Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_760 and HP15_761 are separated by 37 nucleotides HP15_761 and HP15_762 are separated by 114 nucleotides HP15_762 and HP15_763 are separated by 59 nucleotides
HP15_760: HP15_760 - tRNA (guanine-N1)-methyltransferase, at 769,858 to 770,604
_760
HP15_761: HP15_761 - ribosomal protein L19, at 770,642 to 770,998
_761
HP15_762: HP15_762 - site-specific tyrosine recombinase XerD, at 771,113 to 772,015
_762
HP15_763: HP15_763 - protein-disulfide isomerase, at 772,075 to 772,875
_763
Position (kb) 771
772
773 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 771.106 kb on - strand at 771.628 kb on - strand, within HP15_762 at 771.748 kb on + strand, within HP15_762 at 772.273 kb on - strand, within HP15_763 at 772.352 kb on + strand, within HP15_763 at 772.396 kb on - strand, within HP15_763 at 772.414 kb on - strand, within HP15_763 at 772.706 kb on - strand, within HP15_763 at 772.763 kb on - strand, within HP15_763 at 772.840 kb on + strand at 772.842 kb on - strand at 772.859 kb on + strand at 772.939 kb on - strand at 772.941 kb on + strand at 772.951 kb on + strand at 772.959 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 771,106 - +0.2 771,628 - HP15_762 0.57 -3.6 771,748 + HP15_762 0.70 -1.2 772,273 - HP15_763 0.25 +0.0 772,352 + HP15_763 0.35 -0.2 772,396 - HP15_763 0.40 +1.2 772,414 - HP15_763 0.42 -1.7 772,706 - HP15_763 0.79 -0.4 772,763 - HP15_763 0.86 +0.1 772,840 + +0.1 772,842 - +0.6 772,859 + -0.1 772,939 - -0.1 772,941 + +0.2 772,951 + -0.7 772,959 - -0.1
Or see this region's nucleotide sequence