Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05040 and BWI76_RS05045 overlap by 11 nucleotides BWI76_RS05045 and BWI76_RS05050 overlap by 8 nucleotides BWI76_RS05050 and BWI76_RS05055 overlap by 8 nucleotides BWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotides
BWI76_RS05040: BWI76_RS05040 - type II secretion system protein N, at 1,057,283 to 1,058,041
_RS05040
BWI76_RS05045: BWI76_RS05045 - general secretion pathway protein GspM, at 1,058,031 to 1,058,516
_RS05045
BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705
_RS05050
BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678
_RS05055
BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271
_RS05060
Position (kb)
1058
1059
1060 Strain fitness (log2 ratio)
-2
-1
0
1 at 1057.520 kb on + strand, within BWI76_RS05040 at 1057.946 kb on - strand, within BWI76_RS05040 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.541 kb on + strand at 1058.616 kb on + strand at 1058.624 kb on - strand at 1058.624 kb on - strand at 1059.018 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050 at 1059.533 kb on - strand, within BWI76_RS05050 at 1059.797 kb on + strand, within BWI76_RS05055 at 1059.970 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.106 kb on - strand, within BWI76_RS05055 at 1060.181 kb on - strand, within BWI76_RS05055 at 1060.242 kb on + strand, within BWI76_RS05055 at 1060.267 kb on + strand, within BWI76_RS05055 at 1060.311 kb on + strand, within BWI76_RS05055 at 1060.348 kb on + strand, within BWI76_RS05055 at 1060.371 kb on - strand, within BWI76_RS05055 at 1060.438 kb on + strand at 1060.445 kb on + strand, within BWI76_RS05055 at 1060.453 kb on - strand, within BWI76_RS05055 at 1060.607 kb on + strand at 1060.649 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 1,057,520 + BWI76_RS05040 0.31 -0.3 1,057,946 - BWI76_RS05040 0.87 +0.9 1,058,457 - BWI76_RS05045 0.88 -2.7 1,058,457 - BWI76_RS05045 0.88 -0.3 1,058,541 + +0.9 1,058,616 + +0.6 1,058,624 - -0.2 1,058,624 - +0.3 1,059,018 + BWI76_RS05050 0.43 +1.1 1,059,306 + BWI76_RS05050 0.67 +0.9 1,059,306 + BWI76_RS05050 0.67 +0.8 1,059,533 - BWI76_RS05050 0.86 -0.3 1,059,797 + BWI76_RS05055 0.10 -0.0 1,059,970 + BWI76_RS05055 0.28 -0.4 1,060,020 + BWI76_RS05055 0.33 +0.0 1,060,020 + BWI76_RS05055 0.33 -0.9 1,060,020 + BWI76_RS05055 0.33 -0.3 1,060,106 - BWI76_RS05055 0.42 -0.3 1,060,181 - BWI76_RS05055 0.49 -0.1 1,060,242 + BWI76_RS05055 0.55 -0.2 1,060,267 + BWI76_RS05055 0.58 -0.0 1,060,311 + BWI76_RS05055 0.62 +0.5 1,060,348 + BWI76_RS05055 0.66 +0.2 1,060,371 - BWI76_RS05055 0.69 -0.2 1,060,438 + +0.1 1,060,445 + BWI76_RS05055 0.76 +0.1 1,060,453 - BWI76_RS05055 0.77 -0.1 1,060,607 + -0.2 1,060,649 - -0.3
Or see this region's nucleotide sequence