Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13320

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13310 and BWI76_RS13315 are separated by 75 nucleotidesBWI76_RS13315 and BWI76_RS13320 are separated by 95 nucleotidesBWI76_RS13320 and BWI76_RS13325 are separated by 110 nucleotides BWI76_RS13310: BWI76_RS13310 - glucan biosynthesis protein D, at 2,775,559 to 2,777,214 _RS13310 BWI76_RS13315: BWI76_RS13315 - 50S ribosomal protein L7/L12-serine acetyltransferase, at 2,777,290 to 2,777,874 _RS13315 BWI76_RS13320: BWI76_RS13320 - putative LpxA-like enzyme, at 2,777,970 to 2,778,950 _RS13320 BWI76_RS13325: BWI76_RS13325 - dicarboxylate transporter/tellurite-resistance protein TehA, at 2,779,061 to 2,780,059 _RS13325 Position (kb) 2777 2778 2779Strain fitness (log2 ratio) -1 0 1at 2777.103 kb on - strandat 2777.126 kb on - strandat 2777.128 kb on - strandat 2777.367 kb on - strand, within BWI76_RS13315at 2778.638 kb on + strand, within BWI76_RS13320at 2778.797 kb on - strand, within BWI76_RS13320at 2778.887 kb on + strandat 2778.887 kb on + strandat 2778.901 kb on + strandat 2779.143 kb on + strandat 2779.202 kb on + strand, within BWI76_RS13325at 2779.278 kb on + strand, within BWI76_RS13325at 2779.286 kb on - strand, within BWI76_RS13325at 2779.346 kb on - strand, within BWI76_RS13325at 2779.605 kb on + strand, within BWI76_RS13325

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,777,103 - -0.3
2,777,126 - +0.0
2,777,128 - -0.2
2,777,367 - BWI76_RS13315 0.13 -0.1
2,778,638 + BWI76_RS13320 0.68 +0.0
2,778,797 - BWI76_RS13320 0.84 +0.6
2,778,887 + -0.5
2,778,887 + -0.2
2,778,901 + +0.8
2,779,143 + -0.2
2,779,202 + BWI76_RS13325 0.14 -0.0
2,779,278 + BWI76_RS13325 0.22 -0.8
2,779,286 - BWI76_RS13325 0.23 -0.5
2,779,346 - BWI76_RS13325 0.29 +1.4
2,779,605 + BWI76_RS13325 0.54 +0.1

Or see this region's nucleotide sequence