Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10540

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotidesBWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotidesBWI76_RS10540 and BWI76_RS10545 are separated by 227 nucleotidesBWI76_RS10545 and BWI76_RS10550 overlap by 1 nucleotides BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169 _RS10530 BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912 _RS10535 BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861 _RS10540 BWI76_RS10545: BWI76_RS10545 - hypothetical protein, at 2,228,089 to 2,228,472 _RS10545 BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869 _RS10550 Position (kb) 2226 2227 2228Strain fitness (log2 ratio) -2 -1 0 1at 2225.939 kb on + strand, within BWI76_RS10530at 2226.330 kb on - strand, within BWI76_RS10535at 2226.357 kb on + strand, within BWI76_RS10535at 2226.595 kb on - strand, within BWI76_RS10535at 2226.975 kb on + strandat 2227.349 kb on + strand, within BWI76_RS10540at 2227.392 kb on - strand, within BWI76_RS10540at 2227.618 kb on - strand, within BWI76_RS10540at 2227.644 kb on - strand, within BWI76_RS10540at 2227.667 kb on - strand, within BWI76_RS10540at 2227.754 kb on - strand, within BWI76_RS10540at 2227.888 kb on + strandat 2227.909 kb on - strandat 2228.048 kb on + strandat 2228.077 kb on - strandat 2228.117 kb on - strandat 2228.716 kb on - strand, within BWI76_RS10550

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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2,225,939 + BWI76_RS10530 0.68 +0.1
2,226,330 - BWI76_RS10535 0.22 -0.5
2,226,357 + BWI76_RS10535 0.25 -0.4
2,226,595 - BWI76_RS10535 0.57 -0.2
2,226,975 + +0.1
2,227,349 + BWI76_RS10540 0.45 -2.2
2,227,392 - BWI76_RS10540 0.50 +0.6
2,227,618 - BWI76_RS10540 0.74 +0.2
2,227,644 - BWI76_RS10540 0.77 +0.4
2,227,667 - BWI76_RS10540 0.79 +1.3
2,227,754 - BWI76_RS10540 0.88 -0.2
2,227,888 + -0.5
2,227,909 - -0.8
2,228,048 + -0.7
2,228,077 - +0.4
2,228,117 - -0.4
2,228,716 - BWI76_RS10550 0.17 -0.2

Or see this region's nucleotide sequence