Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17205

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17190 and BWI76_RS17195 are separated by 211 nucleotidesBWI76_RS17195 and BWI76_RS17200 are separated by 58 nucleotidesBWI76_RS17200 and BWI76_RS17205 are separated by 32 nucleotidesBWI76_RS17205 and BWI76_RS17210 are separated by 34 nucleotidesBWI76_RS17210 and BWI76_RS17215 are separated by 67 nucleotides BWI76_RS17190: BWI76_RS17190 - TonB system transport protein TonB, at 3,562,317 to 3,563,054 _RS17190 BWI76_RS17195: BWI76_RS17195 - hypothetical protein, at 3,563,266 to 3,563,562 _RS17195 BWI76_RS17200: BWI76_RS17200 - hypothetical protein, at 3,563,621 to 3,563,905 _RS17200 BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398 _RS17205 BWI76_RS17210: BWI76_RS17210 - dsDNA-mimic protein, at 3,565,433 to 3,565,762 _RS17210 BWI76_RS17215: BWI76_RS17215 - transporter, at 3,565,830 to 3,566,667 _RS17215 Position (kb) 3563 3564 3565 3566Strain fitness (log2 ratio) -2 -1 0 1at 3562.960 kb on - strand, within BWI76_RS17190at 3562.965 kb on - strand, within BWI76_RS17190at 3563.466 kb on + strand, within BWI76_RS17195at 3563.639 kb on + strandat 3563.776 kb on - strand, within BWI76_RS17200at 3563.964 kb on - strandat 3563.964 kb on - strandat 3563.978 kb on - strandat 3564.296 kb on + strand, within BWI76_RS17205at 3564.342 kb on - strand, within BWI76_RS17205at 3564.389 kb on - strand, within BWI76_RS17205at 3564.598 kb on - strand, within BWI76_RS17205at 3564.913 kb on - strand, within BWI76_RS17205at 3565.201 kb on - strand, within BWI76_RS17205at 3565.325 kb on + strandat 3565.400 kb on - strandat 3565.600 kb on - strand, within BWI76_RS17210at 3565.602 kb on + strand, within BWI76_RS17210at 3565.607 kb on - strand, within BWI76_RS17210at 3565.629 kb on + strand, within BWI76_RS17210at 3565.658 kb on + strand, within BWI76_RS17210at 3565.702 kb on - strand, within BWI76_RS17210at 3565.735 kb on - strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.740 kb on + strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.748 kb on - strandat 3565.798 kb on - strandat 3565.841 kb on + strandat 3565.908 kb on + strandat 3565.916 kb on + strand, within BWI76_RS17215at 3565.916 kb on + strand, within BWI76_RS17215at 3565.936 kb on + strand, within BWI76_RS17215at 3566.006 kb on - strand, within BWI76_RS17215at 3566.034 kb on - strand, within BWI76_RS17215at 3566.197 kb on + strand, within BWI76_RS17215at 3566.198 kb on - strand, within BWI76_RS17215at 3566.207 kb on + strand, within BWI76_RS17215at 3566.210 kb on - strand, within BWI76_RS17215at 3566.215 kb on - strand, within BWI76_RS17215at 3566.220 kb on - strand, within BWI76_RS17215at 3566.244 kb on + strand, within BWI76_RS17215at 3566.259 kb on + strand, within BWI76_RS17215at 3566.321 kb on - strand, within BWI76_RS17215at 3566.323 kb on + strand, within BWI76_RS17215at 3566.325 kb on + strand, within BWI76_RS17215at 3566.326 kb on - strand, within BWI76_RS17215at 3566.345 kb on + strand, within BWI76_RS17215

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,562,960 - BWI76_RS17190 0.87 -1.3
3,562,965 - BWI76_RS17190 0.88 -0.6
3,563,466 + BWI76_RS17195 0.67 -0.4
3,563,639 + +0.9
3,563,776 - BWI76_RS17200 0.54 +0.3
3,563,964 - -0.2
3,563,964 - +0.9
3,563,978 - +1.1
3,564,296 + BWI76_RS17205 0.25 +0.4
3,564,342 - BWI76_RS17205 0.28 +0.3
3,564,389 - BWI76_RS17205 0.31 +0.5
3,564,598 - BWI76_RS17205 0.45 +0.3
3,564,913 - BWI76_RS17205 0.67 +1.0
3,565,201 - BWI76_RS17205 0.86 -1.4
3,565,325 + +1.4
3,565,400 - +0.2
3,565,600 - BWI76_RS17210 0.51 -0.1
3,565,602 + BWI76_RS17210 0.51 -0.1
3,565,607 - BWI76_RS17210 0.53 -0.7
3,565,629 + BWI76_RS17210 0.59 -0.1
3,565,658 + BWI76_RS17210 0.68 +0.5
3,565,702 - BWI76_RS17210 0.82 +0.3
3,565,735 - -0.0
3,565,740 + +0.1
3,565,740 + +0.0
3,565,740 + -0.0
3,565,740 + -1.4
3,565,740 + -0.1
3,565,740 + +0.2
3,565,740 + -0.0
3,565,740 + +0.0
3,565,740 + +0.4
3,565,740 + +0.0
3,565,740 + -0.5
3,565,740 + -0.0
3,565,740 + -0.8
3,565,748 - -0.5
3,565,748 - -0.8
3,565,748 - +0.4
3,565,748 - -0.5
3,565,748 - +0.1
3,565,748 - -1.0
3,565,798 - -1.0
3,565,841 + -0.5
3,565,908 + +0.2
3,565,916 + BWI76_RS17215 0.10 -0.5
3,565,916 + BWI76_RS17215 0.10 -0.1
3,565,936 + BWI76_RS17215 0.13 -0.1
3,566,006 - BWI76_RS17215 0.21 -0.0
3,566,034 - BWI76_RS17215 0.24 -0.4
3,566,197 + BWI76_RS17215 0.44 +0.7
3,566,198 - BWI76_RS17215 0.44 -0.1
3,566,207 + BWI76_RS17215 0.45 -0.0
3,566,210 - BWI76_RS17215 0.45 -2.0
3,566,215 - BWI76_RS17215 0.46 -0.6
3,566,220 - BWI76_RS17215 0.47 +0.2
3,566,244 + BWI76_RS17215 0.49 +0.3
3,566,259 + BWI76_RS17215 0.51 +0.2
3,566,321 - BWI76_RS17215 0.59 -0.8
3,566,323 + BWI76_RS17215 0.59 -0.1
3,566,325 + BWI76_RS17215 0.59 +0.6
3,566,326 - BWI76_RS17215 0.59 -0.0
3,566,345 + BWI76_RS17215 0.61 -0.8

Or see this region's nucleotide sequence