Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_716 and HP15_717 are separated by 51 nucleotides HP15_717 and HP15_718 are separated by 40 nucleotides HP15_718 and HP15_719 overlap by 4 nucleotides HP15_719 and HP15_720 are separated by 3 nucleotides
HP15_716: HP15_716 - conserved hypothetical protein, at 725,509 to 726,318
_716
HP15_717: HP15_717 - MscS Mechanosensitive ion channel, at 726,370 to 727,230
_717
HP15_718: HP15_718 - ribosomal protein S6 modification protein-like protein, at 727,271 to 727,708
_718
HP15_719: HP15_719 - alpha-L-glutamate ligase, RimK family, at 727,705 to 728,610
_719
HP15_720: HP15_720 - succinylglutamate desuccinylase/aspartoacylase family protein, at 728,614 to 729,678
_720
Position (kb)
727
728 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 726.499 kb on - strand, within HP15_717 at 726.648 kb on - strand, within HP15_717 at 726.683 kb on - strand, within HP15_717 at 726.727 kb on - strand, within HP15_717 at 726.757 kb on - strand, within HP15_717 at 726.762 kb on - strand, within HP15_717 at 726.809 kb on + strand, within HP15_717 at 726.824 kb on + strand, within HP15_717 at 726.832 kb on - strand, within HP15_717 at 726.832 kb on - strand, within HP15_717 at 726.871 kb on - strand, within HP15_717 at 726.875 kb on - strand, within HP15_717 at 726.891 kb on + strand, within HP15_717 at 726.909 kb on + strand, within HP15_717 at 726.971 kb on - strand, within HP15_717 at 727.156 kb on - strand at 727.166 kb on - strand at 727.293 kb on + strand at 727.372 kb on - strand, within HP15_718 at 727.377 kb on + strand, within HP15_718 at 727.377 kb on + strand, within HP15_718 at 727.425 kb on - strand, within HP15_718 at 727.460 kb on - strand, within HP15_718 at 727.665 kb on + strand at 727.688 kb on + strand at 727.737 kb on + strand at 727.948 kb on - strand, within HP15_719 at 728.035 kb on + strand, within HP15_719 at 728.035 kb on + strand, within HP15_719 at 728.179 kb on + strand, within HP15_719 at 728.318 kb on - strand, within HP15_719 at 728.326 kb on + strand, within HP15_719 at 728.349 kb on - strand, within HP15_719 at 728.425 kb on + strand, within HP15_719 at 728.426 kb on + strand, within HP15_719 at 728.433 kb on - strand, within HP15_719 at 728.441 kb on + strand, within HP15_719 at 728.449 kb on - strand, within HP15_719 at 728.577 kb on + strand at 728.613 kb on - strand at 728.649 kb on + strand at 728.694 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 726,499 - HP15_717 0.15 -0.2 726,648 - HP15_717 0.32 -0.1 726,683 - HP15_717 0.36 -0.8 726,727 - HP15_717 0.41 +0.1 726,757 - HP15_717 0.45 -0.6 726,762 - HP15_717 0.46 +0.2 726,809 + HP15_717 0.51 +1.1 726,824 + HP15_717 0.53 +0.2 726,832 - HP15_717 0.54 -0.4 726,832 - HP15_717 0.54 +0.2 726,871 - HP15_717 0.58 -0.4 726,875 - HP15_717 0.59 -0.3 726,891 + HP15_717 0.61 -0.3 726,909 + HP15_717 0.63 +0.1 726,971 - HP15_717 0.70 +0.1 727,156 - -0.5 727,166 - +0.8 727,293 + -3.8 727,372 - HP15_718 0.23 -1.1 727,377 + HP15_718 0.24 -2.0 727,377 + HP15_718 0.24 -3.0 727,425 - HP15_718 0.35 -1.4 727,460 - HP15_718 0.43 -1.1 727,665 + -4.2 727,688 + -0.0 727,737 + +0.3 727,948 - HP15_719 0.27 -2.7 728,035 + HP15_719 0.36 +1.2 728,035 + HP15_719 0.36 -1.5 728,179 + HP15_719 0.52 -0.8 728,318 - HP15_719 0.68 -0.7 728,326 + HP15_719 0.69 -1.7 728,349 - HP15_719 0.71 +0.3 728,425 + HP15_719 0.79 +0.2 728,426 + HP15_719 0.80 -1.8 728,433 - HP15_719 0.80 -0.8 728,441 + HP15_719 0.81 +0.0 728,449 - HP15_719 0.82 -1.0 728,577 + +0.4 728,613 - -0.3 728,649 + -0.7 728,694 + -0.0
Or see this region's nucleotide sequence