Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05015

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotidesBWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotidesBWI76_RS05015 and BWI76_RS05020 are separated by 164 nucleotidesBWI76_RS05020 and BWI76_RS05025 overlap by 1 nucleotides BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632 _RS05005 BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565 _RS05010 BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082 _RS05015 BWI76_RS05020: BWI76_RS05020 - sugar fermentation stimulation protein SfsA, at 1,052,247 to 1,052,963 _RS05020 BWI76_RS05025: BWI76_RS05025 - 2'-5' RNA ligase, at 1,052,963 to 1,053,499 _RS05025 Position (kb) 1051 1052 1053Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1050.692 kb on - strandat 1050.692 kb on - strandat 1050.761 kb on + strandat 1050.844 kb on + strand, within BWI76_RS05010at 1050.844 kb on + strand, within BWI76_RS05010at 1050.852 kb on - strand, within BWI76_RS05010at 1051.312 kb on - strand, within BWI76_RS05010at 1051.541 kb on + strandat 1051.571 kb on + strandat 1051.676 kb on + strand, within BWI76_RS05015at 1051.867 kb on - strand, within BWI76_RS05015at 1051.894 kb on - strand, within BWI76_RS05015at 1051.955 kb on - strand, within BWI76_RS05015at 1052.065 kb on + strandat 1052.187 kb on - strandat 1052.187 kb on - strandat 1052.187 kb on - strandat 1052.195 kb on - strandat 1052.195 kb on - strandat 1052.197 kb on - strandat 1052.264 kb on + strandat 1052.286 kb on - strandat 1052.291 kb on - strandat 1052.330 kb on + strand, within BWI76_RS05020at 1052.381 kb on - strand, within BWI76_RS05020at 1052.382 kb on + strand, within BWI76_RS05020at 1052.390 kb on - strand, within BWI76_RS05020at 1052.395 kb on + strand, within BWI76_RS05020at 1052.395 kb on + strand, within BWI76_RS05020at 1052.426 kb on - strand, within BWI76_RS05020at 1052.524 kb on + strand, within BWI76_RS05020at 1052.531 kb on - strand, within BWI76_RS05020at 1052.543 kb on + strand, within BWI76_RS05020at 1052.656 kb on + strand, within BWI76_RS05020at 1052.779 kb on - strand, within BWI76_RS05020at 1052.809 kb on - strand, within BWI76_RS05020at 1052.823 kb on - strand, within BWI76_RS05020at 1052.851 kb on + strand, within BWI76_RS05020at 1052.860 kb on - strand, within BWI76_RS05020at 1052.862 kb on + strand, within BWI76_RS05020at 1052.871 kb on - strand, within BWI76_RS05020at 1052.881 kb on - strand, within BWI76_RS05020

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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1,050,692 - -0.2
1,050,692 - -0.0
1,050,761 + -0.2
1,050,844 + BWI76_RS05010 0.18 -0.4
1,050,844 + BWI76_RS05010 0.18 +0.3
1,050,852 - BWI76_RS05010 0.19 -0.4
1,051,312 - BWI76_RS05010 0.71 -0.1
1,051,541 + +0.8
1,051,571 + -0.1
1,051,676 + BWI76_RS05015 0.11 -2.4
1,051,867 - BWI76_RS05015 0.53 -2.4
1,051,894 - BWI76_RS05015 0.59 -3.2
1,051,955 - BWI76_RS05015 0.72 -1.2
1,052,065 + -3.8
1,052,187 - -0.0
1,052,187 - -1.0
1,052,187 - -0.0
1,052,195 - -0.7
1,052,195 - +0.2
1,052,197 - -0.3
1,052,264 + +1.5
1,052,286 - +0.0
1,052,291 - -0.4
1,052,330 + BWI76_RS05020 0.12 +1.7
1,052,381 - BWI76_RS05020 0.19 -0.4
1,052,382 + BWI76_RS05020 0.19 -0.0
1,052,390 - BWI76_RS05020 0.20 -0.2
1,052,395 + BWI76_RS05020 0.21 +0.2
1,052,395 + BWI76_RS05020 0.21 -0.1
1,052,426 - BWI76_RS05020 0.25 -0.6
1,052,524 + BWI76_RS05020 0.39 +0.7
1,052,531 - BWI76_RS05020 0.40 -0.1
1,052,543 + BWI76_RS05020 0.41 +0.3
1,052,656 + BWI76_RS05020 0.57 -1.1
1,052,779 - BWI76_RS05020 0.74 -0.2
1,052,809 - BWI76_RS05020 0.78 -0.8
1,052,823 - BWI76_RS05020 0.80 -0.0
1,052,851 + BWI76_RS05020 0.84 -0.1
1,052,860 - BWI76_RS05020 0.85 -0.2
1,052,862 + BWI76_RS05020 0.86 +0.0
1,052,871 - BWI76_RS05020 0.87 -0.0
1,052,881 - BWI76_RS05020 0.88 -0.3

Or see this region's nucleotide sequence