Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02995 and BWI76_RS03000 are separated by 36 nucleotides BWI76_RS03000 and BWI76_RS03005 are separated by 192 nucleotides BWI76_RS03005 and BWI76_RS03010 are separated by 190 nucleotides
BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921
_RS02995
BWI76_RS03000: BWI76_RS03000 - PyrBI operon leader peptide, at 614,958 to 615,062
_RS03000
BWI76_RS03005: BWI76_RS03005 - hypothetical protein, at 615,255 to 615,707
_RS03005
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
Position (kb) 615
616 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 614.303 kb on - strand, within BWI76_RS02995 at 614.318 kb on + strand, within BWI76_RS02995 at 614.407 kb on - strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.477 kb on - strand, within BWI76_RS02995 at 614.488 kb on - strand, within BWI76_RS02995 at 614.503 kb on - strand, within BWI76_RS02995 at 614.557 kb on - strand, within BWI76_RS02995 at 614.919 kb on - strand at 615.106 kb on - strand at 615.140 kb on - strand at 615.140 kb on - strand at 615.279 kb on + strand at 615.300 kb on + strand at 615.323 kb on + strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.356 kb on + strand, within BWI76_RS03005 at 615.617 kb on + strand, within BWI76_RS03005 at 615.682 kb on - strand at 615.739 kb on - strand at 615.800 kb on - strand at 615.823 kb on + strand at 615.829 kb on - strand at 615.858 kb on + strand at 616.124 kb on - strand, within BWI76_RS03010 at 616.214 kb on - strand, within BWI76_RS03010 at 616.216 kb on + strand, within BWI76_RS03010 at 616.224 kb on - strand, within BWI76_RS03010 at 616.361 kb on - strand, within BWI76_RS03010 at 616.361 kb on - strand, within BWI76_RS03010 at 616.388 kb on + strand, within BWI76_RS03010 at 616.423 kb on + strand, within BWI76_RS03010 at 616.431 kb on - strand, within BWI76_RS03010 at 616.480 kb on - strand, within BWI76_RS03010 at 616.480 kb on - strand, within BWI76_RS03010 at 616.489 kb on + strand, within BWI76_RS03010 at 616.507 kb on - strand, within BWI76_RS03010 at 616.530 kb on + strand, within BWI76_RS03010 at 616.695 kb on + strand, within BWI76_RS03010
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 614,303 - BWI76_RS02995 0.34 -3.4 614,318 + BWI76_RS02995 0.35 -5.1 614,407 - BWI76_RS02995 0.45 -6.4 614,469 + BWI76_RS02995 0.52 -5.4 614,469 + BWI76_RS02995 0.52 -1.2 614,477 - BWI76_RS02995 0.52 -6.0 614,488 - BWI76_RS02995 0.54 -5.7 614,503 - BWI76_RS02995 0.55 -0.5 614,557 - BWI76_RS02995 0.61 -2.7 614,919 - -4.5 615,106 - -2.7 615,140 - -0.2 615,140 - -0.5 615,279 + -0.0 615,300 + -0.2 615,323 + BWI76_RS03005 0.15 -0.4 615,331 - BWI76_RS03005 0.17 +0.4 615,331 - BWI76_RS03005 0.17 -0.3 615,356 + BWI76_RS03005 0.22 -0.4 615,617 + BWI76_RS03005 0.80 -0.9 615,682 - -0.8 615,739 - +0.0 615,800 - -0.3 615,823 + -0.1 615,829 - -0.5 615,858 + -0.4 616,124 - BWI76_RS03010 0.22 -2.9 616,214 - BWI76_RS03010 0.31 -5.5 616,216 + BWI76_RS03010 0.32 -3.1 616,224 - BWI76_RS03010 0.32 -3.2 616,361 - BWI76_RS03010 0.46 -2.7 616,361 - BWI76_RS03010 0.46 -1.1 616,388 + BWI76_RS03010 0.49 -6.4 616,423 + BWI76_RS03010 0.52 -5.9 616,431 - BWI76_RS03010 0.53 -3.9 616,480 - BWI76_RS03010 0.58 -5.2 616,480 - BWI76_RS03010 0.58 -5.5 616,489 + BWI76_RS03010 0.59 -5.4 616,507 - BWI76_RS03010 0.61 -4.1 616,530 + BWI76_RS03010 0.63 -6.1 616,695 + BWI76_RS03010 0.79 -4.1
Or see this region's nucleotide sequence