Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16300 and BWI76_RS16305 are separated by 315 nucleotides BWI76_RS16305 and BWI76_RS16310 are separated by 188 nucleotides BWI76_RS16310 and BWI76_RS16315 overlap by 4 nucleotides BWI76_RS16315 and BWI76_RS16320 are separated by 77 nucleotides
BWI76_RS16300: BWI76_RS16300 - bifunctional beta-cystathionase/maltose regulon regulatory protein, at 3,384,179 to 3,385,357
_RS16300
BWI76_RS16305: BWI76_RS16305 - selenium-binding protein YdfZ, at 3,385,673 to 3,385,876
_RS16305
BWI76_RS16310: BWI76_RS16310 - hypothetical protein, at 3,386,065 to 3,387,276
_RS16310
BWI76_RS16315: BWI76_RS16315 - SirA-like protein, at 3,387,273 to 3,387,506
_RS16315
BWI76_RS16320: BWI76_RS16320 - methionine ABC transporter substrate-binding protein, at 3,387,584 to 3,388,396
_RS16320
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-1
0
1 at 3385.327 kb on - strand at 3385.372 kb on + strand at 3385.372 kb on + strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.474 kb on + strand at 3385.539 kb on + strand at 3385.547 kb on - strand at 3385.574 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.699 kb on + strand, within BWI76_RS16305 at 3385.701 kb on - strand, within BWI76_RS16305 at 3385.727 kb on - strand, within BWI76_RS16305 at 3385.727 kb on - strand, within BWI76_RS16305 at 3385.742 kb on - strand, within BWI76_RS16305 at 3385.751 kb on - strand, within BWI76_RS16305 at 3385.761 kb on - strand, within BWI76_RS16305 at 3385.774 kb on + strand, within BWI76_RS16305 at 3385.782 kb on - strand, within BWI76_RS16305 at 3385.787 kb on + strand at 3385.787 kb on + strand, within BWI76_RS16305 at 3385.792 kb on + strand, within BWI76_RS16305 at 3385.792 kb on + strand, within BWI76_RS16305 at 3385.795 kb on - strand, within BWI76_RS16305 at 3385.865 kb on + strand at 3385.865 kb on + strand at 3385.865 kb on + strand at 3385.867 kb on - strand at 3385.880 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.899 kb on - strand at 3385.899 kb on - strand at 3385.916 kb on + strand at 3385.924 kb on + strand at 3385.925 kb on + strand at 3385.925 kb on + strand at 3385.927 kb on + strand at 3385.932 kb on - strand at 3385.934 kb on + strand at 3385.937 kb on - strand at 3385.983 kb on - strand at 3386.031 kb on + strand at 3386.033 kb on + strand at 3386.069 kb on + strand at 3386.069 kb on - strand at 3386.069 kb on - strand at 3386.079 kb on + strand at 3386.086 kb on - strand at 3386.087 kb on - strand at 3386.087 kb on - strand at 3386.143 kb on + strand at 3386.151 kb on - strand at 3386.157 kb on - strand at 3386.157 kb on - strand at 3386.243 kb on + strand, within BWI76_RS16310 at 3386.266 kb on - strand at 3386.295 kb on - strand, within BWI76_RS16310 at 3386.333 kb on - strand, within BWI76_RS16310 at 3386.414 kb on - strand, within BWI76_RS16310 at 3386.562 kb on - strand, within BWI76_RS16310 at 3387.081 kb on + strand, within BWI76_RS16310 at 3387.098 kb on + strand, within BWI76_RS16310 at 3387.098 kb on + strand, within BWI76_RS16310 at 3387.143 kb on + strand, within BWI76_RS16310 at 3387.445 kb on + strand, within BWI76_RS16315 at 3387.455 kb on + strand, within BWI76_RS16315 at 3388.085 kb on - strand, within BWI76_RS16320 at 3388.101 kb on - strand, within BWI76_RS16320 at 3388.101 kb on - strand at 3388.136 kb on + strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.219 kb on + strand, within BWI76_RS16320
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,385,327 - +0.3 3,385,372 + +0.3 3,385,372 + -0.1 3,385,380 - -0.3 3,385,380 - -0.6 3,385,380 - +0.1 3,385,380 - -0.5 3,385,474 + +0.1 3,385,539 + +0.0 3,385,547 - -1.0 3,385,574 + +0.1 3,385,693 + -0.5 3,385,693 + +0.8 3,385,693 + -0.0 3,385,693 + -1.1 3,385,699 + BWI76_RS16305 0.13 -0.2 3,385,701 - BWI76_RS16305 0.14 -0.4 3,385,727 - BWI76_RS16305 0.26 -0.0 3,385,727 - BWI76_RS16305 0.26 +0.1 3,385,742 - BWI76_RS16305 0.34 +0.1 3,385,751 - BWI76_RS16305 0.38 -0.4 3,385,761 - BWI76_RS16305 0.43 -0.1 3,385,774 + BWI76_RS16305 0.50 -0.6 3,385,782 - BWI76_RS16305 0.53 +0.5 3,385,787 + +1.5 3,385,787 + BWI76_RS16305 0.56 +0.1 3,385,792 + BWI76_RS16305 0.58 -0.5 3,385,792 + BWI76_RS16305 0.58 +0.3 3,385,795 - BWI76_RS16305 0.60 +0.5 3,385,865 + -0.4 3,385,865 + -0.3 3,385,865 + -0.1 3,385,867 - -0.3 3,385,880 + -0.1 3,385,891 + -0.5 3,385,891 + -0.6 3,385,891 + -0.4 3,385,891 + +0.2 3,385,899 - +0.5 3,385,899 - -0.7 3,385,916 + -0.5 3,385,924 + +0.5 3,385,925 + -0.2 3,385,925 + +0.3 3,385,927 + +0.0 3,385,932 - -0.0 3,385,934 + -0.5 3,385,937 - -1.5 3,385,983 - -0.4 3,386,031 + +0.3 3,386,033 + -0.5 3,386,069 + -0.7 3,386,069 - +1.0 3,386,069 - -0.2 3,386,079 + -0.8 3,386,086 - -0.9 3,386,087 - +0.0 3,386,087 - -0.0 3,386,143 + -0.4 3,386,151 - -0.3 3,386,157 - -0.1 3,386,157 - -0.4 3,386,243 + BWI76_RS16310 0.15 +0.4 3,386,266 - +1.1 3,386,295 - BWI76_RS16310 0.19 -0.1 3,386,333 - BWI76_RS16310 0.22 +0.1 3,386,414 - BWI76_RS16310 0.29 +0.8 3,386,562 - BWI76_RS16310 0.41 +0.5 3,387,081 + BWI76_RS16310 0.84 +0.5 3,387,098 + BWI76_RS16310 0.85 +0.0 3,387,098 + BWI76_RS16310 0.85 +0.0 3,387,143 + BWI76_RS16310 0.89 -0.2 3,387,445 + BWI76_RS16315 0.74 -1.1 3,387,455 + BWI76_RS16315 0.78 -0.4 3,388,085 - BWI76_RS16320 0.62 +0.4 3,388,101 - BWI76_RS16320 0.64 -1.0 3,388,101 - +0.7 3,388,136 + BWI76_RS16320 0.68 +0.3 3,388,187 - BWI76_RS16320 0.74 -0.9 3,388,187 - BWI76_RS16320 0.74 -0.0 3,388,187 - BWI76_RS16320 0.74 -0.2 3,388,219 + BWI76_RS16320 0.78 +0.3
Or see this region's nucleotide sequence