Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15180 and BWI76_RS15185 are separated by 111 nucleotides BWI76_RS15185 and BWI76_RS15190 are separated by 90 nucleotides BWI76_RS15190 and BWI76_RS15195 are separated by 107 nucleotides BWI76_RS15195 and BWI76_RS15200 overlap by 1 nucleotides
BWI76_RS15180: BWI76_RS15180 - altronate oxidoreductase, at 3,181,934 to 3,183,385
_RS15180
BWI76_RS15185: BWI76_RS15185 - N-acetyltransferase, at 3,183,497 to 3,184,027
_RS15185
BWI76_RS15190: BWI76_RS15190 - GGDEF domain-containing protein, at 3,184,118 to 3,185,530
_RS15190
BWI76_RS15195: BWI76_RS15195 - DUF4186 domain-containing protein, at 3,185,638 to 3,186,012
_RS15195
BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938
_RS15200
Position (kb)
3184
3185
3186 Strain fitness (log2 ratio)
-1
0
1 at 3183.141 kb on + strand at 3183.141 kb on + strand, within BWI76_RS15180 at 3183.345 kb on - strand at 3183.648 kb on + strand, within BWI76_RS15185 at 3183.910 kb on + strand, within BWI76_RS15185 at 3184.420 kb on + strand, within BWI76_RS15190 at 3184.503 kb on - strand, within BWI76_RS15190 at 3184.511 kb on + strand, within BWI76_RS15190 at 3184.607 kb on + strand, within BWI76_RS15190 at 3184.979 kb on - strand, within BWI76_RS15190 at 3185.286 kb on - strand, within BWI76_RS15190 at 3185.294 kb on - strand, within BWI76_RS15190 at 3185.446 kb on + strand at 3185.446 kb on + strand at 3185.543 kb on + strand at 3185.710 kb on - strand, within BWI76_RS15195 at 3185.881 kb on - strand, within BWI76_RS15195 at 3186.093 kb on - strand at 3186.326 kb on + strand, within BWI76_RS15200
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,183,141 + +0.7 3,183,141 + BWI76_RS15180 0.83 -0.1 3,183,345 - -0.4 3,183,648 + BWI76_RS15185 0.28 -1.1 3,183,910 + BWI76_RS15185 0.78 -0.2 3,184,420 + BWI76_RS15190 0.21 +0.1 3,184,503 - BWI76_RS15190 0.27 +0.5 3,184,511 + BWI76_RS15190 0.28 +0.8 3,184,607 + BWI76_RS15190 0.35 -0.1 3,184,979 - BWI76_RS15190 0.61 -0.7 3,185,286 - BWI76_RS15190 0.83 +0.3 3,185,294 - BWI76_RS15190 0.83 +0.9 3,185,446 + -0.7 3,185,446 + +0.0 3,185,543 + +0.2 3,185,710 - BWI76_RS15195 0.19 -0.4 3,185,881 - BWI76_RS15195 0.65 -0.0 3,186,093 - +0.6 3,186,326 + BWI76_RS15200 0.34 -0.0
Or see this region's nucleotide sequence