Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02985

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02980 and BWI76_RS02985 are separated by 67 nucleotidesBWI76_RS02985 and BWI76_RS02990 are separated by 112 nucleotidesBWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotides BWI76_RS02980: BWI76_RS02980 - type I magnesium transporting ATPase, at 610,237 to 612,945 _RS02980 BWI76_RS02985: BWI76_RS02985 - reactive intermediate/imine deaminase, at 613,013 to 613,399 _RS02985 BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973 _RS02990 BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921 _RS02995 Position (kb) 613 614Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 612.313 kb on + strand, within BWI76_RS02980at 612.451 kb on - strand, within BWI76_RS02980at 612.502 kb on + strand, within BWI76_RS02980at 613.314 kb on - strand, within BWI76_RS02985at 613.348 kb on + strand, within BWI76_RS02985at 613.349 kb on - strand, within BWI76_RS02985at 613.552 kb on - strandat 613.889 kb on + strand, within BWI76_RS02990at 613.889 kb on + strand, within BWI76_RS02990at 613.889 kb on + strand, within BWI76_RS02990at 613.917 kb on + strand, within BWI76_RS02990at 613.941 kb on - strandat 614.067 kb on + strandat 614.248 kb on - strand, within BWI76_RS02995at 614.303 kb on - strand, within BWI76_RS02995at 614.318 kb on + strand, within BWI76_RS02995

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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612,313 + BWI76_RS02980 0.77 +0.1
612,451 - BWI76_RS02980 0.82 -0.1
612,502 + BWI76_RS02980 0.84 +0.4
613,314 - BWI76_RS02985 0.78 -0.8
613,348 + BWI76_RS02985 0.87 -1.7
613,349 - BWI76_RS02985 0.87 -1.3
613,552 - -0.7
613,889 + BWI76_RS02990 0.82 +0.1
613,889 + BWI76_RS02990 0.82 -0.6
613,889 + BWI76_RS02990 0.82 -0.5
613,917 + BWI76_RS02990 0.88 -0.9
613,941 - -1.3
614,067 + -4.6
614,248 - BWI76_RS02995 0.28 -5.8
614,303 - BWI76_RS02995 0.34 -3.4
614,318 + BWI76_RS02995 0.35 -5.1

Or see this region's nucleotide sequence