Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotides BWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotides BWI76_RS14265 and BWI76_RS14270 are separated by 365 nucleotides
BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778
_RS14255
BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930
_RS14260
BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027
_RS14265
BWI76_RS14270: BWI76_RS14270 - transporter, at 2,984,393 to 2,985,205
_RS14270
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2982.162 kb on + strand, within BWI76_RS14255 at 2982.170 kb on - strand, within BWI76_RS14255 at 2982.227 kb on - strand, within BWI76_RS14255 at 2982.232 kb on - strand, within BWI76_RS14255 at 2982.253 kb on + strand, within BWI76_RS14255 at 2982.261 kb on - strand, within BWI76_RS14255 at 2982.271 kb on + strand, within BWI76_RS14255 at 2982.297 kb on + strand, within BWI76_RS14255 at 2982.366 kb on - strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.854 kb on - strand, within BWI76_RS14260 at 2982.957 kb on + strand at 2982.974 kb on - strand at 2983.006 kb on - strand at 2983.024 kb on + strand at 2983.032 kb on + strand at 2983.039 kb on - strand at 2983.039 kb on - strand at 2983.055 kb on - strand at 2983.171 kb on + strand at 2983.225 kb on + strand, within BWI76_RS14265 at 2983.372 kb on - strand, within BWI76_RS14265 at 2983.406 kb on - strand, within BWI76_RS14265 at 2983.534 kb on + strand, within BWI76_RS14265 at 2983.636 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.765 kb on - strand, within BWI76_RS14265 at 2983.819 kb on + strand, within BWI76_RS14265 at 2983.862 kb on - strand, within BWI76_RS14265 at 2983.862 kb on - strand, within BWI76_RS14265 at 2983.872 kb on - strand, within BWI76_RS14265 at 2983.993 kb on - strand at 2984.010 kb on + strand at 2984.020 kb on + strand at 2984.022 kb on + strand at 2984.029 kb on - strand at 2984.478 kb on - strand, within BWI76_RS14270 at 2984.538 kb on - strand, within BWI76_RS14270 at 2984.642 kb on - strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.775 kb on + strand, within BWI76_RS14270 at 2984.835 kb on - strand at 2984.938 kb on + strand, within BWI76_RS14270 at 2985.009 kb on + strand, within BWI76_RS14270
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,982,162 + BWI76_RS14255 0.39 +0.1 2,982,170 - BWI76_RS14255 0.39 -0.8 2,982,227 - BWI76_RS14255 0.45 +0.4 2,982,232 - BWI76_RS14255 0.46 -0.1 2,982,253 + BWI76_RS14255 0.48 -0.2 2,982,261 - BWI76_RS14255 0.48 +0.0 2,982,271 + BWI76_RS14255 0.49 -0.6 2,982,297 + BWI76_RS14255 0.52 -0.5 2,982,366 - BWI76_RS14255 0.59 +0.1 2,982,488 + BWI76_RS14255 0.71 +0.5 2,982,488 + BWI76_RS14255 0.71 -0.7 2,982,854 - BWI76_RS14260 0.51 -0.4 2,982,957 + -1.1 2,982,974 - -0.2 2,983,006 - -1.1 2,983,024 + -0.1 2,983,032 + -0.2 2,983,039 - -0.1 2,983,039 - +0.2 2,983,055 - -0.4 2,983,171 + -0.5 2,983,225 + BWI76_RS14265 0.14 +0.1 2,983,372 - BWI76_RS14265 0.30 -0.2 2,983,406 - BWI76_RS14265 0.34 +0.8 2,983,534 + BWI76_RS14265 0.47 -0.9 2,983,636 + BWI76_RS14265 0.58 +0.9 2,983,757 + BWI76_RS14265 0.71 +2.4 2,983,757 + BWI76_RS14265 0.71 +0.7 2,983,757 + BWI76_RS14265 0.71 +1.5 2,983,765 - BWI76_RS14265 0.72 +0.6 2,983,819 + BWI76_RS14265 0.78 +0.9 2,983,862 - BWI76_RS14265 0.82 +0.5 2,983,862 - BWI76_RS14265 0.82 -0.5 2,983,872 - BWI76_RS14265 0.83 -0.1 2,983,993 - -0.5 2,984,010 + +0.9 2,984,020 + -0.1 2,984,022 + -1.5 2,984,029 - -0.6 2,984,478 - BWI76_RS14270 0.10 +0.6 2,984,538 - BWI76_RS14270 0.18 -2.0 2,984,642 - BWI76_RS14270 0.31 +0.1 2,984,673 + BWI76_RS14270 0.34 -0.1 2,984,673 + BWI76_RS14270 0.34 +0.4 2,984,775 + BWI76_RS14270 0.47 -0.1 2,984,835 - +2.3 2,984,938 + BWI76_RS14270 0.67 -1.2 2,985,009 + BWI76_RS14270 0.76 -0.6
Or see this region's nucleotide sequence