Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17105 and BWI76_RS17110 are separated by 24 nucleotides BWI76_RS17110 and BWI76_RS17115 are separated by 96 nucleotides BWI76_RS17115 and BWI76_RS17120 are separated by 4 nucleotides
BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635
_RS17105
BWI76_RS17110: BWI76_RS17110 - vitamin B12 ABC transporter permease BtuC, at 3,549,660 to 3,550,646
_RS17110
BWI76_RS17115: BWI76_RS17115 - integration host factor subunit alpha, at 3,550,743 to 3,551,042
_RS17115
BWI76_RS17120: BWI76_RS17120 - phenylalanine--tRNA ligase subunit beta, at 3,551,047 to 3,553,434
_RS17120
Position (kb)
3549
3550
3551 Strain fitness (log2 ratio)
-1
0
1 at 3548.743 kb on - strand at 3548.856 kb on + strand at 3548.868 kb on + strand at 3548.882 kb on + strand at 3548.890 kb on - strand at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.091 kb on + strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.137 kb on + strand, within BWI76_RS17105 at 3549.375 kb on + strand, within BWI76_RS17105 at 3549.378 kb on - strand, within BWI76_RS17105 at 3549.382 kb on + strand, within BWI76_RS17105 at 3549.383 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.441 kb on - strand at 3549.446 kb on - strand, within BWI76_RS17105 at 3549.492 kb on - strand, within BWI76_RS17105 at 3549.613 kb on - strand at 3549.713 kb on + strand at 3550.305 kb on + strand, within BWI76_RS17110 at 3550.595 kb on - strand at 3550.638 kb on + strand at 3551.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,548,743 - -0.5 3,548,856 + -0.2 3,548,868 + -0.3 3,548,882 + +0.0 3,548,890 - +0.2 3,549,086 + BWI76_RS17105 0.31 +0.1 3,549,086 + BWI76_RS17105 0.31 -0.0 3,549,091 + BWI76_RS17105 0.32 -0.3 3,549,094 - BWI76_RS17105 0.32 +0.4 3,549,094 - BWI76_RS17105 0.32 -0.1 3,549,137 + BWI76_RS17105 0.38 -0.3 3,549,375 + BWI76_RS17105 0.67 -0.0 3,549,378 - BWI76_RS17105 0.68 -0.2 3,549,382 + BWI76_RS17105 0.68 +0.4 3,549,383 - BWI76_RS17105 0.68 -0.2 3,549,390 - BWI76_RS17105 0.69 -0.4 3,549,390 - BWI76_RS17105 0.69 -0.0 3,549,441 - +1.5 3,549,446 - BWI76_RS17105 0.76 -0.7 3,549,492 - BWI76_RS17105 0.82 +0.0 3,549,613 - +0.3 3,549,713 + -0.8 3,550,305 + BWI76_RS17110 0.65 -1.3 3,550,595 - -1.3 3,550,638 + -0.7 3,551,047 - -0.0
Or see this region's nucleotide sequence