Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13720 and BWI76_RS13725 overlap by 4 nucleotides BWI76_RS13725 and BWI76_RS13730 overlap by 59 nucleotides BWI76_RS13730 and BWI76_RS13735 are separated by 102 nucleotides BWI76_RS13735 and BWI76_RS13740 are separated by 48 nucleotides
BWI76_RS13720: BWI76_RS13720 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 2,862,462 to 2,863,235
_RS13720
BWI76_RS13725: BWI76_RS13725 - aromatic amino acid exporter, at 2,863,232 to 2,864,110
_RS13725
BWI76_RS13730: BWI76_RS13730 - hypothetical protein, at 2,864,052 to 2,864,235
_RS13730
BWI76_RS13735: BWI76_RS13735 - sulfate ABC transporter substrate-binding protein, at 2,864,338 to 2,864,925
_RS13735
BWI76_RS13740: BWI76_RS13740 - formate dehydrogenase-N subunit alpha, at 2,864,974 to 2,867,385
_RS13740
Position (kb)
2864
2865 Strain fitness (log2 ratio)
-1
0
1 at 2863.137 kb on - strand, within BWI76_RS13720 at 2863.254 kb on - strand at 2863.258 kb on + strand at 2863.322 kb on - strand, within BWI76_RS13725 at 2863.322 kb on - strand, within BWI76_RS13725 at 2863.424 kb on + strand, within BWI76_RS13725 at 2863.437 kb on + strand, within BWI76_RS13725 at 2863.475 kb on + strand, within BWI76_RS13725 at 2863.475 kb on + strand, within BWI76_RS13725 at 2863.483 kb on - strand, within BWI76_RS13725 at 2863.483 kb on - strand, within BWI76_RS13725 at 2863.483 kb on - strand, within BWI76_RS13725 at 2863.522 kb on + strand, within BWI76_RS13725 at 2863.523 kb on + strand, within BWI76_RS13725 at 2863.530 kb on - strand at 2863.535 kb on + strand, within BWI76_RS13725 at 2863.549 kb on + strand, within BWI76_RS13725 at 2863.590 kb on + strand, within BWI76_RS13725 at 2863.677 kb on + strand, within BWI76_RS13725 at 2863.702 kb on + strand, within BWI76_RS13725 at 2863.797 kb on + strand, within BWI76_RS13725 at 2863.826 kb on + strand, within BWI76_RS13725 at 2863.830 kb on + strand, within BWI76_RS13725 at 2863.836 kb on - strand, within BWI76_RS13725 at 2863.840 kb on + strand, within BWI76_RS13725 at 2863.840 kb on + strand, within BWI76_RS13725 at 2863.852 kb on + strand, within BWI76_RS13725 at 2863.855 kb on - strand, within BWI76_RS13725 at 2863.883 kb on - strand, within BWI76_RS13725 at 2863.959 kb on + strand, within BWI76_RS13725 at 2863.991 kb on + strand, within BWI76_RS13725 at 2863.994 kb on - strand, within BWI76_RS13725 at 2864.012 kb on - strand, within BWI76_RS13725 at 2864.020 kb on + strand, within BWI76_RS13725 at 2864.034 kb on + strand at 2864.063 kb on + strand at 2864.063 kb on + strand at 2864.065 kb on + strand at 2864.065 kb on + strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.071 kb on - strand at 2864.073 kb on - strand at 2864.087 kb on - strand at 2864.190 kb on + strand, within BWI76_RS13730 at 2864.198 kb on - strand, within BWI76_RS13730 at 2864.323 kb on + strand at 2864.328 kb on + strand at 2864.336 kb on + strand at 2864.336 kb on - strand at 2864.393 kb on - strand at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.404 kb on + strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.412 kb on - strand, within BWI76_RS13735 at 2864.441 kb on - strand, within BWI76_RS13735 at 2864.481 kb on + strand, within BWI76_RS13735 at 2864.489 kb on - strand, within BWI76_RS13735 at 2864.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,863,137 - BWI76_RS13720 0.87 -1.4 2,863,254 - -0.5 2,863,258 + -0.0 2,863,322 - BWI76_RS13725 0.10 -0.2 2,863,322 - BWI76_RS13725 0.10 -0.2 2,863,424 + BWI76_RS13725 0.22 -0.8 2,863,437 + BWI76_RS13725 0.23 -0.5 2,863,475 + BWI76_RS13725 0.28 -0.0 2,863,475 + BWI76_RS13725 0.28 +0.6 2,863,483 - BWI76_RS13725 0.29 -0.3 2,863,483 - BWI76_RS13725 0.29 -0.9 2,863,483 - BWI76_RS13725 0.29 -0.1 2,863,522 + BWI76_RS13725 0.33 -0.4 2,863,523 + BWI76_RS13725 0.33 -0.8 2,863,530 - -0.4 2,863,535 + BWI76_RS13725 0.34 -0.1 2,863,549 + BWI76_RS13725 0.36 -0.4 2,863,590 + BWI76_RS13725 0.41 -0.2 2,863,677 + BWI76_RS13725 0.51 -1.2 2,863,702 + BWI76_RS13725 0.53 -0.7 2,863,797 + BWI76_RS13725 0.64 -1.0 2,863,826 + BWI76_RS13725 0.68 -0.4 2,863,830 + BWI76_RS13725 0.68 -0.0 2,863,836 - BWI76_RS13725 0.69 -0.1 2,863,840 + BWI76_RS13725 0.69 +0.3 2,863,840 + BWI76_RS13725 0.69 -0.2 2,863,852 + BWI76_RS13725 0.71 -0.6 2,863,855 - BWI76_RS13725 0.71 -0.5 2,863,883 - BWI76_RS13725 0.74 +0.4 2,863,959 + BWI76_RS13725 0.83 -0.1 2,863,991 + BWI76_RS13725 0.86 +0.4 2,863,994 - BWI76_RS13725 0.87 +0.3 2,864,012 - BWI76_RS13725 0.89 +0.4 2,864,020 + BWI76_RS13725 0.90 +0.1 2,864,034 + -0.2 2,864,063 + -0.6 2,864,063 + -0.4 2,864,065 + -0.4 2,864,065 + -0.0 2,864,071 - +0.2 2,864,071 - +0.1 2,864,071 - -0.6 2,864,071 - -0.1 2,864,073 - +0.1 2,864,087 - -0.2 2,864,190 + BWI76_RS13730 0.75 -0.0 2,864,198 - BWI76_RS13730 0.79 +0.4 2,864,323 + +0.4 2,864,328 + -0.3 2,864,336 + -0.6 2,864,336 - -0.9 2,864,393 - -0.1 2,864,404 + BWI76_RS13735 0.11 -0.1 2,864,404 + BWI76_RS13735 0.11 -0.4 2,864,404 + BWI76_RS13735 0.11 +0.2 2,864,404 + BWI76_RS13735 0.11 -0.0 2,864,412 - BWI76_RS13735 0.13 -0.9 2,864,412 - BWI76_RS13735 0.13 +1.3 2,864,412 - BWI76_RS13735 0.13 -0.4 2,864,441 - BWI76_RS13735 0.18 -1.1 2,864,481 + BWI76_RS13735 0.24 +0.0 2,864,489 - BWI76_RS13735 0.26 +0.0 2,864,938 - -0.4
Or see this region's nucleotide sequence