Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotides BWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotides BWI76_RS10540 and BWI76_RS10545 are separated by 227 nucleotides BWI76_RS10545 and BWI76_RS10550 overlap by 1 nucleotides
BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169
_RS10530
BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912
_RS10535
BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861
_RS10540
BWI76_RS10545: BWI76_RS10545 - hypothetical protein, at 2,228,089 to 2,228,472
_RS10545
BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869
_RS10550
Position (kb)
2226
2227
2228 Strain fitness (log2 ratio)
-2
-1
0
1 at 2225.939 kb on + strand, within BWI76_RS10530 at 2226.330 kb on - strand, within BWI76_RS10535 at 2226.357 kb on + strand, within BWI76_RS10535 at 2226.595 kb on - strand, within BWI76_RS10535 at 2226.975 kb on + strand at 2227.349 kb on + strand, within BWI76_RS10540 at 2227.392 kb on - strand, within BWI76_RS10540 at 2227.618 kb on - strand, within BWI76_RS10540 at 2227.644 kb on - strand, within BWI76_RS10540 at 2227.667 kb on - strand, within BWI76_RS10540 at 2227.754 kb on - strand, within BWI76_RS10540 at 2227.888 kb on + strand at 2227.909 kb on - strand at 2228.048 kb on + strand at 2228.077 kb on - strand at 2228.117 kb on - strand at 2228.716 kb on - strand, within BWI76_RS10550
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,225,939 + BWI76_RS10530 0.68 +0.1 2,226,330 - BWI76_RS10535 0.22 -0.5 2,226,357 + BWI76_RS10535 0.25 -0.4 2,226,595 - BWI76_RS10535 0.57 -0.2 2,226,975 + +0.1 2,227,349 + BWI76_RS10540 0.45 -2.2 2,227,392 - BWI76_RS10540 0.50 +0.6 2,227,618 - BWI76_RS10540 0.74 +0.2 2,227,644 - BWI76_RS10540 0.77 +0.4 2,227,667 - BWI76_RS10540 0.79 +1.3 2,227,754 - BWI76_RS10540 0.88 -0.2 2,227,888 + -0.5 2,227,909 - -0.8 2,228,048 + -0.7 2,228,077 - +0.4 2,228,117 - -0.4 2,228,716 - BWI76_RS10550 0.17 -0.2
Or see this region's nucleotide sequence