Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14810 and BWI76_RS14815 are separated by 21 nucleotides BWI76_RS14815 and BWI76_RS14820 are separated by 98 nucleotides BWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotides BWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides
BWI76_RS14810: BWI76_RS14810 - carboxymuconolactone decarboxylase, at 3,104,097 to 3,104,897
_RS14810
BWI76_RS14815: BWI76_RS14815 - MdaB protein, at 3,104,919 to 3,105,515
_RS14815
BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522
_RS14820
BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332
_RS14825
BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904
_RS14830
Position (kb)
3105
3106
3107 Strain fitness (log2 ratio)
-1
0
1
2 at 3104.648 kb on + strand, within BWI76_RS14810 at 3104.656 kb on - strand, within BWI76_RS14810 at 3104.702 kb on - strand, within BWI76_RS14810 at 3104.726 kb on - strand, within BWI76_RS14810 at 3104.736 kb on - strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3105.041 kb on + strand, within BWI76_RS14815 at 3105.059 kb on + strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.393 kb on - strand, within BWI76_RS14815 at 3105.432 kb on + strand, within BWI76_RS14815 at 3105.475 kb on + strand at 3105.676 kb on + strand at 3106.369 kb on + strand, within BWI76_RS14820 at 3106.660 kb on - strand at 3106.674 kb on - strand at 3106.846 kb on - strand, within BWI76_RS14825 at 3106.983 kb on + strand, within BWI76_RS14825
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 3,104,648 + BWI76_RS14810 0.69 -0.2 3,104,656 - BWI76_RS14810 0.70 -0.8 3,104,702 - BWI76_RS14810 0.76 +0.1 3,104,726 - BWI76_RS14810 0.79 -1.0 3,104,736 - BWI76_RS14810 0.80 -0.1 3,104,790 + BWI76_RS14810 0.87 -0.8 3,104,790 + BWI76_RS14810 0.87 -0.6 3,105,041 + BWI76_RS14815 0.20 +1.0 3,105,059 + BWI76_RS14815 0.23 -0.3 3,105,067 - BWI76_RS14815 0.25 -0.5 3,105,067 - BWI76_RS14815 0.25 -0.2 3,105,393 - BWI76_RS14815 0.79 -0.2 3,105,432 + BWI76_RS14815 0.86 -0.2 3,105,475 + +0.0 3,105,676 + -0.1 3,106,369 + BWI76_RS14820 0.83 -0.9 3,106,660 - +0.9 3,106,674 - +0.0 3,106,846 - BWI76_RS14825 0.19 -0.7 3,106,983 + BWI76_RS14825 0.42 +2.2
Or see this region's nucleotide sequence