Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24055 and BWI76_RS24060 are separated by 75 nucleotides BWI76_RS24060 and BWI76_RS24065 are separated by 94 nucleotides BWI76_RS24065 and BWI76_RS24070 are separated by 90 nucleotides BWI76_RS24070 and BWI76_RS24075 are separated by 20 nucleotides
BWI76_RS24055: BWI76_RS24055 - MFS transporter, at 5,019,713 to 5,021,107
_RS24055
BWI76_RS24060: BWI76_RS24060 - SprT family protein, at 5,021,183 to 5,021,683
_RS24060
BWI76_RS24065: BWI76_RS24065 - deoxyribonuclease I, at 5,021,778 to 5,022,485
_RS24065
BWI76_RS24070: BWI76_RS24070 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 5,022,576 to 5,023,307
_RS24070
BWI76_RS24075: BWI76_RS24075 - glutathione synthase, at 5,023,328 to 5,024,275
_RS24075
Position (kb) 5021
5022
5023 Strain fitness (log2 ratio) -1
0
1 at 5020.807 kb on - strand, within BWI76_RS24055 at 5021.081 kb on - strand at 5021.275 kb on + strand, within BWI76_RS24060 at 5021.283 kb on - strand, within BWI76_RS24060 at 5021.769 kb on + strand at 5021.875 kb on - strand, within BWI76_RS24065 at 5022.161 kb on + strand, within BWI76_RS24065 at 5022.161 kb on + strand, within BWI76_RS24065 at 5022.393 kb on + strand, within BWI76_RS24065 at 5022.448 kb on - strand at 5022.678 kb on + strand, within BWI76_RS24070 at 5022.678 kb on + strand, within BWI76_RS24070 at 5022.827 kb on + strand, within BWI76_RS24070 at 5022.891 kb on - strand, within BWI76_RS24070 at 5023.064 kb on + strand, within BWI76_RS24070 at 5023.265 kb on - strand at 5023.310 kb on + strand at 5023.390 kb on - strand at 5023.483 kb on - strand, within BWI76_RS24075
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 5,020,807 - BWI76_RS24055 0.78 -1.3 5,021,081 - +0.8 5,021,275 + BWI76_RS24060 0.18 -0.1 5,021,283 - BWI76_RS24060 0.20 +0.0 5,021,769 + -0.2 5,021,875 - BWI76_RS24065 0.14 +0.6 5,022,161 + BWI76_RS24065 0.54 -0.1 5,022,161 + BWI76_RS24065 0.54 +0.6 5,022,393 + BWI76_RS24065 0.87 +0.3 5,022,448 - +0.5 5,022,678 + BWI76_RS24070 0.14 +0.1 5,022,678 + BWI76_RS24070 0.14 +0.1 5,022,827 + BWI76_RS24070 0.34 -1.1 5,022,891 - BWI76_RS24070 0.43 +1.2 5,023,064 + BWI76_RS24070 0.67 -0.4 5,023,265 - -1.4 5,023,310 + +0.5 5,023,390 - -1.3 5,023,483 - BWI76_RS24075 0.16 +0.2
Or see this region's nucleotide sequence