Experiment: RCH2_defined_glucose with MS_media 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01790 and BWI76_RS01795 are separated by 121 nucleotides BWI76_RS01795 and BWI76_RS01800 are separated by 282 nucleotides BWI76_RS01800 and BWI76_RS01805 are separated by 45 nucleotides BWI76_RS01805 and BWI76_RS01810 are separated by 8 nucleotides
BWI76_RS01790: BWI76_RS01790 - lysine-sensitive aspartokinase 3, at 365,131 to 366,480
_RS01790
BWI76_RS01795: BWI76_RS01795 - hypothetical protein, at 366,602 to 366,964
_RS01795
BWI76_RS01800: BWI76_RS01800 - glucose-6-phosphate isomerase, at 367,247 to 368,896
_RS01800
BWI76_RS01805: BWI76_RS01805 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 368,942 to 369,397
_RS01805
BWI76_RS01810: BWI76_RS01810 - N-acetyltransferase, at 369,406 to 369,915
_RS01810
Position (kb)
367
368
369 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 366.253 kb on + strand, within BWI76_RS01790 at 366.418 kb on + strand at 366.663 kb on + strand, within BWI76_RS01795 at 366.681 kb on + strand, within BWI76_RS01795 at 366.801 kb on + strand, within BWI76_RS01795 at 366.931 kb on + strand at 366.938 kb on - strand at 366.990 kb on - strand at 367.238 kb on + strand at 367.265 kb on + strand at 367.443 kb on - strand, within BWI76_RS01800 at 367.532 kb on + strand, within BWI76_RS01800 at 367.566 kb on - strand, within BWI76_RS01800 at 367.772 kb on - strand, within BWI76_RS01800 at 368.147 kb on + strand, within BWI76_RS01800 at 368.527 kb on + strand, within BWI76_RS01800 at 368.604 kb on + strand, within BWI76_RS01800 at 368.758 kb on - strand at 368.765 kb on - strand at 368.862 kb on + strand at 368.899 kb on + strand at 369.126 kb on + strand, within BWI76_RS01805 at 369.134 kb on - strand, within BWI76_RS01805 at 369.314 kb on + strand, within BWI76_RS01805 at 369.322 kb on + strand, within BWI76_RS01805 at 369.388 kb on + strand at 369.388 kb on + strand at 369.388 kb on + strand at 369.503 kb on - strand, within BWI76_RS01810 at 369.569 kb on - strand, within BWI76_RS01810 at 369.599 kb on + strand, within BWI76_RS01810 at 369.637 kb on - strand, within BWI76_RS01810 at 369.689 kb on - strand, within BWI76_RS01810 at 369.809 kb on - strand, within BWI76_RS01810 at 369.841 kb on - strand, within BWI76_RS01810 at 369.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with MS_media 0.5x remove 366,253 + BWI76_RS01790 0.83 +0.3 366,418 + -0.2 366,663 + BWI76_RS01795 0.17 -0.2 366,681 + BWI76_RS01795 0.22 -0.0 366,801 + BWI76_RS01795 0.55 -0.4 366,931 + -0.0 366,938 - +0.1 366,990 - -0.5 367,238 + +0.2 367,265 + -4.6 367,443 - BWI76_RS01800 0.12 -1.1 367,532 + BWI76_RS01800 0.17 -2.6 367,566 - BWI76_RS01800 0.19 -2.0 367,772 - BWI76_RS01800 0.32 -4.3 368,147 + BWI76_RS01800 0.55 -2.9 368,527 + BWI76_RS01800 0.78 -4.8 368,604 + BWI76_RS01800 0.82 -3.8 368,758 - +0.2 368,765 - -2.2 368,862 + -2.0 368,899 + -0.1 369,126 + BWI76_RS01805 0.40 -0.1 369,134 - BWI76_RS01805 0.42 +0.4 369,314 + BWI76_RS01805 0.82 -0.4 369,322 + BWI76_RS01805 0.83 -0.2 369,388 + -0.8 369,388 + +0.1 369,388 + -0.4 369,503 - BWI76_RS01810 0.19 +0.1 369,569 - BWI76_RS01810 0.32 -0.1 369,599 + BWI76_RS01810 0.38 -0.4 369,637 - BWI76_RS01810 0.45 -0.2 369,689 - BWI76_RS01810 0.55 -0.2 369,809 - BWI76_RS01810 0.79 -0.3 369,841 - BWI76_RS01810 0.85 -0.1 369,884 - -0.5
Or see this region's nucleotide sequence