Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_319 and HP15_320 are separated by 95 nucleotides HP15_320 and HP15_321 overlap by 4 nucleotides HP15_321 and HP15_322 overlap by 18 nucleotides
HP15_319: HP15_319 - predicted membrane protein, at 316,724 to 317,314
_319
HP15_320: HP15_320 - dihydroneopterin aldolase, at 317,410 to 317,811
_320
HP15_321: HP15_321 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 317,808 to 318,335
_321
HP15_322: HP15_322 - glucose-6-phosphate isomerase, at 318,318 to 319,979
_322
Position (kb)
317
318
319 Strain fitness (log2 ratio)
-1
0
1 at 316.818 kb on - strand, within HP15_319 at 316.959 kb on - strand, within HP15_319 at 316.962 kb on + strand, within HP15_319 at 317.041 kb on + strand, within HP15_319 at 317.122 kb on + strand, within HP15_319 at 317.194 kb on - strand, within HP15_319 at 317.248 kb on + strand, within HP15_319 at 317.390 kb on + strand at 317.803 kb on + strand at 317.878 kb on - strand, within HP15_321 at 317.928 kb on + strand, within HP15_321 at 318.116 kb on - strand, within HP15_321 at 318.278 kb on + strand, within HP15_321 at 318.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 316,818 - HP15_319 0.16 -0.5 316,959 - HP15_319 0.40 +0.4 316,962 + HP15_319 0.40 -0.3 317,041 + HP15_319 0.54 +1.0 317,122 + HP15_319 0.67 +0.3 317,194 - HP15_319 0.80 -0.1 317,248 + HP15_319 0.89 +0.2 317,390 + -0.4 317,803 + -0.6 317,878 - HP15_321 0.13 -0.6 317,928 + HP15_321 0.23 -0.7 318,116 - HP15_321 0.58 +0.6 318,278 + HP15_321 0.89 -0.6 318,291 + -0.7
Or see this region's nucleotide sequence