Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02170 and BWI76_RS02175 are separated by 364 nucleotides BWI76_RS02175 and BWI76_RS02180 are separated by 69 nucleotides BWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotides BWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotides
BWI76_RS02170: BWI76_RS02170 - MurR/RpiR family transcriptional regulator, at 451,272 to 452,159
_RS02170
BWI76_RS02175: BWI76_RS02175 - ribose 5-phosphate isomerase B, at 452,524 to 452,973
_RS02175
BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375
_RS02180
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745
_RS02190
Position (kb)
453
454 Strain fitness (log2 ratio)
-1
0
1 at 452.105 kb on + strand at 452.125 kb on - strand at 452.127 kb on + strand at 452.162 kb on - strand at 452.413 kb on + strand at 452.566 kb on - strand at 452.611 kb on - strand at 452.664 kb on - strand, within BWI76_RS02175 at 452.786 kb on - strand, within BWI76_RS02175 at 452.813 kb on - strand, within BWI76_RS02175 at 452.841 kb on - strand, within BWI76_RS02175 at 452.869 kb on - strand, within BWI76_RS02175 at 453.005 kb on + strand at 453.018 kb on - strand at 453.023 kb on + strand at 453.051 kb on + strand at 453.224 kb on - strand, within BWI76_RS02180 at 453.304 kb on + strand, within BWI76_RS02180 at 453.504 kb on + strand at 453.537 kb on + strand, within BWI76_RS02185 at 453.705 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185 at 454.169 kb on - strand at 454.274 kb on + strand, within BWI76_RS02190 at 454.343 kb on + strand, within BWI76_RS02190
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 452,105 + +0.2 452,125 - -0.3 452,127 + -0.4 452,162 - -0.0 452,413 + -0.4 452,566 - +0.3 452,611 - -1.4 452,664 - BWI76_RS02175 0.31 -0.3 452,786 - BWI76_RS02175 0.58 +0.2 452,813 - BWI76_RS02175 0.64 -0.2 452,841 - BWI76_RS02175 0.70 -0.3 452,869 - BWI76_RS02175 0.77 +0.1 453,005 + -1.0 453,018 - +0.0 453,023 + +0.3 453,051 + -0.2 453,224 - BWI76_RS02180 0.54 +0.5 453,304 + BWI76_RS02180 0.78 +0.1 453,504 + -1.0 453,537 + BWI76_RS02185 0.17 -0.3 453,705 - BWI76_RS02185 0.39 -0.2 453,803 - BWI76_RS02185 0.52 -0.2 453,803 - BWI76_RS02185 0.52 -0.6 453,830 + BWI76_RS02185 0.55 -0.5 453,848 - BWI76_RS02185 0.58 -0.5 453,943 + BWI76_RS02185 0.70 +0.1 454,169 - +0.4 454,274 + BWI76_RS02190 0.16 +0.1 454,343 + BWI76_RS02190 0.29 -0.5
Or see this region's nucleotide sequence