Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22530

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22525 and BWI76_RS22530 overlap by 4 nucleotidesBWI76_RS22530 and BWI76_RS22535 overlap by 1 nucleotides BWI76_RS22525: BWI76_RS22525 - cation efflux system protein, at 4,694,719 to 4,696,116 _RS22525 BWI76_RS22530: BWI76_RS22530 - macrolide ABC transporter permease/ATP-binding protein MacB, at 4,696,113 to 4,698,056 _RS22530 BWI76_RS22535: BWI76_RS22535 - membrane fusion protein of a type I secretion system, at 4,698,056 to 4,699,216 _RS22535 Position (kb) 4696 4697 4698 4699Strain fitness (log2 ratio) -1 0 1at 4695.352 kb on + strand, within BWI76_RS22525at 4695.426 kb on + strand, within BWI76_RS22525at 4695.610 kb on - strand, within BWI76_RS22525at 4695.655 kb on - strand, within BWI76_RS22525at 4695.813 kb on - strand, within BWI76_RS22525at 4695.832 kb on + strand, within BWI76_RS22525at 4695.864 kb on + strand, within BWI76_RS22525at 4695.870 kb on + strand, within BWI76_RS22525at 4695.870 kb on + strand, within BWI76_RS22525at 4696.110 kb on - strandat 4696.246 kb on - strandat 4696.251 kb on - strandat 4696.279 kb on - strandat 4696.491 kb on - strand, within BWI76_RS22530at 4696.530 kb on - strand, within BWI76_RS22530at 4696.530 kb on - strand, within BWI76_RS22530at 4696.904 kb on - strand, within BWI76_RS22530at 4697.212 kb on + strand, within BWI76_RS22530at 4697.371 kb on + strand, within BWI76_RS22530at 4697.526 kb on + strand, within BWI76_RS22530at 4697.637 kb on - strand, within BWI76_RS22530at 4697.759 kb on - strand, within BWI76_RS22530at 4697.800 kb on - strand, within BWI76_RS22530at 4698.030 kb on + strandat 4698.171 kb on - strandat 4698.195 kb on + strand, within BWI76_RS22535at 4698.260 kb on - strand, within BWI76_RS22535at 4698.260 kb on - strand, within BWI76_RS22535at 4698.356 kb on + strand, within BWI76_RS22535at 4698.477 kb on - strand, within BWI76_RS22535at 4698.536 kb on - strand, within BWI76_RS22535at 4698.536 kb on - strand, within BWI76_RS22535at 4698.625 kb on + strand, within BWI76_RS22535at 4698.668 kb on - strand, within BWI76_RS22535at 4698.671 kb on + strand, within BWI76_RS22535at 4698.764 kb on - strand, within BWI76_RS22535at 4698.766 kb on + strand, within BWI76_RS22535at 4698.784 kb on + strand, within BWI76_RS22535at 4698.784 kb on + strand, within BWI76_RS22535at 4698.784 kb on + strand, within BWI76_RS22535at 4698.792 kb on - strand, within BWI76_RS22535at 4698.792 kb on - strand, within BWI76_RS22535at 4698.826 kb on - strand, within BWI76_RS22535at 4698.832 kb on - strand, within BWI76_RS22535at 4698.952 kb on - strand, within BWI76_RS22535at 4698.999 kb on + strand, within BWI76_RS22535at 4698.999 kb on + strand, within BWI76_RS22535

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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4,695,352 + BWI76_RS22525 0.45 +0.1
4,695,426 + BWI76_RS22525 0.51 -0.5
4,695,610 - BWI76_RS22525 0.64 -0.3
4,695,655 - BWI76_RS22525 0.67 -0.5
4,695,813 - BWI76_RS22525 0.78 +0.2
4,695,832 + BWI76_RS22525 0.80 -0.1
4,695,864 + BWI76_RS22525 0.82 -0.5
4,695,870 + BWI76_RS22525 0.82 +0.0
4,695,870 + BWI76_RS22525 0.82 +0.4
4,696,110 - +0.4
4,696,246 - -0.0
4,696,251 - -0.3
4,696,279 - +0.2
4,696,491 - BWI76_RS22530 0.19 +0.2
4,696,530 - BWI76_RS22530 0.21 -0.4
4,696,530 - BWI76_RS22530 0.21 +0.4
4,696,904 - BWI76_RS22530 0.41 -0.1
4,697,212 + BWI76_RS22530 0.57 +0.1
4,697,371 + BWI76_RS22530 0.65 +0.9
4,697,526 + BWI76_RS22530 0.73 +0.0
4,697,637 - BWI76_RS22530 0.78 +0.1
4,697,759 - BWI76_RS22530 0.85 +0.8
4,697,800 - BWI76_RS22530 0.87 +0.7
4,698,030 + +0.7
4,698,171 - -0.4
4,698,195 + BWI76_RS22535 0.12 -0.6
4,698,260 - BWI76_RS22535 0.18 +0.0
4,698,260 - BWI76_RS22535 0.18 -0.3
4,698,356 + BWI76_RS22535 0.26 +0.7
4,698,477 - BWI76_RS22535 0.36 -0.4
4,698,536 - BWI76_RS22535 0.41 +0.1
4,698,536 - BWI76_RS22535 0.41 +0.4
4,698,625 + BWI76_RS22535 0.49 -0.1
4,698,668 - BWI76_RS22535 0.53 -0.3
4,698,671 + BWI76_RS22535 0.53 +0.1
4,698,764 - BWI76_RS22535 0.61 -0.0
4,698,766 + BWI76_RS22535 0.61 +0.4
4,698,784 + BWI76_RS22535 0.63 -0.3
4,698,784 + BWI76_RS22535 0.63 -0.9
4,698,784 + BWI76_RS22535 0.63 -1.1
4,698,792 - BWI76_RS22535 0.63 -0.4
4,698,792 - BWI76_RS22535 0.63 -0.2
4,698,826 - BWI76_RS22535 0.66 -0.2
4,698,832 - BWI76_RS22535 0.67 -0.7
4,698,952 - BWI76_RS22535 0.77 -0.3
4,698,999 + BWI76_RS22535 0.81 -0.3
4,698,999 + BWI76_RS22535 0.81 +1.3

Or see this region's nucleotide sequence