Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09600 and BWI76_RS09605 are separated by 68 nucleotides BWI76_RS09605 and BWI76_RS09610 are separated by 324 nucleotides BWI76_RS09610 and BWI76_RS09615 are separated by 30 nucleotides BWI76_RS09615 and BWI76_RS09620 are separated by 138 nucleotides BWI76_RS09620 and BWI76_RS09625 overlap by 73 nucleotides BWI76_RS09625 and BWI76_RS09630 are separated by 0 nucleotides
BWI76_RS09600: BWI76_RS09600 - macrolide ABC transporter permease/ATP-binding protein MacB, at 2,008,593 to 2,010,539
_RS09600
BWI76_RS09605: BWI76_RS09605 - cold-shock protein, at 2,010,608 to 2,010,838
_RS09605
BWI76_RS09610: BWI76_RS09610 - ATP-dependent Clp protease adaptor ClpS, at 2,011,163 to 2,011,480
_RS09610
BWI76_RS09615: BWI76_RS09615 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,011,511 to 2,013,793
_RS09615
BWI76_RS09620: BWI76_RS09620 - translation initiation factor IF-1, at 2,013,932 to 2,014,150
_RS09620
BWI76_RS09625: BWI76_RS09625 - hypothetical protein, at 2,014,078 to 2,014,429
_RS09625
BWI76_RS09630: BWI76_RS09630 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,014,430 to 2,015,134
_RS09630
Position (kb)
2011
2012
2013
2014 Strain fitness (log2 ratio)
-2
-1
0
1 at 2010.584 kb on + strand at 2010.592 kb on - strand at 2010.832 kb on + strand at 2011.146 kb on - strand at 2011.165 kb on + strand at 2011.267 kb on - strand, within BWI76_RS09610 at 2011.337 kb on - strand, within BWI76_RS09610 at 2011.456 kb on + strand at 2011.579 kb on + strand at 2011.612 kb on + strand at 2012.273 kb on + strand, within BWI76_RS09615 at 2012.492 kb on + strand, within BWI76_RS09615 at 2013.343 kb on + strand, within BWI76_RS09615 at 2013.483 kb on - strand, within BWI76_RS09615 at 2014.300 kb on + strand, within BWI76_RS09625
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 2,010,584 + -0.2 2,010,592 - +0.2 2,010,832 + +0.1 2,011,146 - -0.0 2,011,165 + -0.0 2,011,267 - BWI76_RS09610 0.33 -1.8 2,011,337 - BWI76_RS09610 0.55 -1.0 2,011,456 + -0.2 2,011,579 + -1.7 2,011,612 + +0.1 2,012,273 + BWI76_RS09615 0.33 -1.4 2,012,492 + BWI76_RS09615 0.43 +0.5 2,013,343 + BWI76_RS09615 0.80 +0.9 2,013,483 - BWI76_RS09615 0.86 +0.6 2,014,300 + BWI76_RS09625 0.63 +0.1
Or see this region's nucleotide sequence