Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08865

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08855 and BWI76_RS08860 are separated by 143 nucleotidesBWI76_RS08860 and BWI76_RS08865 overlap by 39 nucleotidesBWI76_RS08865 and BWI76_RS08870 are separated by 121 nucleotides BWI76_RS08855: BWI76_RS08855 - endonuclease/exonuclease/phosphatase, at 1,864,368 to 1,865,117 _RS08855 BWI76_RS08860: BWI76_RS08860 - hypothetical protein, at 1,865,261 to 1,865,671 _RS08860 BWI76_RS08865: BWI76_RS08865 - ABC transporter inner membrane, at 1,865,633 to 1,866,739 _RS08865 BWI76_RS08870: BWI76_RS08870 - inner membrane transport permease YbhS, at 1,866,861 to 1,867,994 _RS08870 Position (kb) 1865 1866 1867Strain fitness (log2 ratio) -1 0 1at 1864.634 kb on + strand, within BWI76_RS08855at 1865.569 kb on - strand, within BWI76_RS08860at 1866.259 kb on + strand, within BWI76_RS08865at 1866.299 kb on - strand, within BWI76_RS08865at 1866.558 kb on - strand, within BWI76_RS08865at 1866.639 kb on + strandat 1866.698 kb on + strandat 1866.728 kb on - strandat 1866.775 kb on + strandat 1866.775 kb on + strandat 1866.783 kb on - strandat 1866.790 kb on + strandat 1867.120 kb on + strand, within BWI76_RS08870at 1867.171 kb on - strand, within BWI76_RS08870at 1867.263 kb on + strand, within BWI76_RS08870at 1867.263 kb on - strand, within BWI76_RS08870at 1867.395 kb on + strand, within BWI76_RS08870at 1867.403 kb on - strand, within BWI76_RS08870at 1867.480 kb on - strand, within BWI76_RS08870at 1867.575 kb on + strand, within BWI76_RS08870

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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1,864,634 + BWI76_RS08855 0.35 +0.2
1,865,569 - BWI76_RS08860 0.75 -0.1
1,866,259 + BWI76_RS08865 0.57 +0.2
1,866,299 - BWI76_RS08865 0.60 +0.2
1,866,558 - BWI76_RS08865 0.84 +0.0
1,866,639 + -0.1
1,866,698 + +0.2
1,866,728 - -0.1
1,866,775 + +0.1
1,866,775 + +0.2
1,866,783 - -0.1
1,866,790 + -0.1
1,867,120 + BWI76_RS08870 0.23 +0.0
1,867,171 - BWI76_RS08870 0.27 +0.9
1,867,263 + BWI76_RS08870 0.35 +0.1
1,867,263 - BWI76_RS08870 0.35 +0.3
1,867,395 + BWI76_RS08870 0.47 +1.3
1,867,403 - BWI76_RS08870 0.48 +0.1
1,867,480 - BWI76_RS08870 0.55 +0.5
1,867,575 + BWI76_RS08870 0.63 -0.3

Or see this region's nucleotide sequence