Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06425 and BWI76_RS06430 overlap by 2 nucleotides BWI76_RS06430 and BWI76_RS06435 are separated by 229 nucleotides BWI76_RS06435 and BWI76_RS06440 are separated by 10 nucleotides BWI76_RS06440 and BWI76_RS06445 are separated by 65 nucleotides
BWI76_RS06425: BWI76_RS06425 - MFS transporter, at 1,350,342 to 1,351,742
_RS06425
BWI76_RS06430: BWI76_RS06430 - hypothetical protein, at 1,351,741 to 1,351,920
_RS06430
BWI76_RS06435: BWI76_RS06435 - MFS transporter, at 1,352,150 to 1,353,514
_RS06435
BWI76_RS06440: BWI76_RS06440 - AP endonuclease, at 1,353,525 to 1,354,343
_RS06440
BWI76_RS06445: BWI76_RS06445 - FAD-dependent oxidoreductase, at 1,354,409 to 1,356,112
_RS06445
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-1
0
1
2 at 1351.164 kb on - strand, within BWI76_RS06425 at 1351.189 kb on + strand, within BWI76_RS06425 at 1351.290 kb on + strand, within BWI76_RS06425 at 1351.549 kb on - strand, within BWI76_RS06425 at 1351.605 kb on - strand at 1351.769 kb on + strand at 1351.770 kb on + strand, within BWI76_RS06430 at 1351.778 kb on - strand, within BWI76_RS06430 at 1351.788 kb on - strand, within BWI76_RS06430 at 1351.802 kb on + strand, within BWI76_RS06430 at 1351.802 kb on + strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.942 kb on - strand at 1351.944 kb on - strand at 1351.959 kb on + strand at 1352.168 kb on - strand at 1352.391 kb on + strand, within BWI76_RS06435 at 1352.435 kb on + strand, within BWI76_RS06435 at 1352.564 kb on - strand, within BWI76_RS06435 at 1353.498 kb on - strand at 1354.045 kb on + strand, within BWI76_RS06440 at 1354.360 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 1,351,164 - BWI76_RS06425 0.59 -0.1 1,351,189 + BWI76_RS06425 0.60 -0.0 1,351,290 + BWI76_RS06425 0.68 -0.2 1,351,549 - BWI76_RS06425 0.86 -0.1 1,351,605 - +0.2 1,351,769 + -0.2 1,351,770 + BWI76_RS06430 0.16 -0.2 1,351,778 - BWI76_RS06430 0.21 +0.4 1,351,788 - BWI76_RS06430 0.26 -1.2 1,351,802 + BWI76_RS06430 0.34 -0.2 1,351,802 + BWI76_RS06430 0.34 -0.2 1,351,810 - BWI76_RS06430 0.38 -0.7 1,351,810 - BWI76_RS06430 0.38 -0.2 1,351,810 - BWI76_RS06430 0.38 -0.2 1,351,942 - -0.3 1,351,944 - -0.3 1,351,959 + +0.1 1,352,168 - +0.2 1,352,391 + BWI76_RS06435 0.18 +2.1 1,352,435 + BWI76_RS06435 0.21 +0.2 1,352,564 - BWI76_RS06435 0.30 +0.1 1,353,498 - -0.4 1,354,045 + BWI76_RS06440 0.63 -0.1 1,354,360 + -0.5
Or see this region's nucleotide sequence