Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15020 and BWI76_RS15025 are separated by 29 nucleotides BWI76_RS15025 and BWI76_RS15030 overlap by 4 nucleotides BWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotides BWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotides
BWI76_RS15020: BWI76_RS15020 - 2-oxobutyrate oxidase, at 3,144,595 to 3,145,623
_RS15020
BWI76_RS15025: BWI76_RS15025 - NLPA lipoprotein, at 3,145,653 to 3,146,459
_RS15025
BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247
_RS15030
BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903
_RS15035
BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981
_RS15040
Position (kb) 3146
3147
3148 Strain fitness (log2 ratio) -1
0
1 at 3145.681 kb on + strand at 3145.689 kb on - strand at 3146.294 kb on - strand, within BWI76_RS15025 at 3146.888 kb on - strand, within BWI76_RS15030 at 3147.155 kb on - strand, within BWI76_RS15030 at 3147.407 kb on - strand, within BWI76_RS15035 at 3147.752 kb on + strand, within BWI76_RS15035 at 3148.203 kb on + strand, within BWI76_RS15040
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,145,681 + -0.5 3,145,689 - -0.5 3,146,294 - BWI76_RS15025 0.79 +0.8 3,146,888 - BWI76_RS15030 0.55 +0.4 3,147,155 - BWI76_RS15030 0.88 +0.0 3,147,407 - BWI76_RS15035 0.26 +0.6 3,147,752 + BWI76_RS15035 0.77 +1.7 3,148,203 + BWI76_RS15040 0.26 -0.7
Or see this region's nucleotide sequence