Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16130 and BWI76_RS16135 are separated by 9 nucleotides BWI76_RS16135 and BWI76_RS16140 are separated by 10 nucleotides BWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotides
BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740
_RS16130
BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952
_RS16135
BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619
_RS16140
BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316
_RS16145
Position (kb)
3347
3348
3349 Strain fitness (log2 ratio)
-1
0
1
2 at 3346.851 kb on + strand, within BWI76_RS16130 at 3347.026 kb on - strand, within BWI76_RS16130 at 3347.070 kb on - strand, within BWI76_RS16130 at 3347.259 kb on - strand, within BWI76_RS16130 at 3347.785 kb on - strand at 3347.918 kb on + strand, within BWI76_RS16135 at 3347.941 kb on + strand, within BWI76_RS16135 at 3347.949 kb on - strand, within BWI76_RS16135 at 3348.604 kb on - strand, within BWI76_RS16135 at 3349.004 kb on + strand at 3349.401 kb on - strand, within BWI76_RS16140 at 3349.411 kb on + strand, within BWI76_RS16140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,346,851 + BWI76_RS16130 0.34 -0.3 3,347,026 - BWI76_RS16130 0.47 -0.1 3,347,070 - BWI76_RS16130 0.50 -0.0 3,347,259 - BWI76_RS16130 0.64 +0.5 3,347,785 - -0.1 3,347,918 + BWI76_RS16135 0.14 +0.1 3,347,941 + BWI76_RS16135 0.16 -0.5 3,347,949 - BWI76_RS16135 0.17 +0.1 3,348,604 - BWI76_RS16135 0.71 +2.1 3,349,004 + -1.7 3,349,401 - BWI76_RS16140 0.67 -0.1 3,349,411 + BWI76_RS16140 0.68 +0.3
Or see this region's nucleotide sequence