5 2,828,720 + +0.5 2,828,720 + +0.9 2,828,728 - +1.4 2,828,728 - -0.3 2,828,729 + +0.0 2,828,757 + +0.5 2,828,757 + +0.3 2,828,821 + -0.2 2,828,853 + -0.6 2,828,861 - +0.9 2,828,867 - -0.2
Or see this region's nucleotide sequence