Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24095 and BWI76_RS24100 are separated by 16 nucleotides BWI76_RS24100 and BWI76_RS24105 are separated by 8 nucleotides BWI76_RS24105 and BWI76_RS24110 overlap by 4 nucleotides BWI76_RS24110 and BWI76_RS24115 are separated by 12 nucleotides
BWI76_RS24095: BWI76_RS24095 - twitching motility protein PilT, at 5,026,489 to 5,027,469
_RS24095
BWI76_RS24100: BWI76_RS24100 - YggS family pyridoxal phosphate enzyme, at 5,027,486 to 5,028,199
_RS24100
BWI76_RS24105: BWI76_RS24105 - resistance protein, at 5,028,208 to 5,028,774
_RS24105
BWI76_RS24110: BWI76_RS24110 - YggU family protein, at 5,028,771 to 5,029,061
_RS24110
BWI76_RS24115: BWI76_RS24115 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 5,029,074 to 5,029,667
_RS24115
Position (kb)
5027
5028
5029 Strain fitness (log2 ratio)
-2
-1
0
1 at 5026.490 kb on + strand at 5026.490 kb on + strand at 5026.495 kb on - strand at 5026.495 kb on - strand at 5026.496 kb on - strand at 5026.498 kb on + strand at 5026.512 kb on - strand at 5026.513 kb on - strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.563 kb on + strand at 5026.571 kb on - strand at 5026.586 kb on + strand at 5026.594 kb on - strand, within BWI76_RS24095 at 5026.612 kb on - strand, within BWI76_RS24095 at 5026.622 kb on - strand, within BWI76_RS24095 at 5026.624 kb on - strand, within BWI76_RS24095 at 5026.637 kb on - strand, within BWI76_RS24095 at 5026.647 kb on - strand, within BWI76_RS24095 at 5026.657 kb on - strand, within BWI76_RS24095 at 5026.667 kb on + strand, within BWI76_RS24095 at 5026.776 kb on + strand, within BWI76_RS24095 at 5026.784 kb on - strand, within BWI76_RS24095 at 5026.807 kb on + strand, within BWI76_RS24095 at 5026.819 kb on + strand, within BWI76_RS24095 at 5026.909 kb on + strand, within BWI76_RS24095 at 5026.917 kb on - strand, within BWI76_RS24095 at 5027.021 kb on - strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.042 kb on - strand, within BWI76_RS24095 at 5027.100 kb on + strand, within BWI76_RS24095 at 5027.113 kb on + strand, within BWI76_RS24095 at 5027.135 kb on - strand, within BWI76_RS24095 at 5027.309 kb on + strand, within BWI76_RS24095 at 5027.340 kb on + strand, within BWI76_RS24095 at 5027.433 kb on + strand at 5027.446 kb on - strand at 5027.513 kb on - strand at 5027.513 kb on - strand at 5027.568 kb on + strand, within BWI76_RS24100 at 5027.838 kb on - strand, within BWI76_RS24100 at 5027.885 kb on + strand, within BWI76_RS24100 at 5027.888 kb on - strand, within BWI76_RS24100 at 5027.948 kb on - strand, within BWI76_RS24100 at 5027.963 kb on - strand, within BWI76_RS24100 at 5028.060 kb on - strand, within BWI76_RS24100 at 5028.132 kb on + strand at 5028.140 kb on - strand at 5028.140 kb on - strand at 5028.413 kb on + strand, within BWI76_RS24105 at 5028.413 kb on + strand, within BWI76_RS24105 at 5028.421 kb on - strand, within BWI76_RS24105 at 5028.421 kb on - strand, within BWI76_RS24105 at 5028.447 kb on + strand, within BWI76_RS24105 at 5028.447 kb on + strand, within BWI76_RS24105 at 5028.560 kb on + strand, within BWI76_RS24105 at 5028.723 kb on + strand at 5028.759 kb on + strand at 5028.824 kb on + strand, within BWI76_RS24110 at 5028.824 kb on + strand, within BWI76_RS24110 at 5029.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 5,026,490 + +0.4 5,026,490 + -0.7 5,026,495 - -0.0 5,026,495 - -0.6 5,026,496 - +0.0 5,026,498 + +0.0 5,026,512 - +0.4 5,026,513 - +0.1 5,026,515 + +0.1 5,026,515 + -0.2 5,026,515 + -0.2 5,026,515 + -0.8 5,026,515 + -0.3 5,026,523 - -0.3 5,026,523 - -0.0 5,026,523 - -0.0 5,026,523 - -0.6 5,026,523 - +0.3 5,026,523 - +0.3 5,026,544 + -0.4 5,026,544 + -0.2 5,026,544 + -0.7 5,026,544 + -0.5 5,026,544 + -0.4 5,026,563 + +0.4 5,026,571 - +0.2 5,026,586 + -0.3 5,026,594 - BWI76_RS24095 0.11 -0.2 5,026,612 - BWI76_RS24095 0.13 -0.1 5,026,622 - BWI76_RS24095 0.14 -0.5 5,026,624 - BWI76_RS24095 0.14 -0.6 5,026,637 - BWI76_RS24095 0.15 -1.7 5,026,647 - BWI76_RS24095 0.16 +0.5 5,026,657 - BWI76_RS24095 0.17 -0.5 5,026,667 + BWI76_RS24095 0.18 -0.1 5,026,776 + BWI76_RS24095 0.29 -0.1 5,026,784 - BWI76_RS24095 0.30 +0.2 5,026,807 + BWI76_RS24095 0.32 +0.3 5,026,819 + BWI76_RS24095 0.34 +0.1 5,026,909 + BWI76_RS24095 0.43 +0.3 5,026,917 - BWI76_RS24095 0.44 -0.4 5,027,021 - BWI76_RS24095 0.54 -0.4 5,027,034 + BWI76_RS24095 0.56 -0.3 5,027,034 + BWI76_RS24095 0.56 -0.4 5,027,034 + BWI76_RS24095 0.56 -0.7 5,027,034 + BWI76_RS24095 0.56 +0.2 5,027,042 - BWI76_RS24095 0.56 -0.0 5,027,100 + BWI76_RS24095 0.62 -0.5 5,027,113 + BWI76_RS24095 0.64 -0.2 5,027,135 - BWI76_RS24095 0.66 -1.5 5,027,309 + BWI76_RS24095 0.84 -0.6 5,027,340 + BWI76_RS24095 0.87 -0.3 5,027,433 + -0.2 5,027,446 - -0.8 5,027,513 - -1.5 5,027,513 - -0.4 5,027,568 + BWI76_RS24100 0.11 +0.4 5,027,838 - BWI76_RS24100 0.49 -0.2 5,027,885 + BWI76_RS24100 0.56 -0.4 5,027,888 - BWI76_RS24100 0.56 -1.5 5,027,948 - BWI76_RS24100 0.65 -1.1 5,027,963 - BWI76_RS24100 0.67 -1.3 5,028,060 - BWI76_RS24100 0.80 -1.9 5,028,132 + -0.4 5,028,140 - -0.9 5,028,140 - -0.1 5,028,413 + BWI76_RS24105 0.36 +0.2 5,028,413 + BWI76_RS24105 0.36 +0.4 5,028,421 - BWI76_RS24105 0.38 -0.3 5,028,421 - BWI76_RS24105 0.38 -0.1 5,028,447 + BWI76_RS24105 0.42 -0.0 5,028,447 + BWI76_RS24105 0.42 -0.4 5,028,560 + BWI76_RS24105 0.62 +0.2 5,028,723 + +0.1 5,028,759 + -1.3 5,028,824 + BWI76_RS24110 0.18 +0.7 5,028,824 + BWI76_RS24110 0.18 +0.2 5,029,038 - +0.6
Or see this region's nucleotide sequence