Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13730

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13720 and BWI76_RS13725 overlap by 4 nucleotidesBWI76_RS13725 and BWI76_RS13730 overlap by 59 nucleotidesBWI76_RS13730 and BWI76_RS13735 are separated by 102 nucleotidesBWI76_RS13735 and BWI76_RS13740 are separated by 48 nucleotides BWI76_RS13720: BWI76_RS13720 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 2,862,462 to 2,863,235 _RS13720 BWI76_RS13725: BWI76_RS13725 - aromatic amino acid exporter, at 2,863,232 to 2,864,110 _RS13725 BWI76_RS13730: BWI76_RS13730 - hypothetical protein, at 2,864,052 to 2,864,235 _RS13730 BWI76_RS13735: BWI76_RS13735 - sulfate ABC transporter substrate-binding protein, at 2,864,338 to 2,864,925 _RS13735 BWI76_RS13740: BWI76_RS13740 - formate dehydrogenase-N subunit alpha, at 2,864,974 to 2,867,385 _RS13740 Position (kb) 2864 2865Strain fitness (log2 ratio) -1 0 1at 2863.137 kb on - strand, within BWI76_RS13720at 2863.254 kb on - strandat 2863.258 kb on + strandat 2863.322 kb on - strand, within BWI76_RS13725at 2863.322 kb on - strand, within BWI76_RS13725at 2863.424 kb on + strand, within BWI76_RS13725at 2863.437 kb on + strand, within BWI76_RS13725at 2863.475 kb on + strand, within BWI76_RS13725at 2863.475 kb on + strand, within BWI76_RS13725at 2863.483 kb on - strand, within BWI76_RS13725at 2863.483 kb on - strand, within BWI76_RS13725at 2863.483 kb on - strand, within BWI76_RS13725at 2863.522 kb on + strand, within BWI76_RS13725at 2863.523 kb on + strand, within BWI76_RS13725at 2863.530 kb on - strandat 2863.535 kb on + strand, within BWI76_RS13725at 2863.549 kb on + strand, within BWI76_RS13725at 2863.590 kb on + strand, within BWI76_RS13725at 2863.677 kb on + strand, within BWI76_RS13725at 2863.702 kb on + strand, within BWI76_RS13725at 2863.797 kb on + strand, within BWI76_RS13725at 2863.826 kb on + strand, within BWI76_RS13725at 2863.830 kb on + strand, within BWI76_RS13725at 2863.836 kb on - strand, within BWI76_RS13725at 2863.840 kb on + strand, within BWI76_RS13725at 2863.840 kb on + strand, within BWI76_RS13725at 2863.852 kb on + strand, within BWI76_RS13725at 2863.855 kb on - strand, within BWI76_RS13725at 2863.883 kb on - strand, within BWI76_RS13725at 2863.959 kb on + strand, within BWI76_RS13725at 2863.991 kb on + strand, within BWI76_RS13725at 2863.994 kb on - strand, within BWI76_RS13725at 2864.012 kb on - strand, within BWI76_RS13725at 2864.020 kb on + strand, within BWI76_RS13725at 2864.034 kb on + strandat 2864.063 kb on + strandat 2864.063 kb on + strandat 2864.065 kb on + strandat 2864.065 kb on + strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.071 kb on - strandat 2864.073 kb on - strandat 2864.087 kb on - strandat 2864.190 kb on + strand, within BWI76_RS13730at 2864.198 kb on - strand, within BWI76_RS13730at 2864.323 kb on + strandat 2864.328 kb on + strandat 2864.336 kb on + strandat 2864.336 kb on - strandat 2864.393 kb on - strandat 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.404 kb on + strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.412 kb on - strand, within BWI76_RS13735at 2864.441 kb on - strand, within BWI76_RS13735at 2864.481 kb on + strand, within BWI76_RS13735at 2864.489 kb on - strand, within BWI76_RS13735at 2864.938 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,863,137 - BWI76_RS13720 0.87 -1.4
2,863,254 - -0.5
2,863,258 + -0.0
2,863,322 - BWI76_RS13725 0.10 -0.2
2,863,322 - BWI76_RS13725 0.10 -0.2
2,863,424 + BWI76_RS13725 0.22 -0.8
2,863,437 + BWI76_RS13725 0.23 -0.5
2,863,475 + BWI76_RS13725 0.28 -0.0
2,863,475 + BWI76_RS13725 0.28 +0.6
2,863,483 - BWI76_RS13725 0.29 -0.3
2,863,483 - BWI76_RS13725 0.29 -0.9
2,863,483 - BWI76_RS13725 0.29 -0.1
2,863,522 + BWI76_RS13725 0.33 -0.4
2,863,523 + BWI76_RS13725 0.33 -0.8
2,863,530 - -0.4
2,863,535 + BWI76_RS13725 0.34 -0.1
2,863,549 + BWI76_RS13725 0.36 -0.4
2,863,590 + BWI76_RS13725 0.41 -0.2
2,863,677 + BWI76_RS13725 0.51 -1.2
2,863,702 + BWI76_RS13725 0.53 -0.7
2,863,797 + BWI76_RS13725 0.64 -1.0
2,863,826 + BWI76_RS13725 0.68 -0.4
2,863,830 + BWI76_RS13725 0.68 -0.0
2,863,836 - BWI76_RS13725 0.69 -0.1
2,863,840 + BWI76_RS13725 0.69 +0.3
2,863,840 + BWI76_RS13725 0.69 -0.2
2,863,852 + BWI76_RS13725 0.71 -0.6
2,863,855 - BWI76_RS13725 0.71 -0.5
2,863,883 - BWI76_RS13725 0.74 +0.4
2,863,959 + BWI76_RS13725 0.83 -0.1
2,863,991 + BWI76_RS13725 0.86 +0.4
2,863,994 - BWI76_RS13725 0.87 +0.3
2,864,012 - BWI76_RS13725 0.89 +0.4
2,864,020 + BWI76_RS13725 0.90 +0.1
2,864,034 + -0.2
2,864,063 + -0.6
2,864,063 + -0.4
2,864,065 + -0.4
2,864,065 + -0.0
2,864,071 - +0.2
2,864,071 - +0.1
2,864,071 - -0.6
2,864,071 - -0.1
2,864,073 - +0.1
2,864,087 - -0.2
2,864,190 + BWI76_RS13730 0.75 -0.0
2,864,198 - BWI76_RS13730 0.79 +0.4
2,864,323 + +0.4
2,864,328 + -0.3
2,864,336 + -0.6
2,864,336 - -0.9
2,864,393 - -0.1
2,864,404 + BWI76_RS13735 0.11 -0.1
2,864,404 + BWI76_RS13735 0.11 -0.4
2,864,404 + BWI76_RS13735 0.11 +0.2
2,864,404 + BWI76_RS13735 0.11 -0.0
2,864,412 - BWI76_RS13735 0.13 -0.9
2,864,412 - BWI76_RS13735 0.13 +1.3
2,864,412 - BWI76_RS13735 0.13 -0.4
2,864,441 - BWI76_RS13735 0.18 -1.1
2,864,481 + BWI76_RS13735 0.24 +0.0
2,864,489 - BWI76_RS13735 0.26 +0.0
2,864,938 - -0.4

Or see this region's nucleotide sequence