Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20115

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20110 and BWI76_RS20115 are separated by 30 nucleotidesBWI76_RS20115 and BWI76_RS20120 are separated by 62 nucleotides BWI76_RS20110: BWI76_RS20110 - fimbrial assembly protein, at 4,211,975 to 4,214,524 _RS20110 BWI76_RS20115: BWI76_RS20115 - hypothetical protein, at 4,214,555 to 4,215,217 _RS20115 BWI76_RS20120: BWI76_RS20120 - fimbrial protein, at 4,215,280 to 4,215,807 _RS20120 Position (kb) 4214 4215 4216Strain fitness (log2 ratio) -1 0 1 2at 4213.669 kb on + strand, within BWI76_RS20110at 4213.828 kb on - strand, within BWI76_RS20110at 4214.046 kb on + strand, within BWI76_RS20110at 4214.046 kb on + strand, within BWI76_RS20110at 4214.046 kb on + strand, within BWI76_RS20110at 4214.157 kb on - strand, within BWI76_RS20110at 4214.334 kb on + strandat 4214.445 kb on + strandat 4214.453 kb on - strandat 4214.536 kb on - strandat 4214.545 kb on - strandat 4214.550 kb on - strandat 4214.573 kb on - strandat 4214.628 kb on - strand, within BWI76_RS20115at 4214.880 kb on + strand, within BWI76_RS20115at 4214.880 kb on + strand, within BWI76_RS20115at 4214.888 kb on - strandat 4215.066 kb on - strand, within BWI76_RS20115at 4215.070 kb on + strand, within BWI76_RS20115at 4215.402 kb on + strand, within BWI76_RS20120at 4215.414 kb on - strand, within BWI76_RS20120at 4215.479 kb on + strand, within BWI76_RS20120at 4215.487 kb on - strand, within BWI76_RS20120at 4215.668 kb on + strand, within BWI76_RS20120at 4215.676 kb on - strand, within BWI76_RS20120at 4215.730 kb on - strand, within BWI76_RS20120at 4215.730 kb on - strand, within BWI76_RS20120at 4215.734 kb on + strand, within BWI76_RS20120at 4215.745 kb on - strand, within BWI76_RS20120at 4215.745 kb on - strand, within BWI76_RS20120at 4215.745 kb on - strand, within BWI76_RS20120at 4215.747 kb on + strand, within BWI76_RS20120at 4215.747 kb on + strand, within BWI76_RS20120at 4215.747 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.752 kb on + strand, within BWI76_RS20120at 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.760 kb on - strandat 4215.772 kb on + strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.780 kb on - strandat 4215.788 kb on - strandat 4215.818 kb on + strandat 4215.818 kb on + strandat 4215.826 kb on - strandat 4215.848 kb on + strandat 4215.848 kb on + strandat 4215.848 kb on + strandat 4215.848 kb on + strandat 4215.848 kb on + strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.856 kb on - strandat 4215.885 kb on + strandat 4215.952 kb on + strandat 4216.028 kb on - strandat 4216.073 kb on - strandat 4216.074 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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4,213,669 + BWI76_RS20110 0.66 -0.2
4,213,828 - BWI76_RS20110 0.73 -0.2
4,214,046 + BWI76_RS20110 0.81 +0.0
4,214,046 + BWI76_RS20110 0.81 -0.3
4,214,046 + BWI76_RS20110 0.81 -0.8
4,214,157 - BWI76_RS20110 0.86 -0.2
4,214,334 + -0.2
4,214,445 + +1.8
4,214,453 - +1.1
4,214,536 - +0.3
4,214,545 - -1.4
4,214,550 - +0.2
4,214,573 - -0.4
4,214,628 - BWI76_RS20115 0.11 +0.5
4,214,880 + BWI76_RS20115 0.49 +1.0
4,214,880 + BWI76_RS20115 0.49 +0.1
4,214,888 - -0.8
4,215,066 - BWI76_RS20115 0.77 +0.2
4,215,070 + BWI76_RS20115 0.78 -0.2
4,215,402 + BWI76_RS20120 0.23 -0.1
4,215,414 - BWI76_RS20120 0.25 -0.5
4,215,479 + BWI76_RS20120 0.38 -0.0
4,215,487 - BWI76_RS20120 0.39 +0.0
4,215,668 + BWI76_RS20120 0.73 +0.0
4,215,676 - BWI76_RS20120 0.75 +0.4
4,215,730 - BWI76_RS20120 0.85 -0.2
4,215,730 - BWI76_RS20120 0.85 -0.7
4,215,734 + BWI76_RS20120 0.86 +0.6
4,215,745 - BWI76_RS20120 0.88 -0.6
4,215,745 - BWI76_RS20120 0.88 -0.0
4,215,745 - BWI76_RS20120 0.88 -0.7
4,215,747 + BWI76_RS20120 0.88 +0.3
4,215,747 + BWI76_RS20120 0.88 -0.6
4,215,747 + BWI76_RS20120 0.88 +0.5
4,215,752 + BWI76_RS20120 0.89 +1.4
4,215,752 + BWI76_RS20120 0.89 +0.3
4,215,752 + BWI76_RS20120 0.89 -0.2
4,215,752 + BWI76_RS20120 0.89 -0.3
4,215,752 + BWI76_RS20120 0.89 -0.4
4,215,752 + BWI76_RS20120 0.89 -0.3
4,215,752 + BWI76_RS20120 0.89 +0.3
4,215,760 - +0.2
4,215,760 - -0.0
4,215,760 - -0.4
4,215,760 - +0.0
4,215,760 - -0.2
4,215,760 - +0.3
4,215,760 - +0.6
4,215,760 - +0.6
4,215,760 - -0.2
4,215,760 - -0.5
4,215,772 + +0.6
4,215,780 - +0.4
4,215,780 - -0.0
4,215,780 - -0.3
4,215,780 - +0.5
4,215,780 - -0.8
4,215,780 - -0.8
4,215,780 - +0.0
4,215,780 - -0.3
4,215,788 - -0.3
4,215,818 + -0.2
4,215,818 + +0.0
4,215,826 - +0.1
4,215,848 + -0.2
4,215,848 + -0.0
4,215,848 + -0.5
4,215,848 + -0.4
4,215,848 + -0.5
4,215,856 - -0.5
4,215,856 - +0.1
4,215,856 - -0.5
4,215,856 - -0.1
4,215,856 - -0.9
4,215,856 - +0.1
4,215,856 - -0.1
4,215,885 + +0.3
4,215,952 + +0.6
4,216,028 - +1.1
4,216,073 - -0.2
4,216,074 - -0.2

Or see this region's nucleotide sequence