Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13725 and BWI76_RS13730 overlap by 59 nucleotides BWI76_RS13730 and BWI76_RS13735 are separated by 102 nucleotides BWI76_RS13735 and BWI76_RS13740 are separated by 48 nucleotides BWI76_RS13740 and BWI76_RS13745 are separated by 11 nucleotides BWI76_RS13745 and BWI76_RS13750 overlap by 8 nucleotides
BWI76_RS13725: BWI76_RS13725 - aromatic amino acid exporter, at 2,863,232 to 2,864,110
_RS13725
BWI76_RS13730: BWI76_RS13730 - hypothetical protein, at 2,864,052 to 2,864,235
_RS13730
BWI76_RS13735: BWI76_RS13735 - sulfate ABC transporter substrate-binding protein, at 2,864,338 to 2,864,925
_RS13735
BWI76_RS13740: BWI76_RS13740 - formate dehydrogenase-N subunit alpha, at 2,864,974 to 2,867,385
_RS13740
BWI76_RS13745: BWI76_RS13745 - formate dehydrogenase subunit beta, at 2,867,397 to 2,868,281
_RS13745
BWI76_RS13750: BWI76_RS13750 - formate dehydrogenase, at 2,868,274 to 2,868,930
_RS13750
Position (kb)
2864
2865
2866
2867
2868 Strain fitness (log2 ratio)
-2
-1
0
1 at 2863.991 kb on + strand, within BWI76_RS13725 at 2863.994 kb on - strand, within BWI76_RS13725 at 2864.012 kb on - strand, within BWI76_RS13725 at 2864.020 kb on + strand, within BWI76_RS13725 at 2864.034 kb on + strand at 2864.063 kb on + strand at 2864.063 kb on + strand at 2864.065 kb on + strand at 2864.065 kb on + strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.073 kb on - strand at 2864.087 kb on - strand at 2864.190 kb on + strand, within BWI76_RS13730 at 2864.198 kb on - strand, within BWI76_RS13730 at 2864.323 kb on + strand at 2864.328 kb on + strand at 2864.336 kb on + strand at 2864.336 kb on - strand at 2864.393 kb on - strand at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.441 kb on - strand, within BWI76_RS13735 at 2864.481 kb on + strand, within BWI76_RS13735 at 2864.489 kb on - strand, within BWI76_RS13735 at 2864.938 kb on - strand at 2865.246 kb on + strand, within BWI76_RS13740 at 2865.514 kb on + strand, within BWI76_RS13740 at 2865.543 kb on + strand, within BWI76_RS13740 at 2866.005 kb on - strand, within BWI76_RS13740 at 2866.273 kb on + strand, within BWI76_RS13740 at 2866.502 kb on + strand, within BWI76_RS13740 at 2866.659 kb on - strand, within BWI76_RS13740 at 2866.671 kb on + strand, within BWI76_RS13740 at 2866.773 kb on + strand, within BWI76_RS13740 at 2866.799 kb on + strand, within BWI76_RS13740 at 2867.054 kb on + strand, within BWI76_RS13740 at 2867.220 kb on + strand at 2867.235 kb on + strand at 2867.298 kb on - strand at 2867.372 kb on - strand at 2867.449 kb on + strand at 2867.537 kb on + strand, within BWI76_RS13745 at 2867.545 kb on - strand, within BWI76_RS13745 at 2867.589 kb on - strand, within BWI76_RS13745 at 2868.041 kb on + strand, within BWI76_RS13745 at 2868.076 kb on + strand, within BWI76_RS13745 at 2868.094 kb on + strand, within BWI76_RS13745 at 2868.114 kb on - strand, within BWI76_RS13745 at 2868.358 kb on - strand, within BWI76_RS13750
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,863,991 + BWI76_RS13725 0.86 +0.4 2,863,994 - BWI76_RS13725 0.87 +0.3 2,864,012 - BWI76_RS13725 0.89 +0.4 2,864,020 + BWI76_RS13725 0.90 +0.1 2,864,034 + -0.2 2,864,063 + -0.6 2,864,063 + -0.4 2,864,065 + -0.4 2,864,065 + -0.0 2,864,071 - +0.2 2,864,071 - +0.1 2,864,071 - -0.6 2,864,071 - -0.1 2,864,073 - +0.1 2,864,087 - -0.2 2,864,190 + BWI76_RS13730 0.75 -0.0 2,864,198 - BWI76_RS13730 0.79 +0.4 2,864,323 + +0.4 2,864,328 + -0.3 2,864,336 + -0.6 2,864,336 - -0.9 2,864,393 - -0.1 2,864,404 + BWI76_RS13735 0.11 -0.1 2,864,404 + BWI76_RS13735 0.11 -0.4 2,864,404 + BWI76_RS13735 0.11 +0.2 2,864,404 + BWI76_RS13735 0.11 -0.0 2,864,412 - BWI76_RS13735 0.13 -0.9 2,864,412 - BWI76_RS13735 0.13 +1.3 2,864,412 - BWI76_RS13735 0.13 -0.4 2,864,441 - BWI76_RS13735 0.18 -1.1 2,864,481 + BWI76_RS13735 0.24 +0.0 2,864,489 - BWI76_RS13735 0.26 +0.0 2,864,938 - -0.4 2,865,246 + BWI76_RS13740 0.11 +1.1 2,865,514 + BWI76_RS13740 0.22 +0.7 2,865,543 + BWI76_RS13740 0.24 -0.1 2,866,005 - BWI76_RS13740 0.43 +0.2 2,866,273 + BWI76_RS13740 0.54 -0.5 2,866,502 + BWI76_RS13740 0.63 +0.9 2,866,659 - BWI76_RS13740 0.70 -0.3 2,866,671 + BWI76_RS13740 0.70 -0.1 2,866,773 + BWI76_RS13740 0.75 -0.2 2,866,799 + BWI76_RS13740 0.76 +0.7 2,867,054 + BWI76_RS13740 0.86 -0.1 2,867,220 + +0.0 2,867,235 + +1.0 2,867,298 - -2.5 2,867,372 - -0.6 2,867,449 + -0.3 2,867,537 + BWI76_RS13745 0.16 -0.5 2,867,545 - BWI76_RS13745 0.17 -0.4 2,867,589 - BWI76_RS13745 0.22 -0.1 2,868,041 + BWI76_RS13745 0.73 +0.4 2,868,076 + BWI76_RS13745 0.77 -0.0 2,868,094 + BWI76_RS13745 0.79 -0.1 2,868,114 - BWI76_RS13745 0.81 -0.8 2,868,358 - BWI76_RS13750 0.13 -0.7
Or see this region's nucleotide sequence