Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_351 and HP15_352 are separated by 25 nucleotides HP15_352 and HP15_353 are separated by 16 nucleotides HP15_353 and HP15_354 are separated by 1 nucleotides
HP15_351: HP15_351 - Cl-channel, voltage-gated family protein, at 345,224 to 346,999
_351
HP15_352: HP15_352 - N-acetyl-gamma-glutamyl-phosphate reductase, at 347,025 to 348,191
_352
HP15_353: HP15_353 - conserved hypothetical protein, at 348,208 to 348,948
_353
HP15_354: HP15_354 - protein containing DUF583, at 348,950 to 349,435
_354
Position (kb) 347
348
349 Strain fitness (log2 ratio) -2
-1
0
1 at 346.030 kb on + strand, within HP15_351 at 346.105 kb on - strand, within HP15_351 at 346.214 kb on - strand, within HP15_351 at 346.219 kb on + strand, within HP15_351 at 346.375 kb on - strand, within HP15_351 at 346.531 kb on + strand, within HP15_351 at 346.738 kb on + strand, within HP15_351 at 347.110 kb on + strand at 347.204 kb on + strand, within HP15_352 at 347.391 kb on + strand, within HP15_352 at 347.507 kb on - strand, within HP15_352 at 347.924 kb on + strand, within HP15_352 at 347.992 kb on + strand, within HP15_352 at 348.046 kb on + strand, within HP15_352 at 348.423 kb on + strand, within HP15_353 at 348.838 kb on - strand, within HP15_353 at 348.917 kb on + strand at 348.925 kb on - strand at 349.112 kb on + strand, within HP15_354
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 346,030 + HP15_351 0.45 -0.8 346,105 - HP15_351 0.50 -0.3 346,214 - HP15_351 0.56 -0.0 346,219 + HP15_351 0.56 -1.3 346,375 - HP15_351 0.65 -0.7 346,531 + HP15_351 0.74 +1.5 346,738 + HP15_351 0.85 -0.6 347,110 + +0.2 347,204 + HP15_352 0.15 -2.2 347,391 + HP15_352 0.31 -1.3 347,507 - HP15_352 0.41 -1.8 347,924 + HP15_352 0.77 -2.2 347,992 + HP15_352 0.83 -1.2 348,046 + HP15_352 0.87 -1.8 348,423 + HP15_353 0.29 +0.5 348,838 - HP15_353 0.85 +0.7 348,917 + -0.6 348,925 - +0.8 349,112 + HP15_354 0.33 -0.2
Or see this region's nucleotide sequence