Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_703 and HP15_tRNA14 are separated by 21 nucleotides HP15_tRNA14 and HP15_704 are separated by 92 nucleotides HP15_704 and HP15_705 are separated by 37 nucleotides HP15_705 and HP15_706 are separated by 30 nucleotides
HP15_703: HP15_703 - phage integrase, at 711,588 to 712,454
_703
HP15_tRNA14: HP15_tRNA14 - tRNA-Met, at 712,476 to 712,552
_tRNA14
HP15_704: HP15_704 - translation-associated GTPase, at 712,645 to 713,736
_704
HP15_705: HP15_705 - peptidyl-tRNA hydrolase, at 713,774 to 714,361
_705
HP15_706: HP15_706 - ribosomal 5S rRNA E-loop binding protein Ctc/L25/TL5, at 714,392 to 715,063
_706
Position (kb) 712
713
714 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 711.854 kb on - strand, within HP15_703 at 711.856 kb on + strand, within HP15_703 at 711.861 kb on + strand, within HP15_703 at 711.861 kb on + strand, within HP15_703 at 711.874 kb on - strand, within HP15_703 at 711.904 kb on - strand, within HP15_703 at 712.064 kb on + strand, within HP15_703 at 712.177 kb on + strand, within HP15_703 at 712.180 kb on - strand, within HP15_703 at 712.180 kb on - strand, within HP15_703 at 712.240 kb on + strand, within HP15_703 at 712.300 kb on + strand, within HP15_703 at 712.334 kb on + strand, within HP15_703 at 712.342 kb on - strand, within HP15_703 at 712.617 kb on + strand at 713.172 kb on - strand, within HP15_704 at 713.366 kb on - strand, within HP15_704 at 713.384 kb on - strand, within HP15_704 at 713.384 kb on - strand, within HP15_704 at 713.412 kb on + strand, within HP15_704 at 713.675 kb on + strand at 713.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 711,854 - HP15_703 0.31 +0.2 711,856 + HP15_703 0.31 +0.4 711,861 + HP15_703 0.31 +0.2 711,861 + HP15_703 0.31 +0.1 711,874 - HP15_703 0.33 +0.1 711,904 - HP15_703 0.36 -0.7 712,064 + HP15_703 0.55 +0.4 712,177 + HP15_703 0.68 -0.3 712,180 - HP15_703 0.68 -0.5 712,180 - HP15_703 0.68 -0.2 712,240 + HP15_703 0.75 +0.5 712,300 + HP15_703 0.82 -0.2 712,334 + HP15_703 0.86 +2.4 712,342 - HP15_703 0.87 -0.5 712,617 + +0.2 713,172 - HP15_704 0.48 -1.3 713,366 - HP15_704 0.66 -0.8 713,384 - HP15_704 0.68 -1.0 713,384 - HP15_704 0.68 -3.0 713,412 + HP15_704 0.70 +0.4 713,675 + +0.5 713,772 + +0.6
Or see this region's nucleotide sequence