Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13740

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13725 and BWI76_RS13730 overlap by 59 nucleotidesBWI76_RS13730 and BWI76_RS13735 are separated by 102 nucleotidesBWI76_RS13735 and BWI76_RS13740 are separated by 48 nucleotidesBWI76_RS13740 and BWI76_RS13745 are separated by 11 nucleotidesBWI76_RS13745 and BWI76_RS13750 overlap by 8 nucleotides BWI76_RS13725: BWI76_RS13725 - aromatic amino acid exporter, at 2,863,232 to 2,864,110 _RS13725 BWI76_RS13730: BWI76_RS13730 - hypothetical protein, at 2,864,052 to 2,864,235 _RS13730 BWI76_RS13735: BWI76_RS13735 - sulfate ABC transporter substrate-binding protein, at 2,864,338 to 2,864,925 _RS13735 BWI76_RS13740: BWI76_RS13740 - formate dehydrogenase-N subunit alpha, at 2,864,974 to 2,867,385 _RS13740 BWI76_RS13745: BWI76_RS13745 - formate dehydrogenase subunit beta, at 2,867,397 to 2,868,281 _RS13745 BWI76_RS13750: BWI76_RS13750 - formate dehydrogenase, at 2,868,274 to 2,868,930 _RS13750 Position (kb) 2864 2865 2866 2867 2868Strain fitness (log2 ratio) -2 -1 0 1at 2863.991 kb on + strand, within BWI76_RS13725at 2863.994 kb on - strand, within BWI76_RS13725at 2864.012 kb on - strand, within BWI76_RS13725at 2864.020 kb on + strand, within BWI76_RS13725at 2864.034 kb on + strandat 2864.063 kb on + strandat 2864.063 kb on + strandat 2864.065 kb on + strandat 2864.065 kb on + strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.073 kb on - strandat 2864.087 kb on - strandat 2864.190 kb on + strand, within BWI76_RS13730at 2864.198 kb on - strand, within BWI76_RS13730at 2864.323 kb on + strandat 2864.328 kb on + strandat 2864.336 kb on + strandat 2864.336 kb on - strandat 2864.393 kb on - strandat 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.441 kb on - strand, within BWI76_RS13735at 2864.481 kb on + strand, within BWI76_RS13735at 2864.489 kb on - strand, within BWI76_RS13735at 2864.938 kb on - strandat 2865.246 kb on + strand, within BWI76_RS13740at 2865.514 kb on + strand, within BWI76_RS13740at 2865.543 kb on + strand, within BWI76_RS13740at 2866.005 kb on - strand, within BWI76_RS13740at 2866.273 kb on + strand, within BWI76_RS13740at 2866.502 kb on + strand, within BWI76_RS13740at 2866.659 kb on - strand, within BWI76_RS13740at 2866.671 kb on + strand, within BWI76_RS13740at 2866.773 kb on + strand, within BWI76_RS13740at 2866.799 kb on + strand, within BWI76_RS13740at 2867.054 kb on + strand, within BWI76_RS13740at 2867.220 kb on + strandat 2867.235 kb on + strandat 2867.298 kb on - strandat 2867.372 kb on - strandat 2867.449 kb on + strandat 2867.537 kb on + strand, within BWI76_RS13745at 2867.545 kb on - strand, within BWI76_RS13745at 2867.589 kb on - strand, within BWI76_RS13745at 2868.041 kb on + strand, within BWI76_RS13745at 2868.076 kb on + strand, within BWI76_RS13745at 2868.094 kb on + strand, within BWI76_RS13745at 2868.114 kb on - strand, within BWI76_RS13745at 2868.358 kb on - strand, within BWI76_RS13750

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,863,991 + BWI76_RS13725 0.86 +0.4
2,863,994 - BWI76_RS13725 0.87 +0.3
2,864,012 - BWI76_RS13725 0.89 +0.4
2,864,020 + BWI76_RS13725 0.90 +0.1
2,864,034 + -0.2
2,864,063 + -0.6
2,864,063 + -0.4
2,864,065 + -0.4
2,864,065 + -0.0
2,864,071 - +0.2
2,864,071 - +0.1
2,864,071 - -0.6
2,864,071 - -0.1
2,864,073 - +0.1
2,864,087 - -0.2
2,864,190 + BWI76_RS13730 0.75 -0.0
2,864,198 - BWI76_RS13730 0.79 +0.4
2,864,323 + +0.4
2,864,328 + -0.3
2,864,336 + -0.6
2,864,336 - -0.9
2,864,393 - -0.1
2,864,404 + BWI76_RS13735 0.11 -0.1
2,864,404 + BWI76_RS13735 0.11 -0.4
2,864,404 + BWI76_RS13735 0.11 +0.2
2,864,404 + BWI76_RS13735 0.11 -0.0
2,864,412 - BWI76_RS13735 0.13 -0.9
2,864,412 - BWI76_RS13735 0.13 +1.3
2,864,412 - BWI76_RS13735 0.13 -0.4
2,864,441 - BWI76_RS13735 0.18 -1.1
2,864,481 + BWI76_RS13735 0.24 +0.0
2,864,489 - BWI76_RS13735 0.26 +0.0
2,864,938 - -0.4
2,865,246 + BWI76_RS13740 0.11 +1.1
2,865,514 + BWI76_RS13740 0.22 +0.7
2,865,543 + BWI76_RS13740 0.24 -0.1
2,866,005 - BWI76_RS13740 0.43 +0.2
2,866,273 + BWI76_RS13740 0.54 -0.5
2,866,502 + BWI76_RS13740 0.63 +0.9
2,866,659 - BWI76_RS13740 0.70 -0.3
2,866,671 + BWI76_RS13740 0.70 -0.1
2,866,773 + BWI76_RS13740 0.75 -0.2
2,866,799 + BWI76_RS13740 0.76 +0.7
2,867,054 + BWI76_RS13740 0.86 -0.1
2,867,220 + +0.0
2,867,235 + +1.0
2,867,298 - -2.5
2,867,372 - -0.6
2,867,449 + -0.3
2,867,537 + BWI76_RS13745 0.16 -0.5
2,867,545 - BWI76_RS13745 0.17 -0.4
2,867,589 - BWI76_RS13745 0.22 -0.1
2,868,041 + BWI76_RS13745 0.73 +0.4
2,868,076 + BWI76_RS13745 0.77 -0.0
2,868,094 + BWI76_RS13745 0.79 -0.1
2,868,114 - BWI76_RS13745 0.81 -0.8
2,868,358 - BWI76_RS13750 0.13 -0.7

Or see this region's nucleotide sequence