Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10395 and BWI76_RS10400 are separated by 355 nucleotides BWI76_RS10400 and BWI76_RS10405 are separated by 125 nucleotides BWI76_RS10405 and BWI76_RS10410 are separated by 234 nucleotides BWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotides
BWI76_RS10395: BWI76_RS10395 - cold-shock protein, at 2,201,107 to 2,201,319
_RS10395
BWI76_RS10400: BWI76_RS10400 - hypothetical protein, at 2,201,675 to 2,201,860
_RS10400
BWI76_RS10405: BWI76_RS10405 - cold-shock protein, at 2,201,986 to 2,202,207
_RS10405
BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702
_RS10410
BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171
_RS10415
Position (kb)
2201
2202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2200.694 kb on - strand at 2200.694 kb on - strand at 2200.784 kb on - strand at 2200.866 kb on + strand at 2200.875 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2200.975 kb on + strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.067 kb on - strand at 2201.085 kb on - strand at 2201.114 kb on + strand at 2201.114 kb on + strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.122 kb on - strand at 2201.127 kb on - strand at 2201.178 kb on - strand, within BWI76_RS10395 at 2201.183 kb on - strand, within BWI76_RS10395 at 2201.190 kb on - strand, within BWI76_RS10395 at 2201.248 kb on + strand at 2201.255 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.256 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.270 kb on - strand, within BWI76_RS10395 at 2201.288 kb on - strand, within BWI76_RS10395 at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.344 kb on - strand at 2201.345 kb on - strand at 2201.345 kb on - strand at 2201.379 kb on - strand at 2201.425 kb on + strand at 2201.425 kb on + strand at 2201.427 kb on + strand at 2201.433 kb on - strand at 2201.471 kb on + strand at 2201.471 kb on + strand at 2201.550 kb on + strand at 2201.573 kb on + strand at 2201.584 kb on + strand at 2201.591 kb on + strand at 2201.639 kb on + strand at 2201.698 kb on + strand, within BWI76_RS10400 at 2201.698 kb on + strand, within BWI76_RS10400 at 2201.706 kb on - strand, within BWI76_RS10400 at 2201.711 kb on - strand, within BWI76_RS10400 at 2201.724 kb on - strand, within BWI76_RS10400 at 2201.765 kb on + strand, within BWI76_RS10400 at 2201.877 kb on + strand at 2201.933 kb on + strand at 2201.936 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.965 kb on - strand at 2201.972 kb on + strand at 2201.986 kb on - strand at 2202.004 kb on + strand at 2202.007 kb on + strand at 2202.012 kb on - strand, within BWI76_RS10405 at 2202.017 kb on + strand, within BWI76_RS10405 at 2202.025 kb on - strand, within BWI76_RS10405 at 2202.027 kb on + strand, within BWI76_RS10405 at 2202.071 kb on - strand, within BWI76_RS10405 at 2202.082 kb on - strand, within BWI76_RS10405 at 2202.084 kb on + strand, within BWI76_RS10405 at 2202.086 kb on + strand, within BWI76_RS10405 at 2202.150 kb on + strand, within BWI76_RS10405 at 2202.153 kb on + strand, within BWI76_RS10405 at 2202.160 kb on + strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.170 kb on + strand, within BWI76_RS10405 at 2202.171 kb on - strand, within BWI76_RS10405 at 2202.175 kb on + strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.241 kb on - strand at 2202.241 kb on - strand at 2202.254 kb on + strand at 2202.268 kb on + strand at 2202.273 kb on + strand at 2202.284 kb on + strand at 2202.299 kb on - strand at 2202.309 kb on + strand at 2202.309 kb on + strand at 2202.389 kb on + strand at 2202.394 kb on + strand at 2202.394 kb on + strand at 2202.442 kb on - strand at 2202.498 kb on - strand, within BWI76_RS10410 at 2202.610 kb on - strand, within BWI76_RS10410 at 2202.806 kb on - strand at 2202.835 kb on + strand, within BWI76_RS10415 at 2202.845 kb on + strand, within BWI76_RS10415
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,200,694 - -0.5 2,200,694 - +0.7 2,200,784 - -0.6 2,200,866 + +2.1 2,200,875 + +0.6 2,200,975 + -0.3 2,200,975 + -0.0 2,200,975 + -0.6 2,200,975 + +0.2 2,200,975 + -0.2 2,201,056 - -0.5 2,201,056 - -0.1 2,201,056 - -0.4 2,201,067 - -0.2 2,201,085 - +0.3 2,201,114 + +0.6 2,201,114 + +0.3 2,201,122 - +0.8 2,201,122 - -0.0 2,201,122 - -0.0 2,201,122 - -3.3 2,201,122 - +0.0 2,201,122 - +0.1 2,201,122 - -0.4 2,201,122 - +0.3 2,201,122 - -0.7 2,201,122 - -2.1 2,201,122 - -0.4 2,201,122 - +0.2 2,201,122 - +0.1 2,201,122 - -0.6 2,201,127 - -0.1 2,201,178 - BWI76_RS10395 0.33 +0.3 2,201,183 - BWI76_RS10395 0.36 -0.4 2,201,190 - BWI76_RS10395 0.39 +0.0 2,201,248 + +0.1 2,201,255 - BWI76_RS10395 0.69 +0.3 2,201,256 - BWI76_RS10395 0.70 +0.3 2,201,256 - BWI76_RS10395 0.70 -0.1 2,201,270 - -0.4 2,201,270 - BWI76_RS10395 0.77 -1.6 2,201,270 - BWI76_RS10395 0.77 -0.3 2,201,288 - BWI76_RS10395 0.85 +0.1 2,201,344 - +1.1 2,201,344 - -0.1 2,201,344 - -0.4 2,201,345 - -0.5 2,201,345 - +0.5 2,201,379 - -0.6 2,201,425 + +0.5 2,201,425 + +0.3 2,201,427 + -0.9 2,201,433 - -0.4 2,201,471 + +0.1 2,201,471 + -0.0 2,201,550 + -0.7 2,201,573 + -0.7 2,201,584 + -1.5 2,201,591 + +1.0 2,201,639 + -1.3 2,201,698 + BWI76_RS10400 0.12 +1.0 2,201,698 + BWI76_RS10400 0.12 +0.2 2,201,706 - BWI76_RS10400 0.17 +0.9 2,201,711 - BWI76_RS10400 0.19 +0.4 2,201,724 - BWI76_RS10400 0.26 +0.0 2,201,765 + BWI76_RS10400 0.48 +0.0 2,201,877 + +0.1 2,201,933 + -0.3 2,201,936 - +1.1 2,201,941 - +0.0 2,201,941 - +0.3 2,201,941 - +0.2 2,201,941 - -0.3 2,201,965 - +0.5 2,201,972 + -0.1 2,201,986 - -0.1 2,202,004 + -0.3 2,202,007 + -1.8 2,202,012 - BWI76_RS10405 0.12 -0.6 2,202,017 + BWI76_RS10405 0.14 +1.9 2,202,025 - BWI76_RS10405 0.18 +1.1 2,202,027 + BWI76_RS10405 0.18 +0.9 2,202,071 - BWI76_RS10405 0.38 -0.0 2,202,082 - BWI76_RS10405 0.43 +0.0 2,202,084 + BWI76_RS10405 0.44 -0.0 2,202,086 + BWI76_RS10405 0.45 -0.0 2,202,150 + BWI76_RS10405 0.74 -0.1 2,202,153 + BWI76_RS10405 0.75 -0.7 2,202,160 + BWI76_RS10405 0.78 -0.1 2,202,161 - BWI76_RS10405 0.79 +0.1 2,202,161 - BWI76_RS10405 0.79 -0.7 2,202,170 + BWI76_RS10405 0.83 +0.2 2,202,171 - BWI76_RS10405 0.83 -1.4 2,202,175 + BWI76_RS10405 0.85 -0.4 2,202,183 - BWI76_RS10405 0.89 -0.3 2,202,183 - BWI76_RS10405 0.89 -0.4 2,202,185 + +0.8 2,202,185 + -0.9 2,202,185 + -0.2 2,202,185 + -0.3 2,202,185 + -1.0 2,202,185 + -0.7 2,202,185 + +0.1 2,202,193 - -0.2 2,202,193 - -0.4 2,202,193 - -0.2 2,202,193 - -0.1 2,202,193 - -0.4 2,202,193 - -0.4 2,202,193 - -0.6 2,202,193 - -0.3 2,202,193 - -0.5 2,202,193 - +0.2 2,202,193 - +0.1 2,202,193 - -0.1 2,202,193 - -0.4 2,202,233 + +0.3 2,202,233 + -0.4 2,202,233 + +1.0 2,202,233 + -1.3 2,202,233 + -0.1 2,202,241 - -0.1 2,202,241 - -1.0 2,202,254 + -0.2 2,202,268 + -0.9 2,202,273 + -0.5 2,202,284 + +0.2 2,202,299 - -0.7 2,202,309 + +0.1 2,202,309 + -0.7 2,202,389 + -0.2 2,202,394 + -0.1 2,202,394 + +0.2 2,202,442 - -0.2 2,202,498 - BWI76_RS10410 0.21 +0.3 2,202,610 - BWI76_RS10410 0.64 -0.4 2,202,806 - +0.2 2,202,835 + BWI76_RS10415 0.11 -0.0 2,202,845 + BWI76_RS10415 0.13 -0.2
Or see this region's nucleotide sequence