Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotides BWI76_RS14975 and BWI76_RS14980 overlap by 4 nucleotides BWI76_RS14980 and BWI76_RS14985 are separated by 4 nucleotides BWI76_RS14985 and BWI76_RS14990 overlap by 11 nucleotides
BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861
_RS14970
BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629
_RS14975
BWI76_RS14980: BWI76_RS14980 - hypothetical protein, at 3,139,626 to 3,140,156
_RS14980
BWI76_RS14985: BWI76_RS14985 - amino acid ABC transporter substrate-binding protein, at 3,140,161 to 3,141,003
_RS14985
BWI76_RS14990: BWI76_RS14990 - polar amino acid ABC transporter permease, at 3,140,993 to 3,141,745
_RS14990
Position (kb)
3139
3140
3141 Strain fitness (log2 ratio)
-1
0
1
2 at 3139.204 kb on + strand, within BWI76_RS14975 at 3139.430 kb on + strand, within BWI76_RS14975 at 3139.477 kb on + strand, within BWI76_RS14975 at 3139.643 kb on + strand at 3140.090 kb on + strand, within BWI76_RS14980 at 3140.098 kb on - strand, within BWI76_RS14980 at 3140.122 kb on + strand at 3140.174 kb on + strand at 3140.207 kb on + strand at 3140.210 kb on - strand at 3140.222 kb on - strand at 3140.285 kb on - strand, within BWI76_RS14985 at 3140.558 kb on + strand, within BWI76_RS14985 at 3140.593 kb on - strand, within BWI76_RS14985 at 3140.667 kb on + strand, within BWI76_RS14985 at 3140.703 kb on - strand, within BWI76_RS14985 at 3140.714 kb on - strand, within BWI76_RS14985 at 3140.735 kb on - strand, within BWI76_RS14985 at 3140.736 kb on - strand, within BWI76_RS14985 at 3140.828 kb on - strand, within BWI76_RS14985 at 3140.894 kb on - strand, within BWI76_RS14985 at 3141.068 kb on + strand at 3141.076 kb on + strand, within BWI76_RS14990 at 3141.076 kb on + strand, within BWI76_RS14990 at 3141.084 kb on - strand, within BWI76_RS14990 at 3141.086 kb on + strand, within BWI76_RS14990 at 3141.089 kb on - strand, within BWI76_RS14990 at 3141.093 kb on + strand, within BWI76_RS14990 at 3141.094 kb on - strand, within BWI76_RS14990 at 3141.099 kb on - strand, within BWI76_RS14990 at 3141.118 kb on + strand, within BWI76_RS14990 at 3141.141 kb on - strand, within BWI76_RS14990 at 3141.148 kb on - strand, within BWI76_RS14990
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,139,204 + BWI76_RS14975 0.44 -0.6 3,139,430 + BWI76_RS14975 0.74 -0.0 3,139,477 + BWI76_RS14975 0.80 +0.4 3,139,643 + -0.3 3,140,090 + BWI76_RS14980 0.87 +1.0 3,140,098 - BWI76_RS14980 0.89 -0.1 3,140,122 + +1.2 3,140,174 + -0.1 3,140,207 + +0.1 3,140,210 - -0.5 3,140,222 - -0.3 3,140,285 - BWI76_RS14985 0.15 +0.2 3,140,558 + BWI76_RS14985 0.47 -0.1 3,140,593 - BWI76_RS14985 0.51 -0.3 3,140,667 + BWI76_RS14985 0.60 +0.0 3,140,703 - BWI76_RS14985 0.64 -0.4 3,140,714 - BWI76_RS14985 0.66 +0.5 3,140,735 - BWI76_RS14985 0.68 +0.2 3,140,736 - BWI76_RS14985 0.68 +0.2 3,140,828 - BWI76_RS14985 0.79 -0.6 3,140,894 - BWI76_RS14985 0.87 +0.2 3,141,068 + -0.0 3,141,076 + BWI76_RS14990 0.11 -0.5 3,141,076 + BWI76_RS14990 0.11 -0.7 3,141,084 - BWI76_RS14990 0.12 -0.2 3,141,086 + BWI76_RS14990 0.12 +0.7 3,141,089 - BWI76_RS14990 0.13 -0.3 3,141,093 + BWI76_RS14990 0.13 -0.4 3,141,094 - BWI76_RS14990 0.13 +0.4 3,141,099 - BWI76_RS14990 0.14 -0.2 3,141,118 + BWI76_RS14990 0.17 +1.8 3,141,141 - BWI76_RS14990 0.20 -0.6 3,141,148 - BWI76_RS14990 0.21 -0.0
Or see this region's nucleotide sequence