Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24065 and BWI76_RS24070 are separated by 90 nucleotides BWI76_RS24070 and BWI76_RS24075 are separated by 20 nucleotides BWI76_RS24075 and BWI76_RS24080 are separated by 176 nucleotides BWI76_RS24080 and BWI76_RS24085 overlap by 1 nucleotides
BWI76_RS24065: BWI76_RS24065 - deoxyribonuclease I, at 5,021,778 to 5,022,485
_RS24065
BWI76_RS24070: BWI76_RS24070 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 5,022,576 to 5,023,307
_RS24070
BWI76_RS24075: BWI76_RS24075 - glutathione synthase, at 5,023,328 to 5,024,275
_RS24075
BWI76_RS24080: BWI76_RS24080 - DUF179 domain-containing protein, at 5,024,452 to 5,025,015
_RS24080
BWI76_RS24085: BWI76_RS24085 - Holliday junction resolvase RuvX, at 5,025,015 to 5,025,431
_RS24085
Position (kb) 5023
5024
5025 Strain fitness (log2 ratio) -1
0
1 at 5022.393 kb on + strand, within BWI76_RS24065 at 5022.448 kb on - strand at 5022.678 kb on + strand, within BWI76_RS24070 at 5022.678 kb on + strand, within BWI76_RS24070 at 5022.827 kb on + strand, within BWI76_RS24070 at 5022.891 kb on - strand, within BWI76_RS24070 at 5023.064 kb on + strand, within BWI76_RS24070 at 5023.265 kb on - strand at 5023.310 kb on + strand at 5023.390 kb on - strand at 5023.483 kb on - strand, within BWI76_RS24075 at 5023.757 kb on - strand, within BWI76_RS24075 at 5023.773 kb on - strand, within BWI76_RS24075 at 5023.816 kb on - strand, within BWI76_RS24075 at 5023.821 kb on - strand, within BWI76_RS24075 at 5023.916 kb on + strand, within BWI76_RS24075 at 5024.250 kb on + strand at 5024.258 kb on - strand at 5024.309 kb on + strand at 5024.317 kb on - strand at 5024.521 kb on + strand, within BWI76_RS24080 at 5024.830 kb on + strand, within BWI76_RS24080 at 5024.835 kb on + strand, within BWI76_RS24080 at 5024.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 5,022,393 + BWI76_RS24065 0.87 +0.3 5,022,448 - +0.5 5,022,678 + BWI76_RS24070 0.14 +0.1 5,022,678 + BWI76_RS24070 0.14 +0.1 5,022,827 + BWI76_RS24070 0.34 -1.1 5,022,891 - BWI76_RS24070 0.43 +1.2 5,023,064 + BWI76_RS24070 0.67 -0.4 5,023,265 - -1.4 5,023,310 + +0.5 5,023,390 - -1.3 5,023,483 - BWI76_RS24075 0.16 +0.2 5,023,757 - BWI76_RS24075 0.45 -1.6 5,023,773 - BWI76_RS24075 0.47 +0.1 5,023,816 - BWI76_RS24075 0.51 -1.3 5,023,821 - BWI76_RS24075 0.52 -1.6 5,023,916 + BWI76_RS24075 0.62 -1.6 5,024,250 + -0.5 5,024,258 - -0.6 5,024,309 + +0.8 5,024,317 - +0.5 5,024,521 + BWI76_RS24080 0.12 -0.1 5,024,830 + BWI76_RS24080 0.67 -1.7 5,024,835 + BWI76_RS24080 0.68 +0.4 5,024,971 + +0.6
Or see this region's nucleotide sequence