Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_305 and HP15_306 are separated by 78 nucleotides HP15_306 and HP15_307 are separated by 61 nucleotides
HP15_305: HP15_305 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 294,961 to 297,102
_305
HP15_306: HP15_306 - YjgF-like protein, at 297,181 to 297,567
_306
HP15_307: HP15_307 - NAD-dependent epimerase/dehydratase, at 297,629 to 298,495
_307
Position (kb) 297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 296.603 kb on + strand, within HP15_305 at 296.603 kb on + strand, within HP15_305 at 296.603 kb on + strand, within HP15_305 at 296.797 kb on - strand, within HP15_305 at 296.877 kb on + strand, within HP15_305 at 296.877 kb on + strand, within HP15_305 at 297.010 kb on + strand at 297.044 kb on - strand at 297.265 kb on - strand, within HP15_306 at 297.357 kb on + strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.365 kb on - strand, within HP15_306 at 297.430 kb on - strand, within HP15_306 at 297.488 kb on - strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.519 kb on + strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.527 kb on - strand, within HP15_306 at 297.627 kb on + strand at 297.698 kb on + strand at 297.805 kb on + strand, within HP15_307 at 297.813 kb on - strand, within HP15_307 at 297.845 kb on + strand, within HP15_307 at 297.850 kb on + strand, within HP15_307 at 297.906 kb on - strand, within HP15_307 at 297.908 kb on + strand, within HP15_307 at 297.934 kb on + strand, within HP15_307 at 297.964 kb on + strand, within HP15_307 at 298.080 kb on + strand, within HP15_307 at 298.221 kb on + strand, within HP15_307 at 298.231 kb on - strand, within HP15_307 at 298.241 kb on + strand, within HP15_307 at 298.256 kb on + strand, within HP15_307 at 298.304 kb on - strand, within HP15_307 at 298.333 kb on + strand, within HP15_307 at 298.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 296,603 + HP15_305 0.77 -0.9 296,603 + HP15_305 0.77 +0.1 296,603 + HP15_305 0.77 +0.0 296,797 - HP15_305 0.86 -0.3 296,877 + HP15_305 0.89 -0.4 296,877 + HP15_305 0.89 -0.1 297,010 + -2.1 297,044 - -0.5 297,265 - HP15_306 0.22 -2.5 297,357 + HP15_306 0.45 -1.0 297,365 - HP15_306 0.48 -1.2 297,365 - HP15_306 0.48 -0.9 297,365 - HP15_306 0.48 -1.3 297,365 - HP15_306 0.48 +1.7 297,365 - HP15_306 0.48 -2.1 297,365 - HP15_306 0.48 -1.4 297,365 - HP15_306 0.48 -1.9 297,430 - HP15_306 0.64 +1.3 297,488 - HP15_306 0.79 -1.3 297,519 + HP15_306 0.87 -1.3 297,519 + HP15_306 0.87 -1.7 297,519 + HP15_306 0.87 -3.1 297,519 + HP15_306 0.87 -1.2 297,519 + HP15_306 0.87 -1.4 297,527 - HP15_306 0.89 -0.9 297,527 - HP15_306 0.89 -0.6 297,527 - HP15_306 0.89 -1.4 297,527 - HP15_306 0.89 -1.7 297,527 - HP15_306 0.89 -2.6 297,627 + +1.2 297,698 + +2.3 297,805 + HP15_307 0.20 -0.0 297,813 - HP15_307 0.21 -0.9 297,845 + HP15_307 0.25 +0.0 297,850 + HP15_307 0.25 +0.1 297,906 - HP15_307 0.32 +0.0 297,908 + HP15_307 0.32 +1.0 297,934 + HP15_307 0.35 -0.5 297,964 + HP15_307 0.39 +0.7 298,080 + HP15_307 0.52 +0.5 298,221 + HP15_307 0.68 -0.2 298,231 - HP15_307 0.69 -0.2 298,241 + HP15_307 0.71 -1.6 298,256 + HP15_307 0.72 -0.6 298,304 - HP15_307 0.78 -0.0 298,333 + HP15_307 0.81 +0.2 298,479 - +0.2
Or see this region's nucleotide sequence