Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0512 and Dshi_0513 are separated by 736 nucleotides Dshi_0513 and Dshi_0514 overlap by 22 nucleotides Dshi_0514 and Dshi_0515 overlap by 4 nucleotides
Dshi_0512: Dshi_0512 - beta-lactamase domain protein (RefSeq), at 496,905 to 497,582
_0512
Dshi_0513: Dshi_0513 - transcriptional regulator, LuxR family (RefSeq), at 498,319 to 498,786
_0513
Dshi_0514: Dshi_0514 - response regulator receiver protein (RefSeq), at 498,765 to 498,947
_0514
Dshi_0515: Dshi_0515 - histidine kinase (RefSeq), at 498,944 to 499,843
_0515
Position (kb)
498
499 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 497.326 kb on + strand, within Dshi_0512 at 497.378 kb on + strand, within Dshi_0512 at 497.489 kb on + strand, within Dshi_0512 at 497.497 kb on - strand, within Dshi_0512 at 497.497 kb on - strand, within Dshi_0512 at 497.529 kb on + strand at 497.545 kb on - strand at 497.711 kb on - strand at 497.743 kb on - strand at 497.796 kb on + strand at 497.805 kb on + strand at 497.805 kb on + strand at 497.874 kb on - strand at 497.907 kb on - strand at 497.907 kb on - strand at 498.032 kb on + strand at 498.040 kb on - strand at 498.040 kb on - strand at 498.046 kb on + strand at 498.046 kb on + strand at 498.073 kb on - strand at 498.172 kb on + strand at 498.368 kb on + strand, within Dshi_0513 at 498.431 kb on + strand, within Dshi_0513 at 498.485 kb on - strand, within Dshi_0513 at 498.504 kb on + strand, within Dshi_0513 at 498.504 kb on + strand, within Dshi_0513 at 498.512 kb on - strand, within Dshi_0513 at 498.512 kb on - strand, within Dshi_0513 at 498.512 kb on - strand, within Dshi_0513 at 498.539 kb on + strand, within Dshi_0513 at 498.539 kb on + strand, within Dshi_0513 at 498.574 kb on + strand, within Dshi_0513 at 498.595 kb on + strand, within Dshi_0513 at 498.708 kb on + strand, within Dshi_0513 at 498.740 kb on - strand at 498.776 kb on - strand at 498.785 kb on + strand at 498.801 kb on + strand, within Dshi_0514 at 498.831 kb on + strand, within Dshi_0514 at 498.908 kb on - strand, within Dshi_0514 at 498.925 kb on + strand, within Dshi_0514 at 499.074 kb on - strand, within Dshi_0515 at 499.107 kb on + strand, within Dshi_0515 at 499.115 kb on - strand, within Dshi_0515 at 499.117 kb on + strand, within Dshi_0515 at 499.174 kb on + strand, within Dshi_0515 at 499.182 kb on - strand, within Dshi_0515 at 499.244 kb on + strand, within Dshi_0515 at 499.293 kb on + strand, within Dshi_0515 at 499.293 kb on + strand, within Dshi_0515 at 499.296 kb on - strand, within Dshi_0515 at 499.359 kb on - strand, within Dshi_0515 at 499.389 kb on + strand, within Dshi_0515 at 499.397 kb on - strand, within Dshi_0515 at 499.433 kb on + strand, within Dshi_0515 at 499.459 kb on - strand, within Dshi_0515 at 499.501 kb on + strand, within Dshi_0515 at 499.501 kb on + strand, within Dshi_0515 at 499.570 kb on + strand, within Dshi_0515 at 499.617 kb on - strand, within Dshi_0515 at 499.643 kb on - strand, within Dshi_0515 at 499.654 kb on - strand, within Dshi_0515 at 499.664 kb on - strand, within Dshi_0515 at 499.700 kb on + strand, within Dshi_0515 at 499.768 kb on - strand at 499.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 497,326 + Dshi_0512 0.62 +0.5 497,378 + Dshi_0512 0.70 +0.1 497,489 + Dshi_0512 0.86 -0.6 497,497 - Dshi_0512 0.87 +0.9 497,497 - Dshi_0512 0.87 +0.2 497,529 + -0.4 497,545 - +0.1 497,711 - +0.1 497,743 - -2.3 497,796 + +0.6 497,805 + -2.8 497,805 + +0.1 497,874 - +0.1 497,907 - -1.0 497,907 - +0.5 498,032 + -1.3 498,040 - +0.8 498,040 - +0.5 498,046 + +0.3 498,046 + +0.7 498,073 - +0.7 498,172 + +0.1 498,368 + Dshi_0513 0.10 +0.2 498,431 + Dshi_0513 0.24 -0.8 498,485 - Dshi_0513 0.35 -0.1 498,504 + Dshi_0513 0.40 -0.9 498,504 + Dshi_0513 0.40 +0.6 498,512 - Dshi_0513 0.41 -0.5 498,512 - Dshi_0513 0.41 -0.1 498,512 - Dshi_0513 0.41 -0.0 498,539 + Dshi_0513 0.47 -0.9 498,539 + Dshi_0513 0.47 -0.6 498,574 + Dshi_0513 0.54 -0.7 498,595 + Dshi_0513 0.59 -0.2 498,708 + Dshi_0513 0.83 +0.1 498,740 - -0.9 498,776 - +1.1 498,785 + -0.6 498,801 + Dshi_0514 0.20 +0.1 498,831 + Dshi_0514 0.36 +0.4 498,908 - Dshi_0514 0.78 -0.4 498,925 + Dshi_0514 0.87 -0.8 499,074 - Dshi_0515 0.14 -0.3 499,107 + Dshi_0515 0.18 -0.5 499,115 - Dshi_0515 0.19 +0.2 499,117 + Dshi_0515 0.19 +0.6 499,174 + Dshi_0515 0.26 -0.7 499,182 - Dshi_0515 0.26 -0.3 499,244 + Dshi_0515 0.33 -1.7 499,293 + Dshi_0515 0.39 +1.6 499,293 + Dshi_0515 0.39 -0.5 499,296 - Dshi_0515 0.39 +0.2 499,359 - Dshi_0515 0.46 +0.4 499,389 + Dshi_0515 0.49 -0.8 499,397 - Dshi_0515 0.50 -0.4 499,433 + Dshi_0515 0.54 +0.2 499,459 - Dshi_0515 0.57 -0.2 499,501 + Dshi_0515 0.62 -0.2 499,501 + Dshi_0515 0.62 +0.7 499,570 + Dshi_0515 0.70 -0.6 499,617 - Dshi_0515 0.75 +1.0 499,643 - Dshi_0515 0.78 +0.2 499,654 - Dshi_0515 0.79 +0.7 499,664 - Dshi_0515 0.80 -1.1 499,700 + Dshi_0515 0.84 +0.5 499,768 - -0.1 499,781 - -0.9
Or see this region's nucleotide sequence