Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1557 and Dshi_1558 overlap by 34 nucleotides Dshi_1558 and Dshi_1559 are separated by 115 nucleotides Dshi_1559 and Dshi_1560 are separated by 137 nucleotides Dshi_1560 and Dshi_1561 are separated by 132 nucleotides
Dshi_1557: Dshi_1557 - DSBA oxidoreductase (RefSeq), at 1,619,422 to 1,620,090
_1557
Dshi_1558: Dshi_1558 - hypothetical protein (RefSeq), at 1,620,057 to 1,620,377
_1558
Dshi_1559: Dshi_1559 - drug resistance transporter, Bcr/CflA subfamily (RefSeq), at 1,620,493 to 1,621,701
_1559
Dshi_1560: Dshi_1560 - hypothetical protein (RefSeq), at 1,621,839 to 1,622,270
_1560
Dshi_1561: Dshi_1561 - transcription-repair coupling factor (RefSeq), at 1,622,403 to 1,625,885
_1561
Position (kb)
1620
1621
1622 Strain fitness (log2 ratio)
-1
0
1 at 1619.692 kb on + strand, within Dshi_1557 at 1619.752 kb on - strand, within Dshi_1557 at 1620.354 kb on + strand at 1620.374 kb on + strand at 1620.505 kb on + strand at 1620.651 kb on - strand, within Dshi_1559 at 1620.768 kb on + strand, within Dshi_1559 at 1620.843 kb on + strand, within Dshi_1559 at 1620.878 kb on - strand, within Dshi_1559 at 1620.878 kb on - strand, within Dshi_1559 at 1621.143 kb on - strand, within Dshi_1559 at 1621.638 kb on - strand at 1621.646 kb on - strand at 1621.824 kb on + strand at 1622.044 kb on + strand, within Dshi_1560 at 1622.106 kb on + strand, within Dshi_1560 at 1622.358 kb on + strand at 1622.467 kb on + strand at 1622.649 kb on - strand at 1622.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,619,692 + Dshi_1557 0.40 -0.4 1,619,752 - Dshi_1557 0.49 +1.1 1,620,354 + -0.1 1,620,374 + +0.2 1,620,505 + +0.3 1,620,651 - Dshi_1559 0.13 +0.1 1,620,768 + Dshi_1559 0.23 -1.3 1,620,843 + Dshi_1559 0.29 +0.2 1,620,878 - Dshi_1559 0.32 +0.0 1,620,878 - Dshi_1559 0.32 -0.1 1,621,143 - Dshi_1559 0.54 -0.4 1,621,638 - +0.1 1,621,646 - +0.6 1,621,824 + -0.3 1,622,044 + Dshi_1560 0.47 +0.4 1,622,106 + Dshi_1560 0.62 +0.7 1,622,358 + -0.2 1,622,467 + +0.8 1,622,649 - +0.2 1,622,670 - -0.1
Or see this region's nucleotide sequence