Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02980 and BWI76_RS02985 are separated by 67 nucleotides BWI76_RS02985 and BWI76_RS02990 are separated by 112 nucleotides BWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotides
BWI76_RS02980: BWI76_RS02980 - type I magnesium transporting ATPase, at 610,237 to 612,945
_RS02980
BWI76_RS02985: BWI76_RS02985 - reactive intermediate/imine deaminase, at 613,013 to 613,399
_RS02985
BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973
_RS02990
BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921
_RS02995
Position (kb) 613
614 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 612.313 kb on + strand, within BWI76_RS02980 at 612.451 kb on - strand, within BWI76_RS02980 at 612.502 kb on + strand, within BWI76_RS02980 at 613.314 kb on - strand, within BWI76_RS02985 at 613.348 kb on + strand, within BWI76_RS02985 at 613.349 kb on - strand, within BWI76_RS02985 at 613.552 kb on - strand at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.917 kb on + strand, within BWI76_RS02990 at 613.941 kb on - strand at 614.067 kb on + strand at 614.248 kb on - strand, within BWI76_RS02995 at 614.303 kb on - strand, within BWI76_RS02995 at 614.318 kb on + strand, within BWI76_RS02995
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 612,313 + BWI76_RS02980 0.77 +0.1 612,451 - BWI76_RS02980 0.82 -0.1 612,502 + BWI76_RS02980 0.84 +0.4 613,314 - BWI76_RS02985 0.78 -0.8 613,348 + BWI76_RS02985 0.87 -1.7 613,349 - BWI76_RS02985 0.87 -1.3 613,552 - -0.7 613,889 + BWI76_RS02990 0.82 +0.1 613,889 + BWI76_RS02990 0.82 -0.6 613,889 + BWI76_RS02990 0.82 -0.5 613,917 + BWI76_RS02990 0.88 -0.9 613,941 - -1.3 614,067 + -4.6 614,248 - BWI76_RS02995 0.28 -5.8 614,303 - BWI76_RS02995 0.34 -3.4 614,318 + BWI76_RS02995 0.35 -5.1
Or see this region's nucleotide sequence