Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02875

Experiment: RCH2_defined_glucose with MS_media 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02860 and BWI76_RS02865 are separated by 11 nucleotidesBWI76_RS02865 and BWI76_RS02870 are separated by 2 nucleotidesBWI76_RS02870 and BWI76_RS02875 are separated by 21 nucleotidesBWI76_RS02875 and BWI76_RS02880 are separated by 12 nucleotidesBWI76_RS02880 and BWI76_RS02885 are separated by 42 nucleotides BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360 _RS02860 BWI76_RS02865: BWI76_RS02865 - hypothetical protein, at 586,372 to 586,734 _RS02865 BWI76_RS02870: BWI76_RS02870 - hypothetical protein, at 586,737 to 587,036 _RS02870 BWI76_RS02875: BWI76_RS02875 - membrane protein, at 587,058 to 587,834 _RS02875 BWI76_RS02880: BWI76_RS02880 - membrane protein, at 587,847 to 588,491 _RS02880 BWI76_RS02885: BWI76_RS02885 - dihydroorotase, at 588,534 to 589,667 _RS02885 Position (kb) 587 588Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 586.112 kb on + strand, within BWI76_RS02860at 586.296 kb on - strand, within BWI76_RS02860at 586.357 kb on + strandat 586.410 kb on - strand, within BWI76_RS02865at 586.553 kb on + strand, within BWI76_RS02865at 586.553 kb on + strand, within BWI76_RS02865at 586.560 kb on + strand, within BWI76_RS02865at 586.566 kb on - strand, within BWI76_RS02865at 586.639 kb on + strand, within BWI76_RS02865at 586.654 kb on + strand, within BWI76_RS02865at 586.704 kb on - strandat 586.819 kb on - strand, within BWI76_RS02870at 586.881 kb on - strand, within BWI76_RS02870at 586.963 kb on + strandat 587.404 kb on + strand, within BWI76_RS02875at 587.446 kb on + strand, within BWI76_RS02875at 587.615 kb on + strand, within BWI76_RS02875at 587.640 kb on - strand, within BWI76_RS02875at 587.645 kb on - strand, within BWI76_RS02875at 587.670 kb on - strand, within BWI76_RS02875at 587.810 kb on - strandat 587.863 kb on - strandat 587.886 kb on + strandat 587.914 kb on - strand, within BWI76_RS02880at 587.942 kb on - strand, within BWI76_RS02880at 588.116 kb on - strand, within BWI76_RS02880at 588.118 kb on + strand, within BWI76_RS02880at 588.270 kb on + strand, within BWI76_RS02880at 588.369 kb on - strand, within BWI76_RS02880at 588.554 kb on - strandat 588.571 kb on + strandat 588.624 kb on - strandat 588.684 kb on + strand, within BWI76_RS02885at 588.773 kb on - strand, within BWI76_RS02885

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x
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586,112 + BWI76_RS02860 0.27 -0.5
586,296 - BWI76_RS02860 0.81 -0.1
586,357 + +0.3
586,410 - BWI76_RS02865 0.10 -1.6
586,553 + BWI76_RS02865 0.50 +0.1
586,553 + BWI76_RS02865 0.50 -0.3
586,560 + BWI76_RS02865 0.52 +0.4
586,566 - BWI76_RS02865 0.53 +0.4
586,639 + BWI76_RS02865 0.74 -0.1
586,654 + BWI76_RS02865 0.78 -3.6
586,704 - +0.5
586,819 - BWI76_RS02870 0.27 -0.2
586,881 - BWI76_RS02870 0.48 +0.1
586,963 + +0.3
587,404 + BWI76_RS02875 0.45 +0.9
587,446 + BWI76_RS02875 0.50 +0.3
587,615 + BWI76_RS02875 0.72 +0.1
587,640 - BWI76_RS02875 0.75 +0.2
587,645 - BWI76_RS02875 0.76 +0.1
587,670 - BWI76_RS02875 0.79 -0.2
587,810 - -0.1
587,863 - -0.0
587,886 + -1.3
587,914 - BWI76_RS02880 0.10 +2.0
587,942 - BWI76_RS02880 0.15 +0.1
588,116 - BWI76_RS02880 0.42 +0.3
588,118 + BWI76_RS02880 0.42 -0.5
588,270 + BWI76_RS02880 0.66 -0.3
588,369 - BWI76_RS02880 0.81 +0.3
588,554 - +0.4
588,571 + -0.1
588,624 - -0.5
588,684 + BWI76_RS02885 0.13 +0.1
588,773 - BWI76_RS02885 0.21 +0.6

Or see this region's nucleotide sequence