Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14980

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotidesBWI76_RS14975 and BWI76_RS14980 overlap by 4 nucleotidesBWI76_RS14980 and BWI76_RS14985 are separated by 4 nucleotidesBWI76_RS14985 and BWI76_RS14990 overlap by 11 nucleotides BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861 _RS14970 BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629 _RS14975 BWI76_RS14980: BWI76_RS14980 - hypothetical protein, at 3,139,626 to 3,140,156 _RS14980 BWI76_RS14985: BWI76_RS14985 - amino acid ABC transporter substrate-binding protein, at 3,140,161 to 3,141,003 _RS14985 BWI76_RS14990: BWI76_RS14990 - polar amino acid ABC transporter permease, at 3,140,993 to 3,141,745 _RS14990 Position (kb) 3139 3140 3141Strain fitness (log2 ratio) -1 0 1 2at 3139.204 kb on + strand, within BWI76_RS14975at 3139.430 kb on + strand, within BWI76_RS14975at 3139.477 kb on + strand, within BWI76_RS14975at 3139.643 kb on + strandat 3140.090 kb on + strand, within BWI76_RS14980at 3140.098 kb on - strand, within BWI76_RS14980at 3140.122 kb on + strandat 3140.174 kb on + strandat 3140.207 kb on + strandat 3140.210 kb on - strandat 3140.222 kb on - strandat 3140.285 kb on - strand, within BWI76_RS14985at 3140.558 kb on + strand, within BWI76_RS14985at 3140.593 kb on - strand, within BWI76_RS14985at 3140.667 kb on + strand, within BWI76_RS14985at 3140.703 kb on - strand, within BWI76_RS14985at 3140.714 kb on - strand, within BWI76_RS14985at 3140.735 kb on - strand, within BWI76_RS14985at 3140.736 kb on - strand, within BWI76_RS14985at 3140.828 kb on - strand, within BWI76_RS14985at 3140.894 kb on - strand, within BWI76_RS14985at 3141.068 kb on + strandat 3141.076 kb on + strand, within BWI76_RS14990at 3141.076 kb on + strand, within BWI76_RS14990at 3141.084 kb on - strand, within BWI76_RS14990at 3141.086 kb on + strand, within BWI76_RS14990at 3141.089 kb on - strand, within BWI76_RS14990at 3141.093 kb on + strand, within BWI76_RS14990at 3141.094 kb on - strand, within BWI76_RS14990at 3141.099 kb on - strand, within BWI76_RS14990at 3141.118 kb on + strand, within BWI76_RS14990at 3141.141 kb on - strand, within BWI76_RS14990at 3141.148 kb on - strand, within BWI76_RS14990

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,139,204 + BWI76_RS14975 0.44 -0.6
3,139,430 + BWI76_RS14975 0.74 -0.0
3,139,477 + BWI76_RS14975 0.80 +0.4
3,139,643 + -0.3
3,140,090 + BWI76_RS14980 0.87 +1.0
3,140,098 - BWI76_RS14980 0.89 -0.1
3,140,122 + +1.2
3,140,174 + -0.1
3,140,207 + +0.1
3,140,210 - -0.5
3,140,222 - -0.3
3,140,285 - BWI76_RS14985 0.15 +0.2
3,140,558 + BWI76_RS14985 0.47 -0.1
3,140,593 - BWI76_RS14985 0.51 -0.3
3,140,667 + BWI76_RS14985 0.60 +0.0
3,140,703 - BWI76_RS14985 0.64 -0.4
3,140,714 - BWI76_RS14985 0.66 +0.5
3,140,735 - BWI76_RS14985 0.68 +0.2
3,140,736 - BWI76_RS14985 0.68 +0.2
3,140,828 - BWI76_RS14985 0.79 -0.6
3,140,894 - BWI76_RS14985 0.87 +0.2
3,141,068 + -0.0
3,141,076 + BWI76_RS14990 0.11 -0.5
3,141,076 + BWI76_RS14990 0.11 -0.7
3,141,084 - BWI76_RS14990 0.12 -0.2
3,141,086 + BWI76_RS14990 0.12 +0.7
3,141,089 - BWI76_RS14990 0.13 -0.3
3,141,093 + BWI76_RS14990 0.13 -0.4
3,141,094 - BWI76_RS14990 0.13 +0.4
3,141,099 - BWI76_RS14990 0.14 -0.2
3,141,118 + BWI76_RS14990 0.17 +1.8
3,141,141 - BWI76_RS14990 0.20 -0.6
3,141,148 - BWI76_RS14990 0.21 -0.0

Or see this region's nucleotide sequence