Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16120

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16115 and BWI76_RS16120 are separated by 83 nucleotidesBWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotidesBWI76_RS16125 and BWI76_RS16130 overlap by 11 nucleotides BWI76_RS16115: BWI76_RS16115 - fumarate hydratase, at 3,343,519 to 3,345,174 _RS16115 BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641 _RS16120 BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398 _RS16125 BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740 _RS16130 Position (kb) 3345 3346Strain fitness (log2 ratio) -1 0 1at 3344.316 kb on - strand, within BWI76_RS16115at 3344.316 kb on - strand, within BWI76_RS16115at 3344.603 kb on + strand, within BWI76_RS16115at 3344.637 kb on + strand, within BWI76_RS16115at 3344.689 kb on - strand, within BWI76_RS16115at 3344.746 kb on - strand, within BWI76_RS16115at 3345.032 kb on + strandat 3345.032 kb on + strandat 3345.228 kb on + strandat 3345.392 kb on - strand, within BWI76_RS16120at 3345.440 kb on + strand, within BWI76_RS16120at 3345.585 kb on + strand, within BWI76_RS16120at 3345.593 kb on - strand, within BWI76_RS16120at 3345.654 kb on + strandat 3345.903 kb on + strand, within BWI76_RS16125at 3346.488 kb on - strandat 3346.494 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,344,316 - BWI76_RS16115 0.48 +0.5
3,344,316 - BWI76_RS16115 0.48 -1.1
3,344,603 + BWI76_RS16115 0.65 -1.2
3,344,637 + BWI76_RS16115 0.68 +0.8
3,344,689 - BWI76_RS16115 0.71 -0.0
3,344,746 - BWI76_RS16115 0.74 -0.3
3,345,032 + +0.1
3,345,032 + +0.0
3,345,228 + -0.8
3,345,392 - BWI76_RS16120 0.35 -0.3
3,345,440 + BWI76_RS16120 0.47 +0.7
3,345,585 + BWI76_RS16120 0.85 +0.4
3,345,593 - BWI76_RS16120 0.87 +0.4
3,345,654 + +0.7
3,345,903 + BWI76_RS16125 0.35 -0.2
3,346,488 - -1.1
3,346,494 + -0.3

Or see this region's nucleotide sequence