Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0838 and Dshi_0839 are separated by 73 nucleotides Dshi_0839 and Dshi_0840 are separated by 29 nucleotides Dshi_0840 and Dshi_0841 are separated by 445 nucleotides
Dshi_0838: Dshi_0838 - acyl-CoA dehydrogenase domain protein (RefSeq), at 848,409 to 849,557
_0838
Dshi_0839: Dshi_0839 - acyl-CoA dehydrogenase domain protein (RefSeq), at 849,631 to 851,409
_0839
Dshi_0840: Dshi_0840 - transcriptional regulator, MerR family (RefSeq), at 851,439 to 851,840
_0840
Dshi_0841: Dshi_0841 - hypothetical protein (RefSeq), at 852,286 to 853,314
_0841
Position (kb)
849
850
851
852 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 848.666 kb on - strand, within Dshi_0838 at 848.667 kb on + strand, within Dshi_0838 at 848.694 kb on + strand, within Dshi_0838 at 848.697 kb on - strand, within Dshi_0838 at 848.725 kb on - strand, within Dshi_0838 at 848.959 kb on - strand, within Dshi_0838 at 848.980 kb on + strand, within Dshi_0838 at 849.010 kb on + strand, within Dshi_0838 at 849.012 kb on + strand, within Dshi_0838 at 849.263 kb on - strand, within Dshi_0838 at 849.394 kb on + strand, within Dshi_0838 at 849.397 kb on - strand, within Dshi_0838 at 849.407 kb on - strand, within Dshi_0838 at 849.547 kb on + strand at 849.547 kb on - strand at 849.550 kb on + strand at 849.550 kb on - strand at 849.552 kb on + strand at 849.558 kb on - strand at 849.560 kb on - strand at 849.622 kb on + strand at 849.698 kb on - strand at 849.823 kb on - strand, within Dshi_0839 at 849.825 kb on + strand, within Dshi_0839 at 849.828 kb on - strand, within Dshi_0839 at 849.828 kb on - strand, within Dshi_0839 at 849.833 kb on - strand, within Dshi_0839 at 849.926 kb on + strand, within Dshi_0839 at 849.996 kb on - strand, within Dshi_0839 at 850.162 kb on + strand, within Dshi_0839 at 850.207 kb on + strand, within Dshi_0839 at 850.316 kb on - strand, within Dshi_0839 at 850.558 kb on + strand, within Dshi_0839 at 850.766 kb on + strand, within Dshi_0839 at 850.779 kb on - strand, within Dshi_0839 at 850.790 kb on - strand, within Dshi_0839 at 850.803 kb on + strand, within Dshi_0839 at 850.869 kb on + strand, within Dshi_0839 at 850.877 kb on - strand, within Dshi_0839 at 850.882 kb on - strand, within Dshi_0839 at 850.893 kb on - strand, within Dshi_0839 at 850.914 kb on - strand, within Dshi_0839 at 850.973 kb on + strand, within Dshi_0839 at 851.076 kb on - strand, within Dshi_0839 at 851.134 kb on - strand, within Dshi_0839 at 851.184 kb on + strand, within Dshi_0839 at 851.192 kb on - strand, within Dshi_0839 at 851.250 kb on - strand at 851.250 kb on - strand at 851.302 kb on - strand at 851.396 kb on + strand at 851.495 kb on - strand, within Dshi_0840 at 851.645 kb on + strand, within Dshi_0840 at 851.691 kb on - strand, within Dshi_0840 at 851.692 kb on - strand, within Dshi_0840 at 851.712 kb on - strand, within Dshi_0840 at 851.752 kb on + strand, within Dshi_0840 at 851.760 kb on - strand, within Dshi_0840 at 851.783 kb on + strand, within Dshi_0840 at 851.783 kb on + strand, within Dshi_0840 at 851.783 kb on + strand, within Dshi_0840 at 851.793 kb on - strand, within Dshi_0840 at 851.831 kb on - strand at 851.833 kb on - strand at 851.865 kb on - strand at 851.958 kb on + strand at 851.992 kb on + strand at 852.012 kb on + strand at 852.012 kb on + strand at 852.058 kb on - strand at 852.059 kb on + strand at 852.081 kb on - strand at 852.113 kb on - strand at 852.114 kb on - strand at 852.118 kb on + strand at 852.118 kb on + strand at 852.118 kb on + strand at 852.118 kb on + strand at 852.126 kb on - strand at 852.126 kb on - strand at 852.141 kb on + strand at 852.189 kb on - strand at 852.236 kb on + strand at 852.249 kb on - strand at 852.257 kb on - strand at 852.309 kb on + strand at 852.317 kb on + strand at 852.317 kb on - strand at 852.317 kb on - strand at 852.317 kb on - strand at 852.317 kb on - strand at 852.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 848,666 - Dshi_0838 0.22 -0.4 848,667 + Dshi_0838 0.22 +1.3 848,694 + Dshi_0838 0.25 +0.9 848,697 - Dshi_0838 0.25 +0.7 848,725 - Dshi_0838 0.28 -0.2 848,959 - Dshi_0838 0.48 +0.4 848,980 + Dshi_0838 0.50 +0.7 849,010 + Dshi_0838 0.52 +0.2 849,012 + Dshi_0838 0.52 -1.4 849,263 - Dshi_0838 0.74 -1.1 849,394 + Dshi_0838 0.86 +0.9 849,397 - Dshi_0838 0.86 -0.3 849,407 - Dshi_0838 0.87 +0.7 849,547 + -0.5 849,547 - -0.7 849,550 + +0.8 849,550 - +0.3 849,552 + +1.4 849,558 - +1.5 849,560 - +0.4 849,622 + +0.5 849,698 - +0.1 849,823 - Dshi_0839 0.11 +0.1 849,825 + Dshi_0839 0.11 -1.1 849,828 - Dshi_0839 0.11 +0.4 849,828 - Dshi_0839 0.11 +0.7 849,833 - Dshi_0839 0.11 +0.1 849,926 + Dshi_0839 0.17 +0.7 849,996 - Dshi_0839 0.21 +0.3 850,162 + Dshi_0839 0.30 +0.3 850,207 + Dshi_0839 0.32 -2.6 850,316 - Dshi_0839 0.39 +0.2 850,558 + Dshi_0839 0.52 +1.4 850,766 + Dshi_0839 0.64 +0.8 850,779 - Dshi_0839 0.65 +1.3 850,790 - Dshi_0839 0.65 +0.5 850,803 + Dshi_0839 0.66 -2.8 850,869 + Dshi_0839 0.70 -0.1 850,877 - Dshi_0839 0.70 +0.5 850,882 - Dshi_0839 0.70 +2.4 850,893 - Dshi_0839 0.71 +0.3 850,914 - Dshi_0839 0.72 -0.1 850,973 + Dshi_0839 0.75 +0.7 851,076 - Dshi_0839 0.81 +0.7 851,134 - Dshi_0839 0.84 +0.4 851,184 + Dshi_0839 0.87 -1.1 851,192 - Dshi_0839 0.88 -0.0 851,250 - -0.6 851,250 - -0.5 851,302 - +0.1 851,396 + +0.1 851,495 - Dshi_0840 0.14 -0.1 851,645 + Dshi_0840 0.51 -0.1 851,691 - Dshi_0840 0.63 -0.6 851,692 - Dshi_0840 0.63 -0.1 851,712 - Dshi_0840 0.68 -0.6 851,752 + Dshi_0840 0.78 +1.2 851,760 - Dshi_0840 0.80 -0.5 851,783 + Dshi_0840 0.86 -0.6 851,783 + Dshi_0840 0.86 +1.9 851,783 + Dshi_0840 0.86 +0.3 851,793 - Dshi_0840 0.88 +1.0 851,831 - +0.2 851,833 - -0.1 851,865 - -0.5 851,958 + -0.2 851,992 + +0.4 852,012 + +0.9 852,012 + -2.2 852,058 - +0.0 852,059 + -0.1 852,081 - +0.0 852,113 - +0.9 852,114 - -0.8 852,118 + -1.0 852,118 + +1.2 852,118 + +0.9 852,118 + -1.3 852,126 - +0.3 852,126 - -1.1 852,141 + +0.7 852,189 - +0.9 852,236 + +1.3 852,249 - +0.5 852,257 - +0.3 852,309 + -1.3 852,317 + +0.2 852,317 - +0.8 852,317 - +0.5 852,317 - +1.3 852,317 - +0.4 852,376 - +0.3
Or see this region's nucleotide sequence