Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05475 and BWI76_RS05480 are separated by 43 nucleotides BWI76_RS05480 and BWI76_RS05485 overlap by 30 nucleotides BWI76_RS05485 and BWI76_RS05490 are separated by 413 nucleotides
BWI76_RS05475: BWI76_RS05475 - phosphoheptose isomerase, at 1,149,317 to 1,149,898
_RS05475
BWI76_RS05480: BWI76_RS05480 - class II glutamine amidotransferase, at 1,149,942 to 1,150,709
_RS05480
BWI76_RS05485: BWI76_RS05485 - transpeptidase, at 1,150,680 to 1,151,420
_RS05485
BWI76_RS05490: BWI76_RS05490 - Na(+)-translocating NADH-quinone reductase subunit A, at 1,151,834 to 1,153,177
_RS05490
Position (kb) 1150
1151
1152 Strain fitness (log2 ratio) -1
0
1 at 1150.067 kb on + strand, within BWI76_RS05480 at 1150.068 kb on + strand, within BWI76_RS05480 at 1150.076 kb on - strand, within BWI76_RS05480 at 1150.252 kb on - strand, within BWI76_RS05480 at 1150.474 kb on - strand, within BWI76_RS05480 at 1151.107 kb on + strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.186 kb on + strand, within BWI76_RS05485 at 1151.214 kb on + strand, within BWI76_RS05485 at 1151.284 kb on + strand, within BWI76_RS05485 at 1151.299 kb on + strand, within BWI76_RS05485 at 1151.341 kb on - strand, within BWI76_RS05485 at 1151.518 kb on - strand at 1151.699 kb on + strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.944 kb on - strand at 1151.988 kb on + strand, within BWI76_RS05490
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 1,150,067 + BWI76_RS05480 0.16 +0.2 1,150,068 + BWI76_RS05480 0.16 -0.5 1,150,076 - BWI76_RS05480 0.17 +0.1 1,150,252 - BWI76_RS05480 0.40 -0.2 1,150,474 - BWI76_RS05480 0.69 -0.1 1,151,107 + BWI76_RS05485 0.58 -0.3 1,151,179 - BWI76_RS05485 0.67 +0.1 1,151,179 - BWI76_RS05485 0.67 -0.0 1,151,186 + BWI76_RS05485 0.68 +0.4 1,151,214 + BWI76_RS05485 0.72 +0.8 1,151,284 + BWI76_RS05485 0.82 +0.3 1,151,299 + BWI76_RS05485 0.84 +0.1 1,151,341 - BWI76_RS05485 0.89 -0.1 1,151,518 - -0.8 1,151,699 + +0.4 1,151,707 - -0.1 1,151,707 - +0.4 1,151,707 - -0.1 1,151,707 - +0.1 1,151,944 - +0.2 1,151,988 + BWI76_RS05490 0.11 -0.6
Or see this region's nucleotide sequence