Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16315

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16310 and BWI76_RS16315 overlap by 4 nucleotidesBWI76_RS16315 and BWI76_RS16320 are separated by 77 nucleotides BWI76_RS16310: BWI76_RS16310 - hypothetical protein, at 3,386,065 to 3,387,276 _RS16310 BWI76_RS16315: BWI76_RS16315 - SirA-like protein, at 3,387,273 to 3,387,506 _RS16315 BWI76_RS16320: BWI76_RS16320 - methionine ABC transporter substrate-binding protein, at 3,387,584 to 3,388,396 _RS16320 Position (kb) 3387 3388Strain fitness (log2 ratio) -1 0 1at 3386.295 kb on - strand, within BWI76_RS16310at 3386.333 kb on - strand, within BWI76_RS16310at 3386.414 kb on - strand, within BWI76_RS16310at 3386.562 kb on - strand, within BWI76_RS16310at 3387.081 kb on + strand, within BWI76_RS16310at 3387.098 kb on + strand, within BWI76_RS16310at 3387.098 kb on + strand, within BWI76_RS16310at 3387.143 kb on + strand, within BWI76_RS16310at 3387.445 kb on + strand, within BWI76_RS16315at 3387.455 kb on + strand, within BWI76_RS16315at 3388.085 kb on - strand, within BWI76_RS16320at 3388.101 kb on - strand, within BWI76_RS16320at 3388.101 kb on - strandat 3388.136 kb on + strand, within BWI76_RS16320at 3388.187 kb on - strand, within BWI76_RS16320at 3388.187 kb on - strand, within BWI76_RS16320at 3388.187 kb on - strand, within BWI76_RS16320at 3388.219 kb on + strand, within BWI76_RS16320at 3388.314 kb on - strand, within BWI76_RS16320at 3388.500 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,386,295 - BWI76_RS16310 0.19 -0.1
3,386,333 - BWI76_RS16310 0.22 +0.1
3,386,414 - BWI76_RS16310 0.29 +0.8
3,386,562 - BWI76_RS16310 0.41 +0.5
3,387,081 + BWI76_RS16310 0.84 +0.5
3,387,098 + BWI76_RS16310 0.85 +0.0
3,387,098 + BWI76_RS16310 0.85 +0.0
3,387,143 + BWI76_RS16310 0.89 -0.2
3,387,445 + BWI76_RS16315 0.74 -1.1
3,387,455 + BWI76_RS16315 0.78 -0.4
3,388,085 - BWI76_RS16320 0.62 +0.4
3,388,101 - BWI76_RS16320 0.64 -1.0
3,388,101 - +0.7
3,388,136 + BWI76_RS16320 0.68 +0.3
3,388,187 - BWI76_RS16320 0.74 -0.9
3,388,187 - BWI76_RS16320 0.74 -0.0
3,388,187 - BWI76_RS16320 0.74 -0.2
3,388,219 + BWI76_RS16320 0.78 +0.3
3,388,314 - BWI76_RS16320 0.90 -0.3
3,388,500 + -1.0

Or see this region's nucleotide sequence