Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14820

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14810 and BWI76_RS14815 are separated by 21 nucleotidesBWI76_RS14815 and BWI76_RS14820 are separated by 98 nucleotidesBWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotidesBWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides BWI76_RS14810: BWI76_RS14810 - carboxymuconolactone decarboxylase, at 3,104,097 to 3,104,897 _RS14810 BWI76_RS14815: BWI76_RS14815 - MdaB protein, at 3,104,919 to 3,105,515 _RS14815 BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522 _RS14820 BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332 _RS14825 BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904 _RS14830 Position (kb) 3105 3106 3107Strain fitness (log2 ratio) -1 0 1 2at 3104.648 kb on + strand, within BWI76_RS14810at 3104.656 kb on - strand, within BWI76_RS14810at 3104.702 kb on - strand, within BWI76_RS14810at 3104.726 kb on - strand, within BWI76_RS14810at 3104.736 kb on - strand, within BWI76_RS14810at 3104.790 kb on + strand, within BWI76_RS14810at 3104.790 kb on + strand, within BWI76_RS14810at 3105.041 kb on + strand, within BWI76_RS14815at 3105.059 kb on + strand, within BWI76_RS14815at 3105.067 kb on - strand, within BWI76_RS14815at 3105.067 kb on - strand, within BWI76_RS14815at 3105.393 kb on - strand, within BWI76_RS14815at 3105.432 kb on + strand, within BWI76_RS14815at 3105.475 kb on + strandat 3105.676 kb on + strandat 3106.369 kb on + strand, within BWI76_RS14820at 3106.660 kb on - strandat 3106.674 kb on - strandat 3106.846 kb on - strand, within BWI76_RS14825at 3106.983 kb on + strand, within BWI76_RS14825

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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3,104,648 + BWI76_RS14810 0.69 -0.2
3,104,656 - BWI76_RS14810 0.70 -0.8
3,104,702 - BWI76_RS14810 0.76 +0.1
3,104,726 - BWI76_RS14810 0.79 -1.0
3,104,736 - BWI76_RS14810 0.80 -0.1
3,104,790 + BWI76_RS14810 0.87 -0.8
3,104,790 + BWI76_RS14810 0.87 -0.6
3,105,041 + BWI76_RS14815 0.20 +1.0
3,105,059 + BWI76_RS14815 0.23 -0.3
3,105,067 - BWI76_RS14815 0.25 -0.5
3,105,067 - BWI76_RS14815 0.25 -0.2
3,105,393 - BWI76_RS14815 0.79 -0.2
3,105,432 + BWI76_RS14815 0.86 -0.2
3,105,475 + +0.0
3,105,676 + -0.1
3,106,369 + BWI76_RS14820 0.83 -0.9
3,106,660 - +0.9
3,106,674 - +0.0
3,106,846 - BWI76_RS14825 0.19 -0.7
3,106,983 + BWI76_RS14825 0.42 +2.2

Or see this region's nucleotide sequence