Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14265

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotidesBWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotidesBWI76_RS14265 and BWI76_RS14270 are separated by 365 nucleotides BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778 _RS14255 BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930 _RS14260 BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027 _RS14265 BWI76_RS14270: BWI76_RS14270 - transporter, at 2,984,393 to 2,985,205 _RS14270 Position (kb) 2983 2984 2985Strain fitness (log2 ratio) -2 -1 0 1 2at 2982.162 kb on + strand, within BWI76_RS14255at 2982.170 kb on - strand, within BWI76_RS14255at 2982.227 kb on - strand, within BWI76_RS14255at 2982.232 kb on - strand, within BWI76_RS14255at 2982.253 kb on + strand, within BWI76_RS14255at 2982.261 kb on - strand, within BWI76_RS14255at 2982.271 kb on + strand, within BWI76_RS14255at 2982.297 kb on + strand, within BWI76_RS14255at 2982.366 kb on - strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255at 2982.854 kb on - strand, within BWI76_RS14260at 2982.957 kb on + strandat 2982.974 kb on - strandat 2983.006 kb on - strandat 2983.024 kb on + strandat 2983.032 kb on + strandat 2983.039 kb on - strandat 2983.039 kb on - strandat 2983.055 kb on - strandat 2983.171 kb on + strandat 2983.225 kb on + strand, within BWI76_RS14265at 2983.372 kb on - strand, within BWI76_RS14265at 2983.406 kb on - strand, within BWI76_RS14265at 2983.534 kb on + strand, within BWI76_RS14265at 2983.636 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.757 kb on + strand, within BWI76_RS14265at 2983.765 kb on - strand, within BWI76_RS14265at 2983.819 kb on + strand, within BWI76_RS14265at 2983.862 kb on - strand, within BWI76_RS14265at 2983.862 kb on - strand, within BWI76_RS14265at 2983.872 kb on - strand, within BWI76_RS14265at 2983.993 kb on - strandat 2984.010 kb on + strandat 2984.020 kb on + strandat 2984.022 kb on + strandat 2984.029 kb on - strandat 2984.478 kb on - strand, within BWI76_RS14270at 2984.538 kb on - strand, within BWI76_RS14270at 2984.642 kb on - strand, within BWI76_RS14270at 2984.673 kb on + strand, within BWI76_RS14270at 2984.673 kb on + strand, within BWI76_RS14270at 2984.775 kb on + strand, within BWI76_RS14270at 2984.835 kb on - strandat 2984.938 kb on + strand, within BWI76_RS14270at 2985.009 kb on + strand, within BWI76_RS14270

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,982,162 + BWI76_RS14255 0.39 +0.1
2,982,170 - BWI76_RS14255 0.39 -0.8
2,982,227 - BWI76_RS14255 0.45 +0.4
2,982,232 - BWI76_RS14255 0.46 -0.1
2,982,253 + BWI76_RS14255 0.48 -0.2
2,982,261 - BWI76_RS14255 0.48 +0.0
2,982,271 + BWI76_RS14255 0.49 -0.6
2,982,297 + BWI76_RS14255 0.52 -0.5
2,982,366 - BWI76_RS14255 0.59 +0.1
2,982,488 + BWI76_RS14255 0.71 +0.5
2,982,488 + BWI76_RS14255 0.71 -0.7
2,982,854 - BWI76_RS14260 0.51 -0.4
2,982,957 + -1.1
2,982,974 - -0.2
2,983,006 - -1.1
2,983,024 + -0.1
2,983,032 + -0.2
2,983,039 - -0.1
2,983,039 - +0.2
2,983,055 - -0.4
2,983,171 + -0.5
2,983,225 + BWI76_RS14265 0.14 +0.1
2,983,372 - BWI76_RS14265 0.30 -0.2
2,983,406 - BWI76_RS14265 0.34 +0.8
2,983,534 + BWI76_RS14265 0.47 -0.9
2,983,636 + BWI76_RS14265 0.58 +0.9
2,983,757 + BWI76_RS14265 0.71 +2.4
2,983,757 + BWI76_RS14265 0.71 +0.7
2,983,757 + BWI76_RS14265 0.71 +1.5
2,983,765 - BWI76_RS14265 0.72 +0.6
2,983,819 + BWI76_RS14265 0.78 +0.9
2,983,862 - BWI76_RS14265 0.82 +0.5
2,983,862 - BWI76_RS14265 0.82 -0.5
2,983,872 - BWI76_RS14265 0.83 -0.1
2,983,993 - -0.5
2,984,010 + +0.9
2,984,020 + -0.1
2,984,022 + -1.5
2,984,029 - -0.6
2,984,478 - BWI76_RS14270 0.10 +0.6
2,984,538 - BWI76_RS14270 0.18 -2.0
2,984,642 - BWI76_RS14270 0.31 +0.1
2,984,673 + BWI76_RS14270 0.34 -0.1
2,984,673 + BWI76_RS14270 0.34 +0.4
2,984,775 + BWI76_RS14270 0.47 -0.1
2,984,835 - +2.3
2,984,938 + BWI76_RS14270 0.67 -1.2
2,985,009 + BWI76_RS14270 0.76 -0.6

Or see this region's nucleotide sequence