Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20285 and BWI76_RS20290 are separated by 208 nucleotides BWI76_RS20290 and BWI76_RS20295 are separated by 65 nucleotides BWI76_RS20295 and BWI76_RS20300 are separated by 23 nucleotides
BWI76_RS20285: BWI76_RS20285 - glutathione S-transferase, at 4,250,307 to 4,250,951
_RS20285
BWI76_RS20290: BWI76_RS20290 - thiol:disulfide oxidoreductase, at 4,251,160 to 4,251,789
_RS20290
BWI76_RS20295: BWI76_RS20295 - dihydroneopterin triphosphate 2'-epimerase, at 4,251,855 to 4,252,217
_RS20295
BWI76_RS20300: BWI76_RS20300 - epimerase, at 4,252,241 to 4,253,134
_RS20300
Position (kb) 4251
4252
4253 Strain fitness (log2 ratio) -2
-1
0
1 at 4250.946 kb on + strand at 4251.047 kb on + strand at 4251.055 kb on - strand at 4251.062 kb on - strand at 4251.082 kb on + strand at 4251.261 kb on - strand, within BWI76_RS20290 at 4251.364 kb on - strand, within BWI76_RS20290 at 4251.451 kb on - strand, within BWI76_RS20290 at 4251.611 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.847 kb on + strand at 4252.004 kb on + strand, within BWI76_RS20295 at 4252.130 kb on + strand, within BWI76_RS20295 at 4252.172 kb on + strand, within BWI76_RS20295 at 4252.172 kb on + strand, within BWI76_RS20295 at 4252.180 kb on - strand, within BWI76_RS20295
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 4,250,946 + -0.2 4,251,047 + +0.7 4,251,055 - +0.0 4,251,062 - -0.4 4,251,082 + -0.6 4,251,261 - BWI76_RS20290 0.16 -1.8 4,251,364 - BWI76_RS20290 0.32 +0.2 4,251,451 - BWI76_RS20290 0.46 -0.3 4,251,611 + BWI76_RS20290 0.72 -1.0 4,251,715 + BWI76_RS20290 0.88 -0.2 4,251,715 + BWI76_RS20290 0.88 -0.5 4,251,847 + -0.1 4,252,004 + BWI76_RS20295 0.41 +0.7 4,252,130 + BWI76_RS20295 0.76 +0.6 4,252,172 + BWI76_RS20295 0.87 +0.3 4,252,172 + BWI76_RS20295 0.87 +0.0 4,252,180 - BWI76_RS20295 0.90 -0.4
Or see this region's nucleotide sequence